Eva Green Real-time PCR used to detect celery as an allergen in food

Authors

  • Ondrej Škultéty Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
  • Andrea Jurčáková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia

DOI:

https://doi.org/10.5219/138

Keywords:

apium graveolens, food analysis, detection limit, polymerase chain reaction

Abstract

EvaGreen®  Real-Time PCR method has been used for celery(Apium graveolens) allergen detection. A primer designed in mannitol dehydrogenase gene region has been used for specific celery identification in sample. The results show possibility to create calibration curve using artificially adulterated samples. The increasing variability between parallel calibration of celery samples has been observed from 0.1 % to 100%. Detection limit has been set to value 0.1% in celery representing 1000 ppm. Fluorescent signal has been presented even in samples with lower percentage addition of celery but these samples have been excluded according to unspecific

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Published

2011-03-13

How to Cite

Škultéty, O. ., & Jurčáková, A. . (2011). Eva Green Real-time PCR used to detect celery as an allergen in food. Potravinarstvo Slovak Journal of Food Sciences, 5(2), 70–72. https://doi.org/10.5219/138