Evaluation of genetic diversity of edible honeysuckle monitored by RAPD in relation to bioactive substances

Authors

  • Marcela Cehula Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Department of Botany and Genetics, Nábrežie mládeže 91, 949 74 Nitra, Slovak Republic, Tel.: +421376408581 https://orcid.org/0000-0002-8048-1144
  • Tünde Jurí­ková Constantine the Philosopher University, Faculty of Central European Studies, Institute for Teacher Training, Dražovská 4, 949 74 Nitra, Slovakia, Tel.: +421376408855 https://orcid.org/0000-0002-8286-8262
  • Jana Žiarovská Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Plant Breeding, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414244 https://orcid.org/0000-0002-0005-9729
  • Jiří­ Mlček Tomas Bata University in Zlí­n, Faculty of Technology, Department of Food Analysis and Chemistry, nám. T.G. Masaryka 5555, 760 01 Zlí­n, Czech Republic, Tel.: +420576033030 https://orcid.org/0000-0002-5753-8560
  • Matúš Kyseľ Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Genetics and Plant Breeding, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421907045799 https://orcid.org/0000-0002-1679-391X

DOI:

https://doi.org/10.5219/1139

Keywords:

honeysuckle, RAPD, DPPH, Folin-Ciocalteu, total polyphenols content

Abstract

The aim of this study was clarifying the relation between genetic diversity of edible honeysuckle (Lonicera kamtschatica) and the major group of biologically active substances as total polyphenols content (TPC) including antioxidant activity (AO). Fruits of edible honeysuckle becomes more and more popular, especially in Europe. The current status of research on polyphenolic compounds in the berries of edible honeysuckle and their biological effects, including recommended utilization, are reviewed.The biological material including 14 cultivars of the edible honeysuckle (´Zoluška´, ´Amfora´, ´Pruhonický 44´, ´Vasilijevsky´, ´Moskovskaja´, ´Vojtek´, ´Sinoglaska´, ´Altaj´, ´Lipnická´, ´Kamčadalka´, ´Sinaja Ptica´, ´Fialka´, ´Modrý Triumf´, and ´Leningradský velikán´) originated from Czech republic (Žabcice near Brno). The content of TPC and AO were determined by location and its soil-climatic conditions and these environmental circumstances determines the RAPD profiles of analysed honeysuckle acessions, too. DPPH method was used to analyze AO and Folin-Ciocalteu method was used to determine TPC. The results of experiment showed that the highest value of AO was determined at the cultivars ´Zoluška´ (81.04 mg.L-1) and the lowest was measured in ´Kamčadalka´ (54.122 mg.L-1).  On the contrary, the highest content of TPC was determined at the cultivar ´Kamčadalka´ (51.09 mg.L-1) and the lowest value was measured at the cultivar ´Pruhonický 44´ (21.65 mg.L-1). Phylogenetic trees were similar in genetic distance. The  content of TPC and AO were  not statistically significant in relation to cultivar. The analyzed cultivars of the edible honeysuckle were separated in 4 clusters according to used primers. In both  gel images, the amplicon size ranged from 100 to 1,500 bp. We found that genetic diversity was partially related to content of total polyphenolic substances and antioxidant activity. Based on phylogenetic trees we have stated that variety ´Lipnická´, ´Sinoglaska´, ´Altaj´, ´Leningradský velikán´, ´Modrý Triumf´, ´Sinaja Ptica´ and ´Kamčadalka´ were grouped in the similar cluster. The highest genetic distance was determined at the variety ´Vasilijevskaja´ and ´Amfora´. In the same way, there were variety ´Vojtek´, ´Fialka´ and ´Zoluška´.

Downloads

Download data is not yet available.

References

Becker, R., Dashbaldan, S., Pączkowski, C., Golis, T., Szakiel, A. 2019. Comparison of steroids and triterpenoids in leaf cuticular waxes of selected Polish and Russian cultivars and genotypes of edible honeysuckle. Phytochemistry Letters, vol. 30, p. 238-244. https://doi.org/10.1016/j.phytol.2019.01.009

Ding, G., Wang, Y., Liu, A., Hou, Y., Zhang, T., Bai, G., Liu, Ch. 2017. From chemical markers to quality markers: an integrated approach of UPLC/Q-TOF, NIRS, and chemometrics for the quality assessment of honeysuckle buds. RSC Advances, vol. 7, no. 36, p. 22034-22044. https://doi.org/10.1039/C6RA28152D

Fu, J., Yang, L., Khan, A., Mei, Z. 2013. Genetic characterization and authentication of Lonicera japonica Thunb. by using improved RAPD analysis. Molecular Biology Reports, vol. 40, no. 10, p. 5993-5999. https://doi.org/10.1007/s11033-013-2703-3

Gazdík, Z., Reznicek, V., Adam, V., Zitka, O., Jurikova, T., Krska, B., Matuskovic, J., Plsek, J., Saloun, J., Horna, A., Kizek, R. 2008. Use of liquid chromatography with electrochemical detection for the determination of antioxidants in less common fruits. Molecules, vol. 13, no. 11, p. 2823-2836. https://doi.org/10.3390/molecules131102823

Handa, T., Kita, K., Wongsawad, P., Kurashige, Y., Yukawa, T. 2006. Molecular phylogeny assisted breeding of ornamentals. Journal of Crop Improvement, vol. 17, no. 1-2, p. 51-68. https://doi.org/10.1300/J411v17n01_03

Heinrich, J., Švarcová, I., Valentová, K. 2008. Plody Lonicera Caerulea: Perspektivní funkční potravina a zdroj biologicky aktivních látek (Fruits of Lonicera Caerulea: A perspective functional food and source of biologically active substances). Chemické Listy, vol. 102, p. 245-254.

Holubec, V., Smekalova, T., Leisova-Svobodova, L. 2019. Morphological and molecular evaluation of the Far East fruit genetic resources of Lonicera caerulea L.-vegetation, ethnobotany, use and conservation. Genetic Resources and Crop Evolution, vol. 66, no. 1, p. 121-141. https://doi.org/10.1007/s10722-018-0701-y

Cheng, J. L., Li, J., Qiu, Y. M., Wei, C. L., Yang, L. Q., Fu, J. J. 2016. Development of novel SCAR markers for genetic characterization of Lonicera japonica from high GC-RAMP-PCR and DNA cloning. Genetics and Molecular Research, vol. 15, no. 2, p. 1-12. https://doi.org/10.4238/gmr.15027737

Jiang, C., Yuan, Y., Chen, M., Huang, L. 2013. Molecular authentication of multi-species honeysuckle tablets. Genetics and Molecular Research, vol. 12, no. 4, p. 4827-4835. https://https://doi.org/10.4238/2013.October.22.2

Juríková, T., Rop, O., Mlcek, J., Sochor, J., Balla, S., Szekeres, L., Hegedusova, A., Hubalek, J., Adam, V., Kizek, R. 2012. Phenolic Profile of Edible Honeysuckle Berries (Genus Lonicera) and Their Biological Effects. Molecules, vol. 17, no. 1, p. 61-79. https://doi.org/10.3390/molecules17010061

Juríková, T., Sochor, J., Mlcek, J., Balla, S., Ercisli, S., Durisova, L., Kynicky, J. 2014. Polyphenolic Compounds and Antioxidant Activity in Berries of Four Russian Cultivars of Lonicera kamtschatica (Sevast.) Pojark. Erwerbs-Obstbau, vol. 56, no. 4, p. 117-122. https://doi.org/10.1007/s10341-014-0215-5

Kaczmarska, E., Gawroński, J., Dyduch-Siemińska, M., Najda, A., Marecki, W., Żebrowska, J. 2015. Genetic diversity and chemical characterization of selected Polish and Russian cultivars and clones of blue honeysuckle (Lonicera caerulea). Turkish Journal of Agriculture and Forestry, vol. 39, p. 394-402. https://doi.org/10.3906/tar-1404-149

Kucharska, A. Z., Sokół-Łętowska, A., Oszmiański, J., Piórecki, N., Fecka, I. 2017. Iridoids, Phenolic Compounds and Antioxidant Activity of Edible Honeysuckle Berries (Lonicera caerulea var. kamtschatica Sevast.). Molecules, vol. 22, no. 3, p. 1-20. https://doi.org/10.3390/molecules22030405

Lima, A. T. B., de Souza, V. A. B., Gomes, R. L. F., Lima, P. S. C. 2011. Molecular characterization of caja´, Spondias mombin (Anacardiaceae), by RAPD markers. Genetics and Molecular Research, vol. 10, no. 4, p. 2893-2904. https://doi.org/10.4238/2011.November.25.1

Matuškovič, J., Benediková, D., Krška, B., Sasková, H., Nečas, T., Gorina, V. M., Žebentjajeva, T. M., Glasa, M., Paulen, O., Pintér, E., Marenčík, A., Juríková, T., Hríbik, J., Owais, S. J. E., Dunca, J., Hlaváčová, Z., Hudec, J., Kobida, Ľ., Bystrická, J., Trebichalský, P., Musilová, J., Kobidová, R., Horčin, V., Jóžeffiová, E., Orinčák, J., Jurčák, S., Řezníček, V., Marková, R., Salaš, P., Svitáčková, B. 2003. Agrobiologické faktory ovplyvňujúce úspešnosť pestovania marhúľ a zemolezu kamčatského (Agrobiological factors affecting the success of the cultivation of apricots and honeysuckle kamtschatica). 1st ed. Nitra, Slovakia : Slovak University of Agriculture, 219 p. ISBN-80-8069-289-0. (In Slovak)

Matuškovič, J., Juríková, T., Jurík, I., Šimko, J., Gazdík, Z. 2009. The content of anthocyanins and ascorbic acid in the genofond of 22 clones of Lonicera kamtschatica (Sevast.) Pojark. GERDA/25. Agriculture (Poľnohospodárstvo), vol. 55, no. 2, p. 88-94.

Naugžemys, D., Žilinskaité, S., Skridaila, A., Žvingila, D. 2014. Phylogenetic analysis of the polymorphic 4x species complex Lonicera caerulea (Caprifoliaceae) using RAPD markers and noncoding chloroplast DNA sequences. Biologia, vol. 69, no. 5, p. 585-593. https://doi.org/10.2478/s11756-014-0345-0

Nei, M., Li, W. H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA, vol. 76, p. 5269-5273.

Paulová, H., Bochořáková, H., Táborská, E. 2004. Metody stanovení antioxidační aktivity přírodních látek in vitro (Methods of determination of antioxidant activity of natural substances in vitro). Chemické Listy, vol. 98, p. 174-179.

Quiros, A. R. B., Frecha-Ferreiro, S., Vidal-Pérez, A. M., López-Hernández, J. 2010. Antioxidant compounds in edible brown seaweeds. European Food Research and Technology, vol. 231, no. 3, p. 495-498. https://doi.org/10.1007/s00217-010-1295-6

Rogers, S. O., Bendich, A. J. 1994. Extraction of total cellular DNA from plants, algae and fungi. Plant Mol. Biol. Manual, p. 183-190. https://doi.org/10.1007/978-94-011-0511-8_12

Rop, O., Jurikova, T., Sochor, J., Mlcek, J., Kramarova, D. 2011a. Antioxidant capacity, scavenging radical activity and selected chemical composition of native apple cultivars from Central Europe. Journal of Food Quality, vol. 34, no. 3, p. 187-194. https://doi.org/10.1111/j.1745-4557.2011.00387.x

Rop, O., Řezníček, V., Mlcek, J., Juríková, T., Balík, J., Sochor, J., Kramarová, D. 2011b. Antioxidant and radical oxygen species scavenging activities of 12 cultivars of blue honeysuckle fruit. Horticultural Science, vol. 38, no. 2, p. 63-70. https://doi.org/10.17221/99/2010-HORTSCI

Senica, M., Bavec, M., Stampar, F., Petkovsek, M. M. 2018. Blue honeysuckle (Lonicera caerulea subsp. edulis (Turcz. ex Herder) Hultén.) berries and changes in their ingredients across different locations. Journal of the Science of Food and Agriculture, vol. 98, no. 9, p. 3333-3342. https://doi.org/10.1002/jsfa.8837

Sochor, J., Jurikova, T., Pohanka, M., Skutkova, H., Baron, M., Tomaskova, L., Balla, S., Klejdus, B., Pokluda, R., Mlcek, J., Trojakova, Z., Saloun, J. 2014. Evaluation of Antioxidant Activity, Polyphenolic Compounds, Amino Acids and Mineral Elements of Representative Genotypes of Lonicera edulis. Molecules, vol. 19, no. 5, p. 6504-6523. https://doi.org/10.3390/molecules19056504

Sun, Z., Gao, T., Yao, H., Shi, L., Zhu, Y., Chen, S. 2011. Identification of Lonicera japonica and its related species using the DNA barcoding method. Planta medica, vol. 77, no. 3, p. 301-306. https://doi.org/10.1055/s-0030-1250324

Vivodík, M., Saadaoui, E., Balážová, Ž., Gálová, Z., Petrovičová, L. 2019. Genetic diversity in Tunisian castor genotypes (Ricinus communis L.) detected using RAPD markers. Slovak Journal of Food Sciences, vol. 13, no. 1, p. 294-300. https://doi.org/10.5219/1116

Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., Tingey, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, vol. 18, no. 22, p. 6531-6535. https://doi.org/10.1093/nar/18.22.6531

Zhao, L., Li, S., Zhao, L., Zhu, Y., Hao, T. 2015. Antioxidant activities and major bioactive components of consecutive extracts from blue honeysuckle (Lonicera caerulea L.) cultivated in China. Journal of Food Biochemistry, vol. 39, no. 6, p. 653-662. https://doi.org/10.1111/jfbc.12173

Žlabur, Š. J., Colnar, D., Voća, S., Lorenzo, J. M., Munekata, P. E. S., Barba, F. J., Dobričavić, N., Galić, A., Dujmić, F., Pliestić, S., Brčić, M. 2019. Effect of ultrasound pre-treatment and drying method on specialized metabolites of honeyberry fruits (Lonicera caerulea var. kamtschatica). Ultrasonics Sonochemistry, vol. 56, p. 372-377. https://doi.org/10.1016/j.ultsonch.2019.04.034

Published

2019-06-28

How to Cite

Cehula, M., Jurí­ková, T., Žiarovská, J., Mlček, J., & Kyseľ, M. (2019). Evaluation of genetic diversity of edible honeysuckle monitored by RAPD in relation to bioactive substances. Potravinarstvo Slovak Journal of Food Sciences, 13(1), 490–496. https://doi.org/10.5219/1139

Most read articles by the same author(s)

1 2 3 4 5 > >>