Simultaneous determination of sweeteners and preservatives in beverages by HPLC-DAD-ELSD

Authors

  • Pavel Diviš Brno University of Technology, Faculty of Chemistry, Department of Food Chemistry and Biotechnology, Purkynova 118, 612 00 Brno, Czech Republic, Tel. +420541149454 https://orcid.org/0000-0001-6809-0506
  • Zuzana Jurečková Brno University of Technology, Faculty of Chemistry, Department of Food Chemistry and Biotechnology, Purkynova 118, 612 00 Brno, Czech Republic, Tel. +420541149425
  • Milena Vespalcová Brno University of Technology, Faculty of Chemistry, Department of Food Chemistry and Biotechnology, Purkynova 118, 612 00 Brno, Czech Republic, Tel. +420541149425
  • Jaromí­r Poří­zka Brno University of Technology, Faculty of Chemistry, Department of Food Chemistry and Biotechnology, Purkynova 118, 612 00 Brno, Czech Republic, Tel. +420541149320
  • Lenka Punčochářová Brno University of Technology, Faculty of Chemistry, Department of Food Chemistry and Biotechnology, Purkynova 118, 612 00 Brno, Czech Republic, Tel. +420732703682 https://orcid.org/0000-0002-0468-6519

DOI:

https://doi.org/10.5219/1339

Keywords:

sweeteners, preservatives, beverage, chromatography, DAD ELSD detectors

Abstract

People suffering from diabetes or being overweight must severely reduce their sugar use, often seeking food with sweeteners. Often, sugar is replaced by non-nutritious sweeteners in beverages, which also contain several other substances like vitamins, caffeine, amino acids, phenolic compounds and thus increasing the shelf life of the beverages is additionally treated with the addition of preservatives. As the concentration of additives in food (including beverages) is determined by the legislation in force, it is necessary to have an appropriate analytical method for food control. Since artificial sweeteners and preservatives are very different substances, they are determined separately using different HPLC methods. In this work HPLC method combining the advantages of specific (diode array detector, DAD) and universal (evaporative light scattering detector, ELSD) detector was validated and used for simultaneous determination of benzoic acid, sorbic acid, aspartame, acesulfame K, saccharin, sucralose and steviol glycosides in sugar-free beverages. The proposed analytical method showed good linearity, precision, and accuracy. Measured limits of detection (0.6 – 11.8 mg.dm-3 depending on the analyte) were sufficient to analyze 5-times diluted beverage samples. The validated method has been successfully used for the simultaneous analysis of artificial sweeteners and preservatives in beverage samples (energy drinks, ice teas, carbonated drinks). Except for steviol glycosides, the concentration of monitored substances in beverages did not exceed the maximum permitted concentrations given in the valid legislation.

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Published

2020-10-28

How to Cite

Diviš, P., Jurečková, Z., Vespalcová, M. ., Poří­zka, J., & Punčochářová, L. (2020). Simultaneous determination of sweeteners and preservatives in beverages by HPLC-DAD-ELSD. Potravinarstvo Slovak Journal of Food Sciences, 14, 881–886. https://doi.org/10.5219/1339

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