Crambe tataria sebeók seeds and plants grown in vitro and in vivo fatty acid composition comparison

Authors

  • Nadia Oleksandrivna Pushkarova Engineer, Genetic Engineering Department, Institute of Cell Biology and Genetic Engineering, 148 Academika Zabolotnoho St., 03143, Kyiv, Ukraine
  • Maria Sergiivna Kalista National Museum of Natural History of NAS of Ukraine, Department of Botany, 15 Bohdana Khmelnytskoho Str., 01601, Kyiv, Ukraine
  • Maxim Andriyovich Kharkhota D.K. Zabolotny Institute of Microbiology and Virology of the NASU, Department of antibiotics, 154 Akademik Zabolotnyi Str. D03680, Kyiv, GSP, Ukraine
  • Dzhamal Rakhmetov M.M.°Gryshko National Botanical Garden of National Academy of Sciences of Ukraine, Department of Cultivated Flora, 1 Timiriazievska Str., 01014, Kyiv, Ukraine
  • Mukola Viktorovich Kuchuk Institute of Cell Biology and Genetic Engineering, Department of Genetic Engineering, 148 Academika Zabolotnoho St., 03143, Kyiv, Ukraine

DOI:

https://doi.org/10.5219/646

Keywords:

threatened species, in vitro culture, GC/MS mass spectrophotometry, fatty acids

Abstract

Methods of in vitro conservation offer a number of advantages for endangered species preservation. Crambe tataria Sebeók biochemistry study (fatty acid (FA) composition, antioxidant activity (AOA), polyfructan and total soluble protein content) is fairly importaint and could show the potential value of this species in agriculture, Food and Chemical Industry or pharmacology including its use as a source of valuble genetic material and could lead to new promising sources of biofuel discovery. Also, comparison of in vitro and in vivo cultured plants could point out to the effect of in vitro culture methods on plants biochemical composition Fatty acid (FA) content was determined using Gas chromatography-mass spectrophotometry (GC/MS) of fatty acid ethers. Antioxidant activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method. Total soluble protein content was measured using Bradford method and polyfructan content determination was based upon ketosugars ability to color in the acidic environment with resorcinol. Plants that were grown under in vitro and in vivo conditions and seeds were used in this research. Obtained data showed that C. tataria plants had high AOA and total soluble protein content along with high total FA content along with high content of α-Linolenic acid and absence of erucic acid. Difference in biochemical composition between plants grown in aseptic and not aseptic conditions was shown.

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Published

2016-10-12

How to Cite

Pushkarova, N. O., Kalista, M. S., Kharkhota, M. A., Rakhmetov, D. ., & Kuchuk, M. V. (2016). Crambe tataria sebeók seeds and plants grown in vitro and in vivo fatty acid composition comparison. Potravinarstvo Slovak Journal of Food Sciences, 10(1), 494–498. https://doi.org/10.5219/646