Effect of different storage conditions on the microbiological characteristics of insect
Keywords:edible insect, microbiota, microbiological safety, colony forming units, coliform bacteria
When introducing a novelty food, its safety needs to be monitored. One of the safety aspects of human health is microbial contamination. In this work, microbiological parameters of long-term stored edible insect material - mealworm (Tenebrio molitor), lesser mealworm (Alphitobius diaperinus), field cricket (Gryllus assimilis), and migratory locust (Locusta migratoria) were evaluated. The monitored indicators (colony forming units, enterobacteria, lactic acid bacteria, yeasts and moulds) were evaluated using common microbiological methods. All samples of stored insect were determined as safe for human consumption, except for the lesser mealworm sample from 2016, in which case the limit was exceeded. Sample of adult field cricket seems to be suitable for long-term storage, as it contained the lowest amount of microorganism. Sample of dried Gryllus assimilis from 2014 had the lowest microbial contamination. Further results suggest that, for long-term storage, the most suitable way of preparation is killing with boiling water, drying at 103 °C for 12 hours and subsequent hermetic packaging.
Adams, M., Moss, M. O. 2002. Food microbiology. Cambrige: The royal society of chemistry. ISBN‑10: 0‑85404-611-9. https://doi.org/10.1038/sj.ejcn.1600937 PMid:10745279
Agerholm-Larsen, L., Raben, A., Haulrik, N., Hansen, AS., Manders, M., Astrup, A. 2000. Effect of 8 week intake of probiotic milk products on risk factors of cardiovascular dideases. European Journal of Clinical Nutrition, vol. 54, no. 4, p. 288‑297.
ČSN 56 9609:2008. Pravidla správné hygienické a výrobní praxe - Mikrobiologická pravidla pro potraviny. Princip stanovení a aplikace. (Rules of Good Hygienic and Production Practice - Microbiological Rules for Food. Principle of determination and application.)
EFSA, 2015. Risk pro Available at: file related to production and consumption of insects as food and feed. [online], [cit.2016-02-02]. Available at: http://www.efsa.europa.eu/de/efsajournal/pub/4257
Grabowski, N. T., Nowak B., Klein G. 2008. Proximate chemical composition of long-horned and short-homed grasshoppers (Acheta domesticus, Schistocerca gregaria and Phymateus saxosus) available commercially in Germany. Archiv für Lebensmittelhygiene, vol. 59, no. 6, p. 204‑208. https://doi.org/10.1016/j.ijfoodmicro.2016.11.005 PMid:27903420
Grabowski, N. T., Klein, G. 2017. Microbiology of processed edible insect products - Results of a preliminary survey. International Journal of Food Microbiology, vol. 243, p. 103‑107.
Hanboonsog, Y., Durst, P. 2014. Edible insects in Lao PDR: building on tradition to enhance food security, Bangkok (Thailand): FAO 2014, 55 p. ISBN 978‑92‑5‑108307-9.
van Huis, A., van Itterbeeck, J., Klunder, H., Mertens, E., Halloran, A., Muir, G., Vantomme, P. 2013. Edible insects: Future prospects for food and feed security. Rome, Italy: FAO UN, Forestry Department, 201 p. Retrieved from the web: http://www.fao.org/docrep/018/i3253e/i3253e.pdf
ISO 4832:2006. Microbiology of food and animal feeding stuffs-horizontal method for the enumeration of coliforms-colony-count technique.
ISO 4833-2:2013. Microbiology of the food chain-horizontal method for the enumeration of microorganisms-part 2: colony count at 30 degrees C by the surface plating technique.
ISO 15214:1998. Microbiology of food and animal feeding stuffs-horizontal method for the enumeration of mesophilic lactic acid bacteria-colony-count technique at 30 °C.
ISO 21527-1:2008. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of yeasts and moulds - Part 1: Colony count technique in products with water activity greater than 0,95.
Klunder, H. C., Wolkers-Rooijackers, J., Korpela, J. M., Nout, M. J. R. 2012. Microbiological aspects of processing and storage of edible insects. Food Control, vol. 26, p. 628‑631. https://doi.org/10.1016/j.foodcont.2012.02.013
Regulation (EC) 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. OJ L 338/1, 22.12.2005, p. 1-26.
van der Spiegel, M., Noordam, M., van der Fels-Klerx, J. 2013. Safety of Novel Protein Sources (Insects, Microalgae, Seaweed, Duckweed, and Rapeseed) and Legislative Aspects for Their Application in Food and Feed Production. Comprehensive Reviews in Food Science and Food Safety, vol. 12, p. 662‑678. https://doi.org/10.1111/1541-4337.12032
Ssepuuya, G., Mukisa, I. M., Nakimbugwe, D. 2017. Nutritional composition, quality, and shelf stability of processed Ruspolia nitidula (edible grasshoppers). Food Science & Nutrition, vol. 5, no. 1, p. 103‑112. https://doi.org/10.1002/fsn3.369
Stoops, J., Crauwels, S., Waud, M., Claes, J. 2016. Microbial community assessment of mealworm larvae (Tenebrio molitor) and grasshoppers (Locusta migratoria migratorioides) sold for human consumtion, Food Microbiology, vol. 53, part B, p. 122‑127. https://doi.org/10.1016/j.fm.2015.09.010
Suchánková, J. 2016. Mikrobiální kvalita vybraných druhů jedlého hmyzu (Microbial quality of selected species of edible insect): diplomat theses. Zlín, Czech republic : Tomas Bata University in Zlin. 90 p.
Tančinová, D., Maková, J., Felšociová, S., Kačániová, M., Kmeť, V. 2008. Mikrobiológia potravín. (Food microbiology) Nitra: Slovak University of Agriculture in Nitra. 144 p.
Tančinová, D., Mokrý, M., Barboráková, Z., Mašková, Z. 2014. Mycobiota of spices and aromatic herbs. Potravinarstvo, vol. 8, no. 1, 2014, p. 172-177. https://doi.org/10.5219/375
Vandeweyer, D., Lievens, B., Van-Campenhout, L. 2015. Microbial quality of edible insects reared on industrial scale in Belgium and the Netherlands. Conference Paper. In nnovations in Food Packaging, Shelf Life and Food Safety. Munich, Germany, 15 - 17 September 2015, Abstract No. P1.35.
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