Occurence and antimicrobial resistance of common udder pathogens isolated from sheep milk in Slovakia

Authors

  • Ivan Holko VETSERVIS, s.r.o. Kalvária 3, 94901 Nitra, Slovakia https://orcid.org/0000-0002-8273-9241
  • Vladimí­r Tančin Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of veterinary science, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia; NPPC-Research Institute for Animal Production Nitra, Hlohovecká 2, 95141 Lužianky, Slovakia https://orcid.org/0000-0003-2908-9937
  • Kristí­na Tvarožková Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Veterinary Disciplines, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia https://orcid.org/0000-0003-4989-6138
  • Peter Supuka VETSERVIS, s.r.o., Kalvária 3, 94901 Nitra, Slovakia
  • Anna Supuková VETSERVIS, s.r.o., Kalvária 3, 94901 Nitra, Slovakia
  • Mačuhová Lucia NPPC-Research Institute for Animal Production Nitra, Hlohovecká 2, 95141 Lužianky, Slovakia https://orcid.org/0000-0002-9624-1348

DOI:

https://doi.org/10.5219/1067

Keywords:

sheep, milk, mastitis, pathogen, antibiotic

Abstract

The aim of this work is to identify the spectrum, frequency and antimicrobial resistance of bacterial pathogens occurring in sheep dairy herds in Slovakia. Of a total of 310 samples of sheep's milk coming from three breeds during two seasons (2017 and 2018), at least one potential pathogen was isolated from 102 samples (32.9%). A total of 131 microbial isolates were isolated. The most represented species were coagulase negative staphylococci CoNS (75.6%), followed by Streptococcus agalactiae (10.7%), Staphylococcus aureus (6.9%), Streptococcus dysgalactiae (4.6%), Escherichia coli (1.5%), Enterococcus faecium (1.5%), and others (Streptococcus uberis, Streptococcus parauberis, Candida sp., Klebsiella sp., moulds) below 1%. A total of 99 isolates of CoNS were tested for antimicrobial resistance. Of these, 63.6% were resistant to at least one antibiotic. A total of 24.2% of the tested isolates were resistant to 3 groups of antimicrobials simultaneously (multi-drug resistance). The highest resistance was observed to lincomycin (57.6%) and neomycin (36.4%), the lowest to sulfamethoxazolum+trimethoprim (0%) and enrofloxacin (3.0%). Based on the results of this work, it is possible to assume a similar spectrum of pathogens and their antimicrobial resistance described in the literature also within the Slovak sheep  farms focused on milk production.

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Published

2019-04-27

How to Cite

Holko, I. ., Tančin, V. ., Tvarožková, K., Supuka, P., Supuková, A., & Lucia, M. (2019). Occurence and antimicrobial resistance of common udder pathogens isolated from sheep milk in Slovakia. Potravinarstvo Slovak Journal of Food Sciences, 13(1), 258–261. https://doi.org/10.5219/1067

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