Microscopic determination of bamboo fiber in meat products
Keywords:fiber, polarization microscopy, image analysis, vysoÄina salami
Fiber, a suitable additive to meat products with water-holding capacity, reduces curing losses and maintains juiciness of the meat. The risk is the use of excessive amounts of flour or other ingredients of vegetable origin, in which the fiber is contained. In some cases, sensory characteristics of products can be affected. Detection of fiber may be prevention of adulteration in some meat products. It is therefore very important to regularly detect the amount of fiber in meat products and check its contents. Fiber in meat products can be detected by various methods, applied are for example gravimetric, spectroscopic, histochemical, and microscopic methods. For this reason, a model meat product (Vysočina salami) was prepared in our experiment with the addition of bamboo fiber of selected concentrations of 0%, 2%, and 3%. Subsequently, a series of microscopic sections was made on different days of curing (day no. 7, 14 of the drying phase and 28, 42 of storage). Individual sections were examined and captured using a polarization microscope, the amounts of fiber in individual sections were analyzed by means of image analysis software and the values obtained were compared with each other. Also the influence of drying on the measured area of fiber in sections was monitored. The results indicate a noticeable reduction in the area of fiber until the seventh day of ripening, which is caused by the rapid loss of water in the product. In contrast, sections of products from the following days of drying contained mildly increased concentrations of fiber, which was caused by gradual drying of the products, while the area of fiber refrained form becoming smaller. Between the individual days of drying, a difference that was statistically significant was demonstrated from the 14th day of (storage or drying). Correlation was observed between the date of (storage or drying) and amount of added fiber. Among the tested mean values for the sample with the addition of fiber concentration of 2 % an insignificant difference was found. The difference between test values (day/fiber) in the sample with addition of 3% fiber was, however, statistically significant.
Brychtová, S., Hlobilková, A. 2008. Histopatologický atlas. 1st ed. Prague: Grada, 112 p. ISBN 9788024716503.
Čáslavková, P., Pospiech, M., Štarha, P., Kameník, J., Saláková, A., Tremlová, B., Řezáčová Lukášková, Z. Verwendung der Bildanalyse für die Bewertung des Anschnitts der Rohwurstsorte Poličan. (The use of digital image analysis for the evaluation of slices of the dry fermented sausage type Poličan). Fleischwirtschaft, 2014, vol. 94, no. 6, p. 88-93.
Davídek, J. 1981. Laboratorní příručka analýzy potravin (Laboratory Manual of Food Analysis). 2nd ed. Prague: SNTL, 718 p.
Decree of Ministry of Agriculture No. 326/2001 Coll., implementing § 18 letter a), d), g), h), i) and j) of Act no. 110/1997 Coll. on foodstuffs and tobacco products and amendment and supplement to some connected Acts, in the wording of later regulations, for meat, meat products, fish, other water animals and products thereof, eggs and eggs products. Collection of Acts, no. 126, p. 7414-7444.
Dhingra, D., Michael, M., Rajput, H., Patril R. T. 2012. Dietary fibre in foods: a review. J. Food Sci. Technol., vol. 49, no. 3, p. 255-266. https://doi.org/10.1007/s13197-011-0365-5 PMC:4022914
Heinz, G., Hautzinger, P. 2007. Meat processing technology: For small - to medium - scale producers [online]. Bangkok: Food and Agriculture Organization of the United Nations, 456 s. ISBN 978-974-7946-99-4.
[cit. 2015-01-12] Available at: http://www.fao.org/docrep/010/ai407e/ai407e00.htm
Kameník, J. 2012. Hygiena a technologie masa: Trvanlivé masné výrobky [online] (Hygiene and Technology of Meat: Durable Cured Meat Products). 1st ed. Brno: Veterinární a farmaceutická univerzita Brno, 117 p., ISBN 978-80-7305-608-7. Available at: http://cit.vfu.cz/ivbp/wp-content/uploads/2011/07/Kamenik-skripta-web.pdf
Litschmannová, M. 2011. Úvod do statistiky [online] (Introduction to Statistics). Ostrava: VŠB - TU Ostrava, Fakulta elektrotechniky a informatiky, 380 p. Available at: http://mi21.vsb.cz/sites/mi21.vsb.cz/files/unit/uvod_do_statistiky.pdf
Mlček, J., Rop, O., Šustová, K., Simeonovová, J., Gál, R. 2010. Možnosti využití spektroskopie NIR v masném průmyslu. (Possibilities of Using NIR Spectroscopy in the Meat Industry). Chemické listy, vol. 104, p. 855-860. [cit. 2015-01-17] Available at: http://www.chemicke-listy.cz/docs/full/2010_09_855-860.pdf
Mongeau, R. 2003. Dietary Fiber: Determination. Encyclopedia of Food Sciences and Nutrition, p. 1823-1833. https://doi.org/10.1016/B0-12-227055-X/00343-6
Nielsen, S. 2010. Food Analysis. USA, 557 p. ISBN 9781441914774.
Tremlová, B. 2000. Histological demonstration of bone fragments in meat products. Fleischwirtschaft. vol. 80, no. 7, p. 73-74.
Vorlíčková, M. 2010. Využití polarizační mikroskopie pro stanovení škrobu v surovinách a potravinových výrobcích (Using of polarizing microscopy for starch determination in raw materials and food products). Bachelor thesis. UVPS Brno. 79 p.
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