Optimalisation of species identification of common carp (Cyprinus carpio) using SYBR® green I real-time PCRmethod
Keywords:species identification, SYBRÂ® Green I. Real-Time PCR, Cyprinus carpio
European Union Member States, together with a number of countries around the world, places great emphasis on ensuring the protection of consumers as a potential food allergic from food allergies. to inform consumers that their product may contain any of the risk of allergenic ingredients. For species identification of fish and fish products as a potential food allergens are used by many analytical methods as well as their authentication. We are in our work applied the method of SYBR® Green I. Real-Time PCR. We focused on pre-designed molecular - genetic marker of common carp (Cyprinus carpio), which comes from the mtDNA control D - loop area. We analyzed its presence in DNA isolates from the 5 kinds of freshwater fish, diluted to 10 % concentration. Results of using the optimized SYBR ® Green I Real-Time PCR method for species identification common carp (Cyprinus carpio) indicate to its suitability.
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