Effect of modelled stress and adaptogens on microstructural characteristics of pork from fast-growing hybrid animals

Authors

  • Anastasia Semenova V. M. Gorbatov Federal Research Center for Food Systems of RAS, Talalikhina st. 26, 109316, Moscow, Russia, Tel.: +74956766161
  • Tatyana Kuznetsova V. M. Gorbatov Federal Research Center for Food Systems of RAS, Talalikhina st. 26, 109316, Moscow, Russia, Tel.: +74956766161
  • Victoria Nasonova V. M. Gorbatov Federal Research Center for Food Systems of RAS, Talalikhina st. 26, 109316, Moscow, Russia, Tel.: +74956766551 https://orcid.org/0000-0001-7625-3838
  • Roman Nekrasov L. K. Ernst Federal Science Center for Animal Husbandry Podolsk, Moscow Region, Russia, Tel.: +74967651277 https://orcid.org/0000-0003-4242-2239
  • Nadezhda Bogolubova L. K. Ernst Federal Science Center for Animal Husbandry Podolsk, Moscow Region, Russia, Tel.: +74967651169 https://orcid.org/0000-0002-0520-7022

DOI:

https://doi.org/10.5219/1388

Keywords:

pork, muscle tissue, myopathy, adaptogen, stress

Abstract

This research aimed to study the effect of modelled technological stress and the introduction of selenium and dihydroquercetin (DHQ) into pig diets on the microstructure of M. longissimus dorsi muscle tissue. The in vivo experiment was carried out on 36 hybrid young barrows (Large White x Landrace) x Duroc) with an initial live weight of 34 – 36 kg until they reached a weight of not less than 110 kg. The animals were divided into four groups: 1 (C-) – pigs did not receive adaptogens and were not subjected to modelled technological stress; 2 (C+) – pigs did not receive adaptogens but were subjected to stress via relocation of animals; 3 (С+Se) – pigs were subjected to stress and received 0.2 mg Se.kg-1 feed as selenium proteinate in addition to their diet; 4 (С+DHQ) – pigs were subjected to stress and received 32 mg DHQ.kg-1 feed in addition to their diet. The best results regarding the muscle tissue condition were recorded in the musle  L. dorsi samples were taken from the carcasses of group 4 (С+DHQ). Analysis of variance using the Fisher–Snedecor test confirmed that addition of adaptogens led to an improvement of the pH24 value (at p = 0.05, f observed  = 5.90 >fcritical = 4.17) and moisture-holding capacity (at p = 0.05, f observed  = 3.04 >fcritical = 2.92). The effect of long-term addition of DHQ to pig diets (78 days) on the condition of muscle tissue was studied for the first time, which allowed us to conclude its role in the prevention of myopathic changes in the muscle fibre structure.

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References

Calvo, L., Toldrá, F., Aristoy, M. C., López-Bote, C. J., Rey, A. I. 2016. Effect of dietary organic selenium on muscle proteolytic activity and water-holding capacity in pork. Meat Science, vol. 121, p. 1-11. https://doi.org/10.1016/j.meatsci.2016.05.006

Calvo, L., Toldrá, F., Rodríguez, A. I., López-Bote, C., Rey, A. I. 2017. Effect of dietary selenium source (organic vs. mineral) and muscle pH on meat quality characteristics of pigs. Food Science & Nutrition, vol. 5, no. 1, p. 94-102. https://doi.org/10.1002/fsn3.368

Cooper, B. J., Valentine, B. A. 2015. Muscle and Tendon. In: Jubb, Kennedy and Palmer. Pathology of Domestic Animals. E-Book: 3-Volume Set. 6th ed. Oxford, UK : Elsevier Health Sciences, p. 164-259. ISBN 978-0-7020-5317-7. https://doi.org/10.1016/B978-0-7020-5317-7.00003-5

Falk, M., Bernhoft, A., Framstad, T., Salbu, B., Wisløff, H. 2018. Effects of dietary sodium selenite and organic selenium sources on immune and inflammatory responses and selenium deposition in growing pigs. Journal of Trace Elements in Medicine and Biology, vol. 50, р. 527-536. https://doi.org/10.1016/j.jtemb.2018.03.00

GOST 19496-93. 1995. Meat. Method of histological investigation.

GOST 31476. 2012. Pigs for slaughter. Pork carcasses and semi-carcasses. Specifications.

GOST 427-75. 1977. Measuring metal rules. Basic parameters and dimensions. Technical requirements.

Herraez, P., Espinosa de los Monteros, A., Fernandez, A., Edwards, J. F., Sacchini, S., Sierra, E. 2013. Capture myopathy in live-stranded cetaceans. The Veterinary Journal, vol. 196, no. 2, p. 181-188. https://doi.org/10.1016/j.tvjl.2012.09.021

Hollinger, K., Shanely, R. A., Quindry, J. C., Selsby, J. T. 2015. Long-term quercetin dietary enrichment decreases muscle injury in mdx mice. Clinical Nutrition, vol. 34, no. 3, p. 515-522. https://doi.org/10.1016/j. clnu.2014.06.008

Ivanova, S. G., Nakev, J. L., Nikolova, T. I., Vlahova-Vangelova, D. B., Balev, D. K., Dragoev, S. G. 2019. Effect of new liverstock feeds phytonutrients on productivity, carcass composition and meat geniality in pigs. AqriXiv, 18 p. https://doi.org/10.31220/osf.io/jfrvy

Jiang, J., Tang, X., Xue, Y., Lin, G., Xiong, Y. L. 2017. Dietary linseed oil supplemented with organic selenium improved the fatty acid nutritional profile, muscular selenium deposition, water retention, and tenderness of fresh pork. Meat Science, vol. 131, p. 99-106. https://doi.org/10.1016/j.meatsci.2017.03.014

Kijowski, J., Kupinska, E. 2012. Induction of DPM changes in broiler chickens and characteristics of myopathy symptoms. Bull. Vet. Inst. Pulawy, vol. 56, no. 2, p. 217-223, https://doi.org/10.2478/v10213-012-0039-8

Kijowski, J., Konstanczak, M. 2009. Deep pectoral myopathy in broiler chickens. Bull. Vet. Inst. Pulawy, vol. 53, р. 487-491.

Kim, J.-M., Lim, K.-S., Ko, K.-B., Ryu, Y.-C. 2018. Estimation of pork quality in live pigs using biopsied muscle fibre number composition. Meat Science, vol. 137, p. 130-133. https://doi.org/10.1016/j.meatsci.2017.11.020

Kremer, B. T., Stahly, T. S., Sebranek, J. G. 1998. Effect of Dietary Quercetin on Pork Quality. Iowa State University. Swine Research Report, vol. 65. Available at: http://lib.dr.iastate.edu/swinereports_1998/65.

Lee, S. H., Choe, J. H., Cho, Y. M., Jung, K. C., Rhee, M. S., Hong, K. C., Lee, S. K., Ryu, Y. C., Kim, B. C. 2012. The influence of pork quality traits and muscle fiber characteristics on the eating quality of pork from various breeds. Meat Science, vol. 90, no. 2, p. 284-291. https://doi.org/10.1016/j.meatsci.2011.07.012

Lefaucheur, L., Hoffman, R. K., Gerrard, D .E., Okamura, C. S., Rubinstein, N., Kelly, A. 1998. Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibersin pigs. J. Anim. Sci., vol. 76, no. 6, p. 1584-1593. https://doi.org/10.2527/1998.7661584x

Li, J.-G., Zhou, J.-C., Zhao, H., Lei, X.-G., Xia, X.-J., Gao, G., Wang, K.-N. 2011. Enhanced water-holding capacity of meat was associated with increased Sepw1 gene expression in pigs fed selenium-enriched yeast. Meat Science, vol. 87, no. 2, p. 95-100. https://doi.org/10.1016 / j. meatsci.2010.05.019

Listrat, A., Lebret, B., Louveau, I., Astruc, T., Bonnet, M., Lefaucheur, B. Picard, L., Bugeon, J. 2016. How Muscle Structure and Composition Influence Meat and Flesh Quality. Hindawi Publishing Corporatione Scientific World Journal, vol. 2016, 14 p. https://doi.org/10.1155/2016/3182746

Mahan, D. C., Cline, T. R., Richert, B. 1999. Effects of dietary levels of selenium-enriched yeast and sodium selenite as selenium sources fed to growing-finishing pigs on performance, tissue selenium, serum glutathione peroxidase activity, carcass characteristics, and loin quality. J. Anim. Sci., vol. 77, no. 8, p. 2172-2179. https://doi.org/10.2527/1999.7782172x

Mateo, R. D., Spallholz, J. E., Elder, R., Yoon, I., Kim, S. W. 2007. Efficacy of dietary selenium sources on growth and carcass characteristics of growing-finishing pigs fed diets containing high endogenous selenium. J. Anim. Sci., vol. 85, no. 5, p. 1177-1183. https://doi.org/10.2527/jas.2006-067

Minvielle, B., Le Strat, P., Lebret, B., Houix, Y., Boulard, J., Clochefert, N. 2001. Viandes déstructurées: situation dans cinq abattoirs de l’Ouest de la France; facteurs de risqué et proposition d’un modèle; caractérisation colorimétrique, biochimique et histologique. Journées Rech. Porcine en France, vol. 33. р. 95-101. Available at: https://prodinra.inra.fr/record/69602.

Mukai, R., Matsui, N., Fujikura, Y., Matsumoto, N., Hou, D.-X., Kanzaki, N., Shibata, H., Horikawa, M. 2016. Preventive effect of dietary quercetin on disuse muscle atrophy by targeting mitochondria in denervated mice. The Journal of Nutritional Biochemistry, vol. 31, p. 67-76. https://doi.org/10.1016/j.jnutbio.2016.02.001

Nekrasov, R. V., Golovin, A. V., Makhaev, E. A. 2018. Norms of the requirements of dairy cattle and pigs in nutrients. Moscow : RAS, 290 p. ISBN 978-5-906906-77-9.

Petracci, M., Cavani, С. 2012. Muscle Growth and Poultry Meat Quality Issues. Nutrients, vol. 4, no. 1, р. 1-12. https://doi.org/10.3390/nu4010001

Realini, C. E., Vénien, A., Gou, P., Gatellier, P., Pérez-Juan, M., Danon, J. 2013. Characterization of Longissimus thoracis, Semitendinosus and Masseter muscles and relationships with technological quality in pigs. 1. Microscopic analysis of muscles. Meat Science, vol. 94, no. 3, p. 408-416. https://doi.org/10.1016/j.meatsci.2013.03.009

Sales, J., Kotrba, R. 2013. Meat from wild boar (Sus scrofa L.): A review. Meat Science, vol. 94, no. 2, p. 187-201. https://doi.org/10.1016/j.meatsci.2013.01.012

Schubert-Schoppmeyer, A., Fiedler, I., Nurnberg, G., Jonas, L., Ender, K., Maak, S., Rehfeldt, C. 2008. Simulation of giant fibre development in biopsy samples from pig longissimus muscle. Meat Science, vol. 80, no. 4, р. 1297-1303. https://doi.org/10.1016/j.meatsci.2008.06.008

Semenova, A. A., Kuznetsova, T. G., Nasonova, V. V., Nekrasov, R. V., Bogolyubova, N. V. 2019. Myopathy as a destabilizing factor of meat quality formation. Theory and practice of meat processing, vol. 3, no. 4, p. 24-31. https://doi.org/10.21323/2414-438X-2019-4-3-24-31

Vlahova-Vangelova, D. B., Balev, D. K., Ivanova, S. G., Nakev, I. L., Nikolova, T. I., Dragoev, C. G., Gerrard, D. E. 2019. Impruving the оxidative stability of pork by antiaxidan tape phytonutrients. AqriXiv, 27 p. https://doi.org/10.31220/osf.io/gz39p

Zhuravskaya, N. K., Alekhina, L. T., Otryashnikova, L. M. 1985. Study and control of meat and meat product quality. Vestnik Novosibirskogo gosudarstvennogo universiteta, vol. 4, 295 p. https://doi.org/10.31677/2072-6724-2018-49-4-140-147

Published

2020-08-28

How to Cite

Semenova, A., Kuznetsova, T., Nasonova, V., Nekrasov, R., & Bogolubova, N. (2020). Effect of modelled stress and adaptogens on microstructural characteristics of pork from fast-growing hybrid animals. Potravinarstvo Slovak Journal of Food Sciences, 14, 656–663. https://doi.org/10.5219/1388