Fruit red colorants impact on the antiradical activity determined by DPPH method

Authors

  • Ondrej Hegedűs J. Selye University, Faculty of Economics and Informatics, Department of Management, Hradná 21, 945 01 Komárno, Slovakia, Tel.: +421 35 32 60 865 https://orcid.org/0000-0002-0643-7014
  • Boglárka Borovicza J. Selye University, Faculty of Education, Department of Chemistry, Bratislavská cesta 3322, 945 01 Komárno, Slovakia, Tel.: +421 35 32 60 865 https://orcid.org/0000-0003-4266-7869
  • Katarí­na Szarka J. Selye University, Faculty of Education, Department of Chemistry, Bratislavská cesta 3322, 945 01 Komárno, Slovakia, Tel.: +421 35 32 60 683 https://orcid.org/0000-0002-0741-3364
  • Andrea Vargová J. Selye University, Faculty of Education, Department of Chemistry, Bratislavská cesta 3322, 945 01 Komárno, Slovakia, Tel.: +421 35 32 60 873
  • Dániel Reménység J. Selye University, Faculty of Education, Department of Chemistry, Bratislavská cesta 3322, 945 01 Komárno, Slovakia, Tel.: +421 35 32 60 683 https://orcid.org/0000-0002-2745-9351
  • Adriána Maťová Slovak University of Agriculture in Nitra, Faculty of Horticulture and Landscape Engineering, Department of Vegetable Production, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421 37 641 4262 https://orcid.org/0000-0003-3325-0834
  • Barnabás Czibor J. Selye University, Faculty of Education, Department of Chemistry, Bratislavská cesta 3322, 945 01 Komárno, Slovakia, Tel.: +421 35 32 60 873
  • Alžbeta Hegedüsová Slovak University of Agriculture in Nitra, Faculty of Horticulture and Landscape Engineering, Department of Vegetable Production, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421 37 641 4712

DOI:

https://doi.org/10.5219/1374

Keywords:

DPPH method, antiradical activity, dependence, ascorbic acid, polyphenols

Abstract

This work is focused on the determination of antiradical activity (ARA) by the method of free radical scavenging of 2,2-Diphenyl-1-picrylhydrazyl (DPPH). Since the DPPH solution is intense purple-coloured and absorbs at a wavelength of 517 nm, similar to the anthocyanin colorants of fruits, the modification of the method examined the effect of the colour of the sample extracts on the result of the ARA determination. Statistical evaluation of the results of the analyses using Youden's graphing method revealed that the two compared method adjustments gave consistent results over the observed range of antiradical activity. It also showed that there was no statistically significant difference between the mean ARA values obtained by the two treatments. Investigation of the effect of the evaluated fruit components revealed a strong correlation between the content of ascorbic acid and ARA in samples containing no anthocyanin colorants. For the fruit samples studied, ARA values showed a strong correlation with polyphenol content.

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References

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Published

2020-07-28

How to Cite

Hegedűs, O., Borovicza, B., Szarka, K., Vargová, A., Reménység, D., Maťová, A., Czibor, B., & Hegedüsová, A. (2020). Fruit red colorants impact on the antiradical activity determined by DPPH method. Potravinarstvo Slovak Journal of Food Sciences, 14, 473–482. https://doi.org/10.5219/1374

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