Optimalisation of species identification of common carp (Cyprinus carpio) using SYBR® green I real-time PCRmethod

Authors

  • Pavol Bajzí­k Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Radoslav Židek Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Golian Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Ľubomí­r Belej Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Čapla Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Lenka Maršálková Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Ondrej Revák Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/154

Keywords:

species identification, SYBR® Green I. Real-Time PCR, Cyprinus carpio

Abstract

European Union Member States, together with a number of countries around the world, places great emphasis on ensuring the protection of consumers as a potential food allergic from food allergies. to inform consumers that their product may contain any of the risk of allergenic ingredients. For species identification of fish and fish products as a potential food allergens are used by many analytical methods as well as their authentication. We are in our work applied the method of SYBR® Green I. Real-Time PCR. We focused on pre-designed molecular - genetic marker of common carp (Cyprinus carpio), which comes from the mtDNA control D - loop area. We analyzed its presence in DNA isolates from the 5 kinds of freshwater fish, diluted to 10 % concentration. Results of using the optimized SYBR ® Green I Real-Time PCR method for species identification common carp (Cyprinus carpio) indicate to its suitability.

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Published

2011-07-03

How to Cite

Bajzí­k, P. ., Židek, R. ., Golian, J. ., Belej, Ľubomí­r ., Čapla, J. ., Maršálková, L. ., & Revák, O. . (2011). Optimalisation of species identification of common carp (Cyprinus carpio) using SYBR® green I real-time PCRmethod. Potravinarstvo Slovak Journal of Food Sciences, 5(3), 1–5. https://doi.org/10.5219/154

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