Verification of animal species in ham and salami by DNA microarray and Real time PCR methods

Authors

  • Zuzana Drdolová Ing. Zuzana Drdolová, Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Golian Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Čurlej Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra
  • Lenka Maršálková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/831

Keywords:

Ham, Salami, DNA Microarray, RT-PCR, meat

Abstract

Consumer protection and detecting of adulteration is very important and has a wide societal impact in the economic sphere. Detection of animal species in meat products and the use of combining different methods is one of the means to achieve relevant product status. The aim of this study was to reveal whether or not the products label clearly meets the content declared by producer. In our study, 29 samples of meat products such as salami and ham obtained from stores and supermarkets in Slovakia were analyzed to detect the existing animal species according to the product label the use of Chipron LCD Array Analysis System, Meat 5.0. Products in which the presence of non-declared animal species has been detected were subjected to testing by the innuDETECT PCR Real-Time Kit, repeatedly. The results showed that 20 (68.96%) samples were improperly labeled. From in total 14 tested ham samples 11 (78.57%) products exhibited non-conformity with declared composition. Tested salami samples (15) revealed 9 (60%) incorrectly labelled products. The results obtained by DNA Microarray and Real Time PCR methods were identical, and both methods should be extensively promoted for the detection of animal species in the meat and meat products.

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References

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Published

2017-12-06

How to Cite

Drdolová, Z. ., Golian, J. ., Čurlej, J. ., & Maršálková, L. . (2017). Verification of animal species in ham and salami by DNA microarray and Real time PCR methods. Potravinarstvo Slovak Journal of Food Sciences, 11(1), 673–678. https://doi.org/10.5219/831

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