Presence of S. aureus and Enterococcus spp. in goat´s cheese and their antibiotic resistance

Authors

  • Lucia Poľáková Department of Food Hygiene and Technology, Institute of Milk Hygiene and Technology, University of Veterinary Medicine and Pharmacy, Komenského 71, 041 81 Košice
  • Eva Dudrikova Department of Food Hygiene and Technology, Institute of Milk Hygiene and Technology, University of Veterinary Medicine and Pharmacy, Komenského 71, 041 81 Košice
  • Juraj Gallo Department of Food Hygiene and Technology, Institute of Milk Hygiene and Technology, University of Veterinary Medicine and Pharmacy, Komenského 71, 041 81 Košice

DOI:

https://doi.org/10.5219/144

Keywords:

Staphylococcus spp., Enterococcus spp., antibiotic resistance, goat´s cheese

Abstract

The aim of this study was to isolate strains of Staphylococcus spp. and Enterococcus spp. from artisanal goat`s cheese during 30 days of ripening, and to determine their antibiotic resistance. Of the total received isolates, 53 (72.60 %) isolates were confirmed  as Staphylococcus spp., from which was only 5.66% (n = 3) detected as S. aureus; and 91 (82.72 %) isolates were confirmed as Enterococcus spp. by multiplex PCR. Antibiotic resistance was tested by disc diffusion method. The 69.81% (n = 37) of staphylococci and 56.04% (n = 51) Enterococcus spp. as resistant were detected to one and more antibiotic drugs. Staphylococci were resistant to penicillin 69.81%, ampicillin 49.06%, oxacillin 39.62%, erythromycin 20.75%, and gentamycin 16.68 %. The highest resistance of enterococci was to cephalothin 42.86% and clindamycin 23.08%.

Downloads

Download data is not yet available.

References

ARAYA, M. - DAVIDOVICH, G., CHAVES, C. 2005. Identification of Enterococcus sp. isolated from raw milk samples coming from the metropolitan area of Costa Rica and evaluation of its antibotic sensibility pattern. In Archivos Latinoamericanos de Nutricio, vol. 55, 2005, no. 2, p. 161-166. PMid:16335226

BERESFORD, T. P. - FITZIMONS, N. A. - BRENNAN, N. L. - COGAN T. M. 2001. Recent advances in cheese microbiology. In Microbiol. Int. Dairy J., vol. 11, 2001, no. 4-7, p. 259-274. http://www.sciencedirect.com/scidirimg/clear.gifhttps://doi.org/10.1016/S0958-6946(01)00056-5

BHARDWAJ, A. - MALIK, R. K. - CHAUHAN, P. 2008. Functional and safety aspects of enterococci in dairy foods. In Indian J. Microbiol., vol. 48, 2008, no. 3. p. 317-325. https://doi.org/10.1007/s12088-008-0041-2

BOCKELMANN, W. 2002. Development of defined surface starter cultures for the ripening of smear cheeses. In International Dairy Journal, vol. 12, 2002, no. 2-3, p. 123-131. https://doi.org/10.1016/S0958-6946(01)00152-2

BOCKELMANN, W., HOPPE-SEYLER, T. 2001. The surface flora of bacterial smear-ripened cheeses from cow's and goat's milk. In International Dairy Journal, vol. 11, 2001, no 4-7 p. 307-314. https://doi.org/10.1016/S0958-6946(01)00060-7

CLSI (Clinical and Laboratory Standards Institute): Performance standards for antimicrobial susceptibility testing. 2006. In 16th Informational Supplement. CLSI document M100-S16. Wayne, PA, USA, 2006, 92p.

CORTÉS, C. - De la FUENTE, R. - CONTRERAS, A. - SÁNCHEZ, A. - CORRALES, J. C. - RUIZ-SANTA-QUITERIA, J. A. - ORDEN, J. A. 2006. Occurrence and preliminary study of antimicrobial resistance of enterococci isolated from dairy goats in Spain. In Internat. J. Food Microbiol., vol. 110, 2006, no 1, p. 100-103. https://doi.org/10.1016/j.ijfoodmicro.2006.01.033 PMid:16682094

CUPÁKOVÁ, Š. - DUŠKOVÁ, M. - JANŠTOVÁ, B. - NECIDOVÁ, L. - KARPÍŠKOVÁ, R. 2010. Druhové zastoupení a rezistence enterokoků izolovaných ze syrového a pasterovaného ovčího mléka. In Celostátní přehlídky sýrů 2010. Výsledky přehlídek a sborník přednášek konference Mléko a sýry. Praha leden 2010, s. 242-244. ISBN 978-80-7080-760-6.

ČANIGOVÁ, M. - KREBS-ARTIMOVÁ. A. - KROČKO, M. - DUCKOVÁ, V. 2010. Antibiotická rezistencia enterokokov izolovaných z rôznych zdrojov v prvovýrobe mlieka. In Celostátní přehlídky sýrů 2010 Výsledky přehlídek a sborník přednášek konference Mléko a sýry. Praha leden 2010,p. 237-241, ISBN 978-80-7080-760-6.

DUDRIKOVÁ, E. - PILIPČINEC, E. - BURDOVÁ. O. 1998. Stafylokoky izolované z ovčieho mlieka iné ako Staphylococcus aures. In: Zborník XIX. Hygiena alimetorum, ŠVS SR a UVL, Košice, 1998, 66-67.

DSMZ, 2009. Nomenclature up-to-date: Approved lists, validation lists. Deutsche Sammlung für Mikroorganismen und Zellkulturen. Retriewed from the web: .

DSMZ, 2010. Bacterial nomenclature up-to-date (Approved lists, validation lists). Deutsche Sammlung für Mikroorganismen und Zellkulturen GmbH Braunschweig, Germany April 2010. Retriewed from the web: .

EFSA, 2007. EFSA public consultation on the Qualified Presumption of Safety (QPS) approach for the safety assessment of microorganisms deliberately added to food and feed. European Food Safety Authority. Retriewed from the web: .

FABIANOVÁ, J., DUCKOVÁ, V., ČANIGOVÁ, M., KROČKO, M.: Výskyt enterokokov v kravskom mlieku a ich rezistencia na antibiotiká. In Potravinarstvo. ISSN 1337-0960, vol. 4, 2010, no 2, p 17-21. https://doi.org/10.5219/45

FARIA, C.- VAZ-MOREIRA, I.- SERAPICOS, E.- NUNES, O. C.- MANAIA, C. M. 2009. Antibiotic resistance in coagulase negative staphylococci isolated from wastewater and drinking water. In Sci. Total Environ., vol. 407, 2009, no. 12, p. 3876-3882, ISSN: 0048-9697. http://www.sciencedirect.com/scidirimg/clear.gifhttps://doi.org/10.1016/j.scitotenv.2009.02.034 PMid:19324394

FONTANA, C., CAPPA, F., REBECCHI, A., COCCONCELLI, P. S. 2010. Surface microbiota analysis of Talleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses. In International Journal of Food Microbiology, vol. 138, 2010, no. 3, p. 205-211.https://doi.org/10.1016/j.ijfoodmicro.2010.01.017 PMid:20167385

GELSOMINO, R. - VANCANNEYT, M. - COGAN, T. M. - CONDON, S. - SWINGS, J. 2002. Source of enterococci in a farmhouse raw-milk cheese. In Applied and Environmental Microbiology, vol. 68, 2002, p. 3560-3565. https://doi.org/10.1128/AEM.68.7.3560-3565.2002 PMid:12089042

GIRAFFA, G. 2002. Enterococci from foods. In FEMS Microbiology Reviews, vol. 26, 2002, no. 2, p. 163-171. https://doi.org/10.1111/j.1574-6976.2002.tb00608.x PMid:12069881

GÖRNER, F. - VALÍK, Ľ. 2004. In Aplikovaná mikrobiológia požívatín. Malé Centrum, Bratislava, ISBN : 80-967064-9-7, 528 p.

IRLINGER, F. 2008. Safety assessment of dairy microorganisms: coagulase-negative staphylococci. In International Journal of Food Microbiology, vol. 126, 2008, no. 3, p. 302-310. https://doi.org/10.1016/j.ijfoodmicro.2007.08.016 PMid:17869361

JAY, J. M. - LOESSNER, M. J. - GOLDEN, D. A. 2005. Moderm Food Microbiology. Springer Science, NY USA, 790 p. ISBN: 0-387-23180-3.

JORGENSEN, H. J.- MØRK, T.- RØRVIK, L. M. 2005. The ocurrence of Staphylococcus aureus on a farm with small-scale production of raw milk cheese. In Journal of Dairy Science, vol. 88, 2005, no. 11, 3810-3817. https://doi.org/10.3168/jds.S0022-0302(05)73066-6

KE, D. - PICARD, F. J. - MARTINEAU, F. - MÉNARD, C. H. - ROY, P. H. - OUELLETTE, M. - BERGERON, M. G. 1999. Development of a PCR assay for rapid detection of enterococci. In J. Clin. Microbiol., vol. 37, 1999, no. 11, p. 3497-3503. PMid:10523541

MARTINEAU, F. - PICARD, F. J. - ROY, P. H. - OULLETTE, M. - BERGERON, M. G. 1996. Species-specific and ubiquitous DNA-based assays for rapid identification of Staphylococcus epidermidis. In J. Clin. Microbiol., vol. 34, 1996, no. 12, p. 2888-2893. PMID: 8940417

MARTINEAU, F. - PICARD, F. J. - ROY, P. H .- OUELLETTE, M. - BERGERON, M. G. 1998. Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus. In J. Clin. Microbiol., vol. 36, 1998, p. 618-623. PMid:9508283

PERRETEN, V. - SCHWARZ, F. - CRESTA, L. - BOEGLIN, M. - DASEN, G. - TEUBER, M. 1997. Antibiotic resistance spread in food. In Nature, vol. 389, 1997, p. 801-802, ISSN 0028-0836. https://doi.org/10.1038/39767

PETRÍK, P .- TRÁVNIČEK, M. - ČÍŽEK, M., FOTTA, M. - MARDZINOVÁ, S. 2001. Identifikácia koaguláza negatívnych stafylokov izolovaných zo surového mlieka oviec a kôz. In: Zborník Atuálne problémy epizootológie a infektológie, november 2001, 142-146.

PILIPČINCOVÁ, I. - BHIDE, M .- DUDRIKOVÁ, E .- TRÁVNIČEK, M. 2010. Genotypic characterization of coagulase-negative staphylococci isolated from Sheep milk in Slovakia. In Acta Vet., Brno, vol 79, 2010, no. 4, p. 269-275. ISSN 0001-7213 (printed), ISSN 1801-7576 (electronic).

PLACE, R. B. - HIESTAND, D. - BURRI, S. - M. TEUBER, M. 2002. Staphylococcus succinus subsp. casei subsp. nov., a dominant isolate from a surface ripened cheese. In Systematic and Applied Microbiology, vol. 25, 2002, no. 3, p. 353-359. https://doi.org/10.1078/0723-2020-00130PMid:12421073

PLACE, R. B. - HIESTAND, D. - GALLMANN, H. R. - TEUBER, M. 2003. Staphylococcus equorum subsp. linens, subsp. nov., a starter culture component for surface ripened semi-hard cheeses, Systematic and Applied Microbiology, vol. 26, 2003, no. 1, 30-37.https://doi.org/10.1078/072320203322337281 PMid:12747407

POĽÁKOVÁ, L. - GALLO,J. - DUDRIKOVÁ, E. - FRIČOVÁ, L. - CUPÁKOVÁ,Š. - ŠPILOVSKÁ, S. 2010. Sledovanie antibiotickej citlivosti Enterococcus spp. In III. Vedecká konferencia stretnutie mladých vedeckých pracovníkov v potravinárstve. Gabčíkovo, 2.-3. december 2010, p. 285-291, ISBN 978-80-227-3411-0.

RESCH, M. - NAGEL, V. - HERTEL, C. 2008. Antibiotic resistance of coagulase-negative staphylococci associated with food and used in starter cultures. In Int. J. Food Microbiol., vol. 127, 2008, p. 99-104, ISSN: 0168-1605. https://doi.org/10.1016/j.ijfoodmicro.2008.06.013 PMid:18625535

SAMPIMONETAL, O. C. - ZADOKS, R. N. - DEVLIEGHER, S. - SUPRE, K.. - HAESEBROUCK, F. - BARKEMA, H. W. - SOL, J. - LAM, T. J. G. M. 2009. Performance of API Staph ID 32 and Staph-Zym for identification of coagulase-negative staphylococci isolated from bovine milk samples. In Vet. Microbiol., vol. 136, 2009, p. 300-305, ISSN 0378-1135. http://www.sciencedirect.com/scidirimg/clear.gifhttps://doi.org/10.1016/j.vetmic.2008.11.004 PMid:19110383

SEITTER (NÉE RESCH), M. - CHRISTIANE NERZ, CH. - ROSENSTEIN, R. - GÖTZ, F. - HERTEL, CH. 2011. DNA microarray based detection of genes involved in safety and technologically relevant properties of food associated coagulase-negative staphylococci. In Int. J. Food Microbiol., vol. 145, 2011, no. 2-3, p. 449-458. https://doi.org/10.1016/j.ijfoodmicro.2011.01.021 PMid:21329998

SOARES, J. C. - MARQUES, M. R. - TAVARIA, F. K.- PEREIRA, J. O. - MALCATA, F. X. - MANUELA M. PINTADO, M. M. in press. Biodiversity and characterization of Staphylococcus species isolated from a small manufacturing dairy plant in Portugal. In Int. J. Food Microbiol., vol. 146, 2011, no. 2. http://www.sciencedirect.com/scidirimg/clear.gifhttps://doi.org/10.1016/j.ijfoodmicro.2011.02.008 PMid:21411163

STROMMENGER, B .- KETTLITZ, C. - WERNER, G. - WITTE, W. 2003: Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus. In J. Clin. Microbiol., vol. 43, 2003, p. 4089-4094, Online ISSN: 1098-660X, Print ISSN: 0095-1137. https://doi.org/10.1128/JCM.41.9.4089-4094.2003 PMid:12958230

STN ISO 6887: 1997. In Mikrobiológia. Všeobecné pokyny na prípravu riedení pri mikrobiologickom skúšaní.

ŠUSTÁČKOVÁ, A. - NÁPRAVNÍKOVÁ, E. - NAVRÁTILOVÁ, P. 2003. Potraviny živočišného původu jako vektor rezistence k antibakteriálním látkam. In Zb. Aktuálne problémy riešené v agrokomplexe. Nitra, SPU, 2003, p. 328-342, ISBN 80-8069-295-5.

VASIĽ, M. - ELEČKO, J. - FOTTA, M. - JACKOVÁ, A. - KALINÁČOVÁ, V. - SIKLENKA, P. 2001. (Záverečná správa) Výskumný ústav veterinárnej medicíny Košice, 2001, 38 p.

VASIĽ, M. - ELEČKO, J. - FOTTA, M. 2007. Výskyt baktérií Staphylococcus spp. v mlieku prežúvavcov. In: Zborník Hygiena alimetorum XXVIII, Štrbské pleso-Vysoké Tatry, 2- 4 mája. 2007, 111-115, ISBN 978-80-8077-055-6.

WILLIAMS, A. G. - WITHERS, S. E. 2010. Microbiological characterisation of artisanal farmhouse cheeses manufactured in Scotland. In Int. J. Dairy Technology, vol. 3, 2010, p. 305-476. https://doi.org/10.1111/j.1471-0307.2010.00596.x

WITTE, W. - CUNY, C. - KLARE, I. - NUBEL, U. - STROMMENGER, B. - WERNER, G. 2008. Emergence and spread of antibiotic-resistant Gram-positive bacterial pathogens. In Int. J. Med. Microbiol., vol. 298, 2008, no. 5-6, p. 365-377, ISSN 1438-4221. http://www.sciencedirect.com/scidirimg/clear.gifhttps://doi.org/10.1016/j.ijmm.2007.10.005 PMid:18325835

ZADOKS, R. N. - WATTS, J. L. 2009. Species identification of coagulase-negative staphylococci: genotyping is superior to phenotyping. In Vet. Microbiol., vol. 134, 2009, p. 20-28, ISSN 0378-1135. http://www.sciencedirect.com/scidirimg/clear.gifhttps://doi.org/10.1016/j.vetmic.2008.09.012 PMid:18930607

ZELL, C. - RESCH, M. - ROSENSTEIN, R. - ALBRECHT, T. - HERTEL, C. - GOTZ, F. 2008. Characterization of toxin production of coagulase-negative staphylococci isolated from food and starter cultures. In Int. J. Food Microbiol., vol. 127, 2008, p. 246-25, ISSN 0168-1605.https://doi.org/10.1016/j.ijfoodmicro.2008.07.016 PMid:18752861

Downloads

Published

2011-06-25

How to Cite

Poľáková, L. ., Dudrikova, E. ., & Gallo, J. . (2011). Presence of S. aureus and Enterococcus spp. in goat´s cheese and their antibiotic resistance. Potravinarstvo Slovak Journal of Food Sciences, 5(3), 33–37. https://doi.org/10.5219/144

Similar Articles

You may also start an advanced similarity search for this article.