Natural fruit beverages fortified by biologically active substances of grape vines


  • Lukáš Snopek Tomas Bata University in Zlí­n, Faculty of Technology, Department of Food Analysis and Chemistry, nám. T. G. Masaryka 5555, 760 01 Zlí­n, Czech Republic
  • Jiří­ Mlček Tomas Bata University in Zlí­n, Faculty of Technology, Department of Food Analysis and Chemistry, nám. T. G. Masaryka 5555, 760 01 Zlí­n, Czech Republic
  • Vlastimil Fic Tomas Bata University in Zlí­n, Faculty of Technology, Department of Food Analysis and Chemistry, nám. T. G. Masaryka 5555, 760 01 Zlí­n, Czech Republic
  • Irena Sytařová Tomas Bata University in Zlí­n, Faculty of Technology, Department of Food Analysis and Chemistry, nám. T. G. Masaryka 5555, 760 01 Zlí­n, Czech Republic
  • Soňa Škrovánková Tomas Bata University in Zlí­n, Faculty of Technology, Department of Food Analysis and Chemistry, nám. T. G. Masaryka 5555, 760 01 Zlí­n, Czech Republic



biologically active substances, natural stability, isolation of selected substances, natural beverages with or without alcohol


Based on the study of general knowledge of biochemical and all subsequent developmental studies of organic matter, especially products of grapevine and selected fruit products, a comprehensive study of processing technologies is prepared. Use of a combination of vine products and fruit products in the form of natural grapes. Beverages are researched and developed to be purely natural on the basis of grape musts, blue and white, either individually and again separately in targeted combinations, both biochemically, organoleptically and colorfully, with fruit sources. The core of grape value of biologically active substances is an integral and essential new part and condition of designing these beverages. Their increased biological values, which create the preconditions for containment and if properly managed on the basis of scientific knowledge, may in some cases almost result in the elimination of synthetic additives. It should be noted that
20 - 25% of the adult population suffers from many unexpected allergies, for example, to the sulphite content, although its content in the final product does not exceed the health-approved normatives. And there are many other, interrelated relationships. Beverages are technologically dealt with both without alcohol fermentation and with this fermentation, but only based on their compositional natural resources. They are therefore suitable for the entire population profile. The whole set contains 7 variants and a combination of natural beverages from different fruits. Including natural beverages with or without alcoholic fermentation from the must of white wine grapes, the juice of apple puree with those of biologically active substances from the products grapevine. Three months of monitoring and determination of basic (oenological) values and biologically active substances were performed on these products. The high-performance liquid chromatography method with a refractometric detector determined amount of sugar and alcohol, whilst titrating determined total and volatile acids and free sulfur dioxide. Yeast assimilable nitrogen, total anthocyanins and polyphenols were determined by spectrophotometry, antioxidant activity by DPPH and ABTS methods.


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AOAC. 1993. Methods of Analysis for Nutrition Labelling. Association of Official Analytical Chemists, Arlington, VA.

Baron, A., Dénes, J., Durier C. 2006. High-pressure treatment of cloudy apple juice. LWT - Food Science and Technology, vol. 39, no. 9, p. 1005-1013.

Duarte-Delgado, D., Narváez-Cuenca, C-E., Restrepo-Sánchez, L.-P., Kushalappa, A., Mosquera-Vásquez, T. 2015. Development and validation of a liquid chromatographic method to quantify sucrose, glucose, and fructose in tubers of Solanum tuberosum Group Phureja. J. Chromatogr., vol. 975, p. 18-23.

Giusti, M. R. Wrolstad. 2001. Characterization and Measurement of Anthocyanins by UV-Visible Spectroscopy. Current Protocols in Food Analytical Chemistry, vol. 00, no. 1, p. F1.2.1-F1.2.13.

Gump, B. H., Zoecklein, B. W., Fugelsang, K. C., Whinton, R. S. 2002. Comparison of analytical methods for prediction of prefermentation nutritional status of grape juice. American Journal of Enology and Viticulture, vol. 53, no. 4, p. 325-329.

Hernández, A., Pérez-Nevado, F., Ruiz-Moyano, S., Serradilla, M. J., Villalobos, M. C., Martín, A., Córdoba, M. G. 2018. Spoilage yeasts: What are the sources of contamination of foods and beverages? International journal of food microbiology, vol. 286, p. 98-110.

Hosu, A., Cristea, V-M., Cimpoiu, C. 2014. Analysis of total phenolic, flavonoids, anthocyanins and tannins content in Romanian red wines: Prediction of antioxidant activities and classification of wines using artificial neural networks. Food chemistry, vol. 150. p. 113-118.

Hurtado, I., Caldu, P., Gonzalo, A., Ramon, J.M., Minguez, S., Fiol, C. 1997. Antioxidative capacity of wine on human LDL oxidation in vitro: effect of skin contact in winemaking of white wine. J. Agric. Food Chem., vol. 45. p. 1283-1289.

Ivanova, V., Petruseva, D., Mitrev, S. 2015. Methods for determination of SO2 and reducing sugars in wines and alcoholic beverages. Yearbook of Faculty of Agriculture, vol. 13, no. 1, p. 119-127.

Lachman, J., Šulc, M., Faitová, K., Pivec, V. 2009. Major factors influencing antioxidant contents and antioxidant activity in grapes and wines. International Journal of Wine Research, vol. 1, no. 1, p. 101-121.

Mlček, J., Juríková, T., Škrovánková, S., Paličková, M., Orsavová, J., Mišurcová, L., Hlaváčová, I., Sochor, J., Sumczynski, D. 2016. Polyphenol content and antioxidant capacity of fruit and vegetable beverages processed by different technology methods. Potravinarstvo Slovak Journal of Food Sciences, vol. 10, no. 1, p. 512-517.

Nurgle, C., Pickering, G. 2005. Contribution of glycerol, ethanol and sugar to the perception of viscosity and density elicited by model white wines. Journal of texture studies, vol. 36, no. 3, p. 303-323.

OIV. 2O15. International Organisation of Vine and Wine, Compendium of international Methods of wine and must analysis [online] s.a. [cit. 2019 -01-19]. Available at:

Orsavová, J., Hlaváčová, I., Mlček, J., Snopek, L., Mišurcová, L. 2019. Contribution of phenolic compounds, ascorbic acid and vitamin E to antioxidant activity of currant (Ribes L.) and gooseberry (Ribes uva-crispa L.) fruits. Food Chemistry, vol. 284, p. 323-333.

Paixao, N., Perestrelo, R., Marques J. C., Camara, J. S. 2007. Relationship between antioxidant capacity and total phenolic content of red, rose and white wines. Food Chem., 105. p. 204-214.

Petrovica, G., Kiddb, M., Buicaa, A. 2018. A statistical exploration of data to identify the role of cultivar and origin in the concentration and composition of yeast assimilable nitrogen. Food Chemistry, vol. 276, p. 528-537.

Pickering, G., Heatherbell, D., Barnes, D. Vanhanen, L. P. 1998. The effect of ethanol concentration on the temporal perception ofviscosity and density in white wine. Am. J. Enol. Viticult., vol. 49, p. 306-318.

Qin, Z., Petersen, M., Bredie, W., L. P. 2018. Flavor profiling of apple ciders from the UK and Scandinavian region. Food Research International, vol. 105, p. 713-723.

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, vol. 26, p. 1231-1237.

Ricci, A., Parpinello, G. P., Versari, A. 2017. Modelling the evolution of oxidative browning during storage of white wines: effects of packaging and closures. International journal of food science & technology, vol. 52, no. 2, p. 472-479.

Rop, O., Mlček, J., Juríková, T., Valšíková, M., Sochor, J., Řezníček, V., Kramářová, D. 2010. Phenolic content, antioxidant capacity, radical oxygen species scavenging and lipid peroxidation inhibiting activities of extracts of five black chokeberry (Aronia melanocarpa (Michx.) Elliot) cultivars. Journals of Medicinal Plants Research, vol. 4, p. 2431-2437.

Sims, A., 1995. HPLC analysis of sugars in foods containing salt. J. Agric. Food Chem., vol. 43, no. 2, p. 377-380.

Singleton, V. L., Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, vol. 16, no. 3, p. 144-158.

Snopek, L., Mlček, J., Fic, V., Hlaváčová, I., Škrovánková, S., Fišera, M., Velichová, H., Ondrášová, M. 2018. Interaction of polyphenols and wine antioxidants with its sulfur dioxide preservative. Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 180-185.

Steidl, R. 2002. Cellar holding (Sklepní hospodářství). Valtice, Czech Republic : Radix, 308 p. ISBN 80-903201-0-4. (In Czech)

Tarko, T., Duda-Chodak, A., Semik, D., Nycz, M. 2015. The use of fruit extracts for production of beverages with high antioxidative activity. Potravinarstvo, vol. 9, no. 1, p. 280-283.

Valdramidis, V. P., Graham, W. D., Beatie, A., Linton, M. Mckay, A. Fearon, A. M., Patterson, M. F. 2009. Defining the stability interfaces of apple juice: implications on the optimisation and design of high hydrostatic pressure treatment. Innovative Food Science & Emerging Technologies, vol. 10, no. 4, p. 396-404.

Vrancheva, R., Ivanov, I., Aneva, I., Stoyanova, M., Pavlov, A. 2018. Food additives and bioactive substances from in vitro systems of edible plants from the Balkan peninsula. Engineering in Life Sciences, vol. 18, no. 11, p. 799-806.

Wells, A., Osborne, J. P. 2011. Production of SO2 binding compounds and SO2 by Saccharomyces during alcoholic fermentation and the impact on malolactic fermentation. South African Journal of Enology and Viticulture, vol. 32, no. 2, p. 267-279.

Wolfe, K., Wu, X. Liu, R. H. 2003. Antioxidant activity of apple peels. Journal of Agricultural and Food Chemistry, vol. 51, no. 3, p. 609-614.

Yu, T., Zhou, Y. J., Huang, M., Liu, Q., Pereira, R., David, F., Nielsen, J. 2018. Reprogramming yeast metabolism from alcoholic fermentation to lipogenesis. Cell, vol. 174, no. 6, p. 1549-1558.



How to Cite

Snopek, L. ., Mlček, J. ., Fic, V. ., Sytařová, I. ., & Soňa Škrovánková. (2019). Natural fruit beverages fortified by biologically active substances of grape vines. Potravinarstvo Slovak Journal of Food Sciences, 13(1), 167–173.

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