Expression pattern of thaumatin in the selected red varieties of Vitis vinifera, L.

Authors

  • Jana Žiarovská Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Department of Genetics and Plant Breeding, Tr. A. Hlinku 2, 949 76 Nitra https://orcid.org/0000-0002-0005-9729
  • Veronika Fialková Slovak University of Agriculture, Research centre AgroBioTech, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia https://orcid.org/0000-0002-3361-0724
  • Lucia Zamiešková Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Plant Breeding, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia https://orcid.org/0000-0001-7434-2677
  • Jana Bilčí­ková Slovak University of Agriculture, Research centre AgroBioTech, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia https://orcid.org/0000-0003-1738-1113
  • Lucia Zeleňáková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia https://orcid.org/0000-0003-1387-7410
  • Miroslava Kačániová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia https://orcid.org/0000-0002-4460-0222

DOI:

https://doi.org/10.5219/1057

Keywords:

thaumatin, expression, red varieties, Vitis vinifera L.

Abstract

Vitis vinifera L. is a specie that is adapted to a very variable range of climates, from cold up to the desert one, but especially it grows in the temperate Mediterranean regions and continental areas of Europe. Grape is a widespread consumed fruit as well as processed to musts, juices or wine. The health beneficial effects of grapes and wine are very well known due to their high nutritional value and unique phytochemical composition. Despite many health protective and beneficial effects of Vitis vinifera, a part of population suffer to allergic reactions to this fruit. Allergens of wine and grapes are: endochitinases, lipid-transfer protein and thaumatin. Thaumatin is a protein having a sweet taste belonging to the PR5-like proteins.  These proteins are very difersified in their functions and were described to be involved in stress responses and fruit ripening, but are expressed in healthy grape fruits in a constitutive manner and needn´t to be expressed only as a answer to the stress. Thaumatin is a minor allergen in grape, but belonging to the suspected panallergens relevant to the food cross-allergy induction, its importance is quite high. Another importance of this protein is a technological one, as reported to aggregate in wine to form a visible haze unless removed prior to bottling. In this study, expression of thaumatin-like allergen was analysed in the grapes of selected varieties. Grapes of four red varieties of Vitis vinifera, L. were obtained in the season 2017 in the Sabo winery  that belongs to the Malokarpatská wine region. Fresh maturated grapes of varieties Alibernet, Cabernet Sauvignon , Frankovka modrá and Dornfelder were analysed. Expression changes of thaumatin was calculated by delta delta Ct method.  Dornfelder was found as to have the lowest activity in thaumatin-like gene activity,  mainly when comparing to the Cabernet Sauvignon and Frankovka modrá. Alibernet, on the other side, has the expression level of thaumatin very similar when comparing to the Cabernet Sauvignon and Frankovka modrá.

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Published

2019-06-28

How to Cite

Žiarovská, J. ., Fialková, V. ., Zamiešková, L. ., Bilčí­ková, J. ., Zeleňáková, L., & Kačániová, M. (2019). Expression pattern of thaumatin in the selected red varieties of Vitis vinifera, L. Potravinarstvo Slovak Journal of Food Sciences, 13(1), 547–552. https://doi.org/10.5219/1057

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