The effect of the probiotics Bacillus subtilis (PB6) on the selected indicators of the table eggs quality, fat and cholesterol

Authors

  • Mária Angelovičová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra
  • Ebrahim Alfaig Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra
  • Martin Král Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra
  • Jana Tkáčová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/271

Keywords:

Bacillus subtilis, egg, feed, weight, fat, cholesterol

Abstract

The aim of this study was to determine the effect of dietary probiotics Bacillus subtilis (PB6) on egg weigh, egg mass weigh, egg fat content and cholesterol content in egg yolk in laying hens ISA Brown during two experiments. The probiotics where supplied to the laying hens for 42 days as preparation period before eggs samples collection. The eggs samples were collected during 6 days for the 1st and 2nd experiments after the hens reached the age of 34 and 61 weeks, respectively.  A total of 36 ISA Brown laying hens were divided into 2 treatment groups. Control group laying hens were fed a basal diet with no probiotic added. In group Bacillus subtilis, the basal diet was supplemented with the bacteria Bacillus subtilis (PB6) at 1 g/kg, min. 2.3*108 cfu/g. Dietary treatments did not significantly affect the egg weigh, internal egg content weigh, cholesterol content expressed by g/100 g of egg yolk.  Bacillus subtilis (PB6) supplementation significantly (p < 0.05) increased the fat content in the internal egg content and cholesterol content in egg yolk expressed as g/pc. 

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References

Abdulrahim, S. M., Haddadin, S. Y., Hashlamoun, E. A., Robinson, R. K. 1996. The influence of Lactobacillus acidophilus and bacitracin on layer performance of chickens and cholesterol content of plasma and egg yolk. British Poultry Science, vol. 37, p. 341-346. https://doi.org/ 10.1080/00071669608417865 PMid:8773843

Arpášová, H., Kačániová, M., Haščík, P., Šidlová, V. 2012. Effect of selected feed additives on internal quality parameters of table eggs. Potravinarstvo, vol. 6, no. 4, p. 52-61. https://doi.org/10.5219/235

Asli, M. M., Hosseini, S. A., Lotfollahian, H., Shariatmadari, F. 2007. Effect of probiotics, yeast, vitamin E and vitamin C supplements on performance and immune response of laying hen during high environmental temperature. International Journal of Poultry Science, vol. 6, no. 12, p. 895-900. https://doi.org/10.3923/ijps. 2007.895.900

Bailey, J. S. 1987. Factors affecting microbial competitive exclusion in poultry overview-outstanding symposia in food science and technology. Food Technology, p. 88-92.

Capcarová, M., Chmelničná, L., Kolesarová, A., Massanyi, P., Kovačík, J. 2010. Effect of Enterococcus faecium M 74 strain on selected blood and production parameters of laying hens. British Poultry Science, vol. 51, no. 5, p. 614-620. https://doi.org/10.1080/00071668.2010.513961 PMid:21058064

Coates, M. E., Fuller, R. 1977. The genotobiotic animal in the study of gut microbiology. In Clarke , R. T. J., Bauchop, T.: Microbial Ecology of the Gut. Academic Press : London, p. 311-346.

Dunham, H. J., William, C., Edens, F. W., Casas, I. A., Dobrogosz, W. J. 1993. Lactobacillus reuteri immunomodulation of stressor associated disease in newly hatched chickens and turkeys. Poultry Science, vol. 72 (Suppl. 1), p. 103.

Earl, A. M., Losick, R., Kolter, R. 2007. Bacillus subtilis genome diversity. Journal of Bacteriology, vol. 189, p. 1163-1170. https://doi.org/10.1128/JB.01343-06 PMid:17114265

Fukushima, M., Nakano, M. 1995. The effect of a probiotic on faecal and liver lipid classes in rats. British Journal Nutrition, vol. 73, p. 701-710. https://doi.org/10.1079/ BJN19950074 PMid:7626589

Fuller, R. 1989. Probiotics in man and animals. A review. Journal of Applied Bacteriology, vol. 66, p. 365-378. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x PMid:2666378

Gajdoš, M., Cibulová, Ľ., Krivošíková, Z. 2004. Rosuvastatín: nový inhibítor 3-hydroxy-3-methylglutaryl koenzým a reduktázy. In Via Practica, vol. 1, p. 21-22.

Gallazzi, D., Giardini, A., Mangiagalli, M. G., Marelli, S., Ferrazzi, V., Orsi, C., Cavalchini, L. G. 2008. Effects of Lactobacillus addophilus D2/CSL on laying hen performance. Italian Journal of Animal Science, vol. 7, 2008, no. 1, p. 27-37.

Gilliland, S. E., Kim, S. H. 1984. Effect of viable starter culture bacteria in yogurt on lactose utilization in humans. Journal of Dairy Science, vol. 97, p. 1-6. https://doi.org/10.3168/jds.S0022-0302(84)81260-6 PMid:6707296

Gilliland, S. E., Nelson, C. R. Maxwell, C. 1985. Assimilation of cholesterol by Lactobacillus acidophilus bacteria. Applied and Environmental Microbiology, vol. 49, p. 377-381. PMid:3920964

Haddadin, M. S. Y., Abdulrahim, S. M., Hashlamoun Nahashon, E. A. R. S. N., Nakaue, H. S., Mirosh, I. W., Robinson, R. K. 1996. The effects of Lactobacillus acidophilus on the production and chemical composition of hen's eggs. Poultry

Science, vol. 75, p. 491-494. https://doi.org/ 10.3382/ps.0750491 PMid:8786938

Ingr, I., Simeonová, J. 1983. Rýchle stanovení cholesterolu ve vaječném žloutku Bio-la-testem. Veterinary Medicine - Czech, vol. 28, p. 97-104. PMid:6405530

Jin, L. Z., Ho, Y. W., Abdullah, N., Jalaludin, S. 1996. Influence of dried Bacillus subtilis and lactobacilli cultures on intestinal microflora and performance in broilers. Asian-australas. Journal Animal Science, vol. 9, p. 397-404.

Jin, L. Z., Ho, Y. W., Abdullah, N., Jalaludin, S. 1997. Effect of adherent Lactobacillus culture on growth, weight of organs and intestinalmicroflora and volatile fatty acids in broilers. Animal Feed Science Technology, vol. 70, p. 197-209. https://doi.org/10.1016/S0377-8401(97)00080-1

Jin, L. Z., Ho, Y. W., Abdullah, N., Jalaludin, S. 1998. Growth performance, intestinal microbial populations and serum cholesterol of broilers diets containing Lactobacillus cultures. Poultry Science, vol. 77, p. 1259-1265. PMid:9733111

Kačániová, M., Bobček, R., Kmeť, V., Angelovičová, M. 2005. Kŕmne doplnky ako náhrada antibiotík a ďalšie aplikácie. Nitra : SPU, 2005, 78 p. ISBN 80-8069-589-X.

Kačániová, M., Nováková, I., Haščík, P. 2011. Význam probiotík a ich význam na mikroflóru gastrointestinálneho traktu hydiny. Nitra : SPU, 181 p. ISBN 978-80-552-0655-4.

Kalavathy, R., Abdullah, N., Jalaludin, S. Ho, Y. W. 2003. Effects of Lactobacillus cultures on growth performance, abdominal fat deposition, serum lipids and weight of organs of broiler chickens. British Poultry Science, vol. 44, p. 139-144. https://doi.org/10.1080/0007166031000085445, PMid:12737236

Kurtoglu, V., Kurtoglu, F., Seker, E., Coskun, B., Balevi, T., Polat, E. S. 2004. Effect of probiotic supplementation on laying hen diets on yield performance and serum and egg yolk cholesterol. Food Additives and Contaminants, vol. 21, no. 9, p. 817-823. https://doi.org/10.1080/0265203031000163 9530 PMid:15666974

Li, L., Xu, C. L., Ji, C., Ma, Q., Hao, K., Jin, Z. Y., Li, K. 2006. Effects of a dried Bacillus subtilis culture on egg quality. Poultry Science, vol. 85 no. 2, p. 364-368. PMid:16523640

Lyon, T. P. 1987. Probiotics: an alternative to antibiotics. Pig News Information, vol. 8, no. 2, p. 157-164.

Mahdavi, A. H., Rahmani, H. R., Pourreza, J. 2005. Effect of probiotic supplements on egg quality and laying hens performance. International Journal Poultry Science, vol. 4, no. 4, p. 488-492.

Mohan, B., Kadirvel, R., Bhaskaran, M., Natarajan, A. 1995. A Effect of probiotic supplementation on serum/yolk cholesterol and on egg shell thickness in layers. British Poultry Science, vol. 36, p. 799-803. https://doi.org/10.1080/00071669508417824 PMid:8746981

Mikulski, D., Jankowski, J., Naczmanski, J., Mikulska, M., Demey, V. 2012. Effects of dietary probiotic (Pediococcus acidilactici) supplementation on performance, nutrient digestibility, egg traits, egg yolk cholesterol, and fatty acid profile in laying hens. Poultry Science, vol. 91, no. 10 2691-2700. https://doi.org/10.3382/ps.2012-02370 PMid: 22991559

Nahashon, S. N., Nakaue, H. S., Mirosh, L. W. 1992. Effect of direct-fed microbials on nutrient retention and production parameters of laying pullets. Poultry Science, vol. 71 (suppl. 1), p. 111.

Nahashon, S. N., Nakaue, H. S., Mirosh, L. W. 1993. Effect of direct fed microbials on nutrient retention and productive parameters of Single Comb White Leghorn pullets. Poultry Science, vol. 72 (Suppl. 1), p. 87.

Nelson, C. R., Gilliland, S. E. 1984. Cholesterol uptake by Lactobacillus acidophilus. Journal Dairy Science, vol. 67 (Suppl. 1): p. 50.

Patterson, J. A., Burkholder, K. 2003. Application of prebiotics and probiotics in poultry production. Poultry Science, vol. 82, no. 4, p. 627-631. PMid:12710484

Prins, R. A. 1977. Biochemical activities of gut microorganisms. In Clarke, R. T. J., Hauchop, T.: Microbial ecology of the gut. Academic press : London, p. 73-183.

Ramasamy, K., Abdullah, N., Jalaludin, S., Wong, M., Ho, Y. W. 2009. Effects of Lactobacillus cultures on performance of laying hens, and total cholesterol, lipid and fatty acid composition of egg yolk. Journal of the Science of Food and Agriculture, vol. 89, 2009, no. 3, p. 482-486. https://doi.org/10.1002/jsfa.3477

Rolf, R. D. 2000. The role of probiotic cultures in the control of gastrointestinal health. Journal of Nutrition, vol. 130, p. 396S-402S. PMid:10721914

Saarela, M., Mogensen, G., Fonden, R., Matto, J., Mattila-sandholm, T. 2000. Probiotic bacteria: safety, functional and technological properties. Journal of Biotechnology, vol. 84, p. 197-215. https://doi.org/10.1016/S0168-1656(00)00375-8

SAS Institute. SAS User´s Guide: Statistics, 5th Edn.; SAS : Cary, NC, USA, 1985.

Sun, X. 2005. Broiler performance and intestinal alterations when fed drug-free diets : Thesis. Virginia Polytechnic Institute and State University, Blacksburg, Virginia.

Toms, C., Powrie, F. 2001. Control of intestinal inflammation by regulatory T cells. Microbes and Infection, vol. 3, p. 929-935. https://doi.org/10.1016/S1286-4579(01)01454-X

Yalcin, S., Guclu, B. K., Oguz, F. K. 2002. The usage of enzyme, probiotic and antibiotic in laying hen rations. Ankara Universitesi Veteriner Fakultesi Dergisi, vol. 49, 2002, no. 2., p. 135-141.

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Published

2013-07-09

How to Cite

Angelovičová, M. ., Alfaig, E. ., Král, M. ., & Tkáčová, J. . (2013). The effect of the probiotics Bacillus subtilis (PB6) on the selected indicators of the table eggs quality, fat and cholesterol. Potravinarstvo Slovak Journal of Food Sciences, 7(1), 80–84. https://doi.org/10.5219/271

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