Transfer efficacy of Escherichia coli O157:H7 between surfaces of green mature tomatoes and common food processing materials

Authors

  • Oleksandr Tokarskyy Ternopil National Medical University, International Students’ Faculty, Department of Medical Biochemistry, Maidan Voli 1, 46001, Ternopil, Ukraine, Tel: +380964102536 https://orcid.org/0000-0001-6279-1803
  • Mykhaylo Korda Ternopil National Medical University, Department of Medical Biochemistry, Maidan Voli 1, 46001, Ternopil, Ukraine, Tel: +380352524492 https://orcid.org/0000-0002-6066-5165
  • Halyna Lukyantseva National University of Ukraine on Physical Education and Sport, Biomedical Disciplines Department, Fizkultury street 1, 03150, Kyiv-150, Ukraine, Tel: +380975777765 https://orcid.org/0000-0002-8054-0108

DOI:

https://doi.org/10.5219/1408

Keywords:

tomatoes, steel, vinyl conveyor belt, plastic, E. coli O157:H7, survival, transfer

Abstract

The objectives of this study were: a) to evaluate E. coli O157:H7 survival on green mature tomatoes and squares of common food processing materials – stainless steel, plastic (HDPE), and vinyl conveyor belt (PVC) – post-drying, stored at 25 ºC in the humidified environment for four days; b) to determine pathogen transfer rates (wet, 90 minutes, or 24-hours drying post-inoculation), from inoculated tomato surfaces to uninoculated steel, plastic, and vinyl conveyor belt squares and conversely. It was shown that E. coli O157:H7 did not survive well on the surface of tomatoes, resulting in a decline from 5.3 log10 CFU.mL-1 90 minutes post-drying to 1.4 log10 CFU.mL-1 on day 4. Similarly, the pathogen did not survive well on the surface of food processing squares, with numbers declining over 4 days from 4.04, 4.44, and 4.19 CFU.mL-1 of rinsate 90 minutes squares post-drying to 0.72, 0.50, 0.83  log10 CFU.mL-1, which is close to the detection limit, for the steel, vinyl belt, and plastic, respectively. Successful cross-contamination between tomatoes and food processing surfaces was achieved during wet transfer; while transfer after 90 minutes inoculum post-drying and
24 hours were less successful. This can be explained by both lack of liquid media with suspended bacteria for transfer and fast pathogen die-off after desiccation. Dry transfers, as shown by the percentage of “positive” for pathogen presence tomatoes and squares, as well as bacterial counts, were more successful from tomatoes to squares, but not conversely. Special concern raised vinyl conveyor belt, where the surface picked up the most pathogen cells from the surface of tomatoes, resulting in 100% positive during 90 minute-dry transfers, followed by plastic (66.7% positive) and steel (55.6% positive). To summarize, we presented data on the possibility of cross-contamination between mature green tomatoes and common food processing surfaces, which may be interesting for the processors for risk evaluation.

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References

Beuchat, R. Y., Ryu, J. H., 1997. Produce handling and processing practices. Emerging Infectious Disease, vol. 3, p. 439-465. https://doi.org/10.3201/eid0304.970407

Brar, P. K., Danyluk, M. D. 2013. Salmonella transfer potential during hand harvesting of tomatoes under laboratory conditions. Journal of Food Protection, vol. 76, no. 8, p. 1342-1349. https://doi.org/10.4315/0362-028X.JFP-13-048

Buchholz, A. L., Davidson, G. R., Marks, B. P., Todd, E. C. D., Ryser, E. T. 2012. Transfer of Escherichia coli O157:H7 from equipment surfaces to fresh-cut leafy greens during processing in a model pilot-plant production line with sanitizer-free water. Journal of Food Protection, vol. 75, no. 11, p. 1920-1929. https://doi.org/10.4315/0362-028X.JFP-11-558

Burnett, S. L., Chen, J., Beuchat, L. R. 2000. Attachment of Escherichia coli O157:H7 to the surfaces and internal structures of apples as detected by confocal scanning laser microscopy. Applied and Environmental Microbiology, vol. 66, no. 11, p. 4679-4687. https://doi.org/10.1128/aem.66.11.4679-4687.2000

CDC. 2002. Centers for Disease Control and Prevention. 2002. Outbreak of Salmonella serotype Javiana infections -Orlando, Florida, June 2002. Morbidity and Mortality Weekly Report, vol. 51, p. 683-684.

Croby, R., Lanni, V., Kistler, V., Dato, V., Weltman, A., Yozviak, C., Waller, K., Nalluswami, K., Moll, M., Lockett, J., Montgomery, S., Lyuch, M., Braden, C., Gupta, S. K., Dubois, A. 2005. Outbreaks of Salmonella infections associated with eating Roma tomatoes-United States and Canada, 2004. Morbidity and Mortality Weekly Report, vol. 54, p. 325-328.

Cummings, K., Barrett, E., Mohle-Boetani, J. C., Brooks, J. T., Farrar, J, Hunt, T., Fiore, A., Komatsu, K., Werner, S. B., Slutsker, L. 2001. A multistate outbreak of Salmonella enterica serotype Baildon associated with domestic raw tomatoes. Emerging Infectious Disease, vol. 7, no.6, p. 1046-1048. https://doi.org/10.3201/eid0706.010625

Daş, E., Gürakan, G. C., Bayindirli, A. 2006. Effect of controlled atmosphere storage, modified atmosphere packaging and gaseous ozone treatment on the survival of Salmonella Enteritidis on cherry tomatoes. Food Microbiology, vol. 23, no. 5, p. 430-438. https://doi.org/10.1016/j.fm.2005.08.002

Hirai, Y. 1991. Survival of bacteria under dry conditions; from a viewpoint of nosocomial infection. Journal of Hospital Infection, vol. 19, no. 3, p. 191-200. https://doi.org/10.1016/0195-6701(91)90223-U

Jensen, D. A., Friedrich, L. M., Harris, L. J., Danyluk, M. D., Schaffner, D. W. 2013. Quantifying transfer rates of Salmonella and Escherichia coli O157:H7 between fresh-cut produce and common kitchen surfaces. Journal of Food Protection, vol. 76, no. 9, p. 1530-1538. https://doi.org/10.4315/0362-028X.JFP-13-098

Jiang, X., Doyle, M. P. 1999. Fate of Escherichia coli O157:H7 and Salmonella enteritidis on currency. Journal of Food Protection, vol. 62, no. 7, p. 805-807. https://doi.org/10.4315/0362-028x-62.7.805

Kramer, A., Schwebke, I., Kampf, G. 2006. How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infectious Diseases, vol. 6, 8 p. https://doi.org/10.1186/1471-2334-6-130

Kusumaningrum, H. D., Riboldi, G., Hazeleger, W. C., Beumer, R. R. 2003. Survival of foodborne pathogens on stainless steel surfaces and cross-contamination to foods. International Journal of Food Microbiology, vol. 85, no. 3, p. 227-236. https://doi.org/10.1016/S0168-1605(02)00540-8

Lang, M. M., Harris, L. J., Beuchat, L. R. 2004. Evaluation of inoculation method and inoculum drying time for their effects on survival and efficiency of recovery of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes inoculated on the surface of tomatoes. Journal of Food Protection, vol. 67, no. 4, p. 732-741. https://doi.org/10.4315/0362-028X-67.4.732

Møretrø, T., Heir, E., Mo, K. R., Habimana, O., Abdelgani, A., Langsrud, S. 2010. Factors affecting survival of Shigatoxin-producing Escherichia coli on abiotic surfaces. International Journal of Food Microbiology, vol. 138, no. 1-2, p. 71-77. https://doi.org/10.1016/j.ijfoodmicro.2010.01.002

Schilling, M. W., Yoon, Y., Tokarskyy, O., Pham, A. J., Williams, R. C., Marshall, D. L. 2009. Effects of ionizing irradiation and hydrostatic pressure on Escherichia coli O157:H7 inactivation, chemical composition, and sensory acceptability of ground beef patties. Meat Science, vol. 81, no. 4, p. 705-710. https://doi.org/10.1016/j.meatsci.2008.10.023

Shi, X., Namvar, A., Kostrzynska, M., Hora, R., Warriner, K. 2007. Persistence and growth of different Salmonella serovars on pre- and postharvest tomatoes. Journal of Food Protection, vol. 70, no. 12, p. 2725-2731. https://doi.org/10.4315/0362-028X-70.12.2725

Soares, V. M., Pereira, J. G., Viana, C., Izidoro, T. B., dos Santos Bersot, L., de Almeida Nogueira Pinto, J. P. 2012. Transfer of Salmonella Enteritidis to four types of surfaces after cleaning procedures and cross-contamination to tomatoes. Food Microbiology, vol. 30, no. 2, p. 453-456. https://doi.org/10.1016/j.fm.2011.12.028

Todd-Searle, J., Friedrich, L. M., Oni, R. A., Shenge, K., LeJeune, J. T., Micallef, S. A., Danyluk, M. D., Schaffner, D. W. 2020. Quantification of Salmonella enterica transfer between tomatoes, soil, and plastic mulch. International Journal of Food Microbiology, vol. 316, 9 p. https://doi.org/10.1016/j.ijfoodmicro.2019.108480

Tokarskyy, O., De, J., Fatica, M. K., Brecht, J., Schneider, K. R. 2018. Survival of Escherichia coli O157:H7 and Salmonella on bruised and unbruised tomatoes from three ripeness stages at two temperatures. Journal of Food Protection, vol. 81, no. 12, p. 2028-2033. https://doi.org/10.4315/0362-028X.JFP-18-220

Tokarskyy, O., Korda, M. 2019a. Microbiological comparison of visibly dirty and visibly clean mature green tomatoes before and after treatments with deionized water or chlorine in model overhead spray brush roller system. Potravinarstvo Slovak Journal of Food Sciences, vol. 13, no. 1, p. 779-783. https://doi.org/10.5219/1178

Tokarskyy, O., Korda, M. 2019b. Influence of suspension liquid total solids on E. coli O157:H7 survival and transfer efficacy between green tomatoes and cardboard. Potravinarstvo Slovak Journal of Food Sciences, vol. 13, no. 1, p. 941-949. https://doi.org/10.5219/1210

Tokarskyy, O., Marshall, D. L., Schilling, M. W., Willeford, K. O. 2009. Comparison of methods to verify end point cooking temperature of channel catfish (ictalurus punctatus) fillets. Journal of Muscle Foods, vol. 20, no. 3., p. 325-340. https://doi.org/10.1111/j.1745-4573.2009.00151.x

Tokarskyy, O., Schneider, K. 2019. Influence of temperature, humidity, and diluent type on survival of Salmonella spp. on the surface of raw tomatoes. Potravinarstvo Slovak Journal of Food Sciences, vol. 13, no. 1, p. 325-330. https://doi.org/10.5219/1121

USDA-ERS. 2016. United States Department of Agriculture, Economic Research Service. Loss-adjusted food availability data. Available at: https://www.ers.usda.gov/data-products/chart-gallery/gallery/chart-detail/?chartId=58340

Wei, C. I., Huang, T. S., Kim, J. M., Lin, W. F., Tamplin, M. L., Bartz, J. A. 1995. Growth and survival of Salmonella montevideo on tomatoes and disinfection with chlorinated water. Journal of Food Protection, vol. 58, no. 8, p. 829-838. https://doi.org/10.4315/0362-028X-58.8.829

Zhuang, R. Y., Beuchat, L. R., Angulo, F. J. 1995. Fate of Salmonella Montevideo on and in raw tomatoes as affected by temperature and treatment with chlorine. Applied and Environmental Microbiology, vol. 61, no. 6, p. 2127-2131. https://doi.org/10.1128/AEM.61.6.2127-2131.1995

Published

2020-10-28

How to Cite

Tokarskyy, O., Korda, M., & Lukyantseva, H. (2020). Transfer efficacy of Escherichia coli O157:H7 between surfaces of green mature tomatoes and common food processing materials. Potravinarstvo Slovak Journal of Food Sciences, 14, 1027–1034. https://doi.org/10.5219/1408