Assessment of antimicrobial potential of substances isolated from some wastes of meat processing industry
Keywords:AMP, antimicrobial activity, slaughter wastes, flow cytometry, mucous membranes
The slaughter of farm animals generates a large number of by-products. Meat waste management includes various methods, but cost-effective technologies are still in priority. This manuscript reports the results of the study of antimicrobial activity of substances isolated from such wastes of meat processing industry as bovine and pork mucous membranes and epithelial tissues. Proteomic study included two-dimensional electrophoresis with following mass spectrometric identification. Antimicrobial activity against L. monocytogenes, P. aeruginosa and S. aureus of neutralized native extracts and after enzymatic treatment as well as its ultrafiltrates was determined by flow cytometry with EvaGreen and PI dyes. It was shown that a large number of histones were found in bovine mucous membranes as well as several tissue-specific proteins, which would be a precursor of bioactive peptides. Bovine mucous membranes of the tongue and nasal cavity possessed the greatest activity in relation to P. aeruginosa, the rate of surviving cells decreased to 22.0%. Bovine mucous membranes of the rectum and the oral cavity, submandibular lymph nodes, pig mucous membranes of the larynx, tongue, lips, and rectum increased dead cells count up to 40% of all cells. Bovine nasal mucosa and pork mucous of labial cavity possessed the greatest activity against S. aureus, the rate of surviving cells did not exceed 10.0%. Determination of antimicrobial action against L. monocytogenes of native samples and treated with trypsin showed that bovine mucous membranes of the rectum and oral cavity, pork mucosa of the lips and submandibular glands were the most active. Treatment with trypsin or ultrafiltration demonstrated different effects on activity of samples. It was shown the perspectivity of recycling of such type of by-products into effective and demanded substances which can be used, for example, in the food industry as an alternative to chemical preservatives.
Abaturov, А. Е. 2011a. Cationic Antimicrobial Peptides of Non-Specific Respiratory Protection: Defensins and Cathelicidins. Defensins - Molecules Undergoing Renaissance (Part 1). Child`s health, vol. 5, no. 34, p. 161-171.
Abaturov, А. Е. 2011b. Cationic Antimicrobial Peptides of Non-Specific Respiratory Protection: Defensins and Cathelicidins. Defensins - Molecules Undergoing Renaissance (Part 2). Child`s health, vol. 5, no. 35, p. 137-144.
Adhikari, B. B., Chae, M., Bressler, D. C. 2018. Utilization of Slaughterhouse Waste in Value-Added Applications: Recent Advances in the Development of Wood Adhesives. Polymers, vol. 10, no. 2, p. 1-28. https://doi.org/10.3390/polym10020176
Alao, B. O., Falowo, A. B., Chulayo, A., Muchenje, V. 2017. The Potential of Animal By-Products in Food Systems: Production, Prospects and Challenges. Sustainability, vol. 9, no. 7, p. 1-18. https://doi.org/10.3390/su9071089
Antimicrobial Peptide Database. 2019. Available at: http://aps.unmc.edu/AP/main.php
Arvanitoyannis, I., Ladas, D. 2008. Meat waste treatment methods and potential uses. International Journal of Food Science & Technology, vol. 43, no. 3, p. 543-559. https://doi.org/10.1111/j.1365-2621.2006.01492.x
Banks, C. J., Wang, Z. 2004. Treatment of Meat Wastes. In Wang L. K. et al. Handbook of Industrial and Hazardous Wastes Treatmen. 2nd ed. Boca Raton, USA : CRC Press, Taylor & Francis Group, p. 738-775. ISBN 9780824741143. https://doi.org/10.1201/9780203026519.ch15
Barbut, S. 2015. Waste treatment and by-products. In Barbut, S. The science of poultry and meat processing. Guelph, Canada : University of Guelph, p. 743-746. ISBN 978-0-88955-626-3.
Bosch-Marcé, M., Mohan, K. V., Gelderman, M. P., Ryan, P. L., Russek-Cohen, E., Atreya, C. D. 2014. Preclinical safety evaluation of human platelets treated with antimicrobial peptides in severe combined immunodeficient mice. Transfusion, vol. 54, no. 3, p.569-576. https://doi.org/10.1111/trf.12318
Hamawand, I. Pittaway, P., Lewis, L., Chakrabarty, S., Caldwell, J., Eberhard, J., Chakraborty, A. 2017. Waste management in the meat processing industry: Conversion of paunch and DAF sludge into solid fuel. Waste Management, vol. 60, p. 340-350. https://doi.org/10.1016/j.wasman.2016.11.034
Helkar, P. B., Sahoo, A. K., Patil N. J. 2016. Review: Food Industry By-Products used as a Functional Food Ingredients. International Journal of Waste Resources, vol. 6, p. 1-6. https://doi.org/10.4172/2252-5211.1000248
Chernukha, I., Fedulova, L., Kotenkova, E., Akhremko, A. 2018. Hypolipidemic action of the meat product: in vivo study. Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 566-569. https://doi.org/10.5219/959
International UniProt Protein Database. 2019. Available at: http://www.uniprot.org/
Irshad, A., Sharma, B. D. 2015. Abattoir by-Product Utilization for Sustainable Meat Industry: A Review. Journal of Animal and Veterinary Advances, vol. 5, no. 6, p. 681-696. https://doi.org/10.5455/japa.20150626043918
Jarczak, J., Kościuczuk, E. M., Lisowski, P., Strzałkowska, N., Jóźwik, A., Horbańczuk, J., Krzyżewski, J., Zwierzchowski, L., Bagnicka, E. 2013. Defensins: Natural component of human innate immunity. Human Immunology, vol. 74, no. 9, p. 1069-1079. https://doi.org/10.1016/j.humimm.2013.05.008
Jayathilakan, K., Sultana, K., Radhakrishna, K., Bawa, A. S. 2012. Utilization of byproducts and waste materials from meat, poultry and fish processing industries: a review. Journal of Food Science and Technology, vol. 49, no. 3, p. 278-293. https://doi.org/10.1007/s13197-011-0290-7
Jędrejek, D., Levic, J., Wallace, J., Oleszek, W. 2016. Animal by-products for feed: characteristics, European regulatory framework, and potential impacts on human and animal health and the environment. Journal of Animal and Feed Sciences, vol. 25, p. 189-202. https://doi.org/10.22358/jafs/65548/2016
Kokryakov, V. N. 1995. Physico-chemical and functional properties of antimicrobial proteins and peptides : dissertation theses. Saint Petersburg, Russia : Saint Petersburg Medical Academy, 48 p.
Kotenkova, E., Bataeva, D., Minaev, M., Zaiko, E. 2019. Application of EvaGreen for the assessment of Listeria monocytogenes АТСС 13932 cell viability using flow cytometry. AIMS Microbiology, vol. 5, no.1, p. 39-47. https://doi.org/10.3934/microbiol.2019.1.39
Kotenkova, E., Lukinova, E., Kovalyov, L. 2018. Bovine mucous membranes as a source of antimicrobial compounds. Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 667-672. https://doi.org/10.5219/976
Pasupuleti, M., Schmidtchen, A., Malmsten, M. 2012. Antimicrobial peptides: key components of the innate immune system. Critical Reviews in Biotechnology, vol. 32, no. 2, p. 143-171. https://doi.org/10.3109/07388551.2011.594423
Shamova, O. V. 1995. Physico-chemical characterization and functional properties of defensins and protegrins : dissertation theses. Saint Petersburg, Russia : Saint Petersburg State University, 24 p.
Shamova, O. V. 2013. Molecular and cellular bases of biological activity realization of leukocytes antimicrobial peptides : dissertation theses. Saint Petersburg, Russia : Saint Petersburg State University 48 p.
Toldrá, F., Mora, L., Reig, M. 2016. New insights into meat by-product utilization. Meat science, vol. 120. p. 54-59. https://doi.org/10.1016/j.meatsci.2016.04.021
Virmond, E., Schacker, R. L., Albrecht, W., Althoff, C. A., Souza M., Moreira, R. F. P. M., José, H. J. 2011. Organic solid waste originating from the meat processing industry as an alternative energy source. Energy, vol. 36, p. 3897-3906. https://doi.org/10.1016/j.energy.2010.08.026
Wang, G. 2014. Human antimicrobial peptides and proteins. Pharmaceuticals (Basel), vol. 7, no. 5, p. 545-94. https://doi.org/10.3390/ph7050545
Wang, G., Li, X., Wang, Z. 2016. APD3: the antimicrobial peptide database as a tool for research and education. Nucleic Acids Research, vol. 44, p. D1087-D1093. https://doi.org/10.1093/nar/gkv1278
Wang, W, Ye, P., Qian, Y., Li, J., Sun, F., Zhang, W., Wang, X. 2015. Effects of whole cigarette smoke on human beta defensins expression and secretion by oral mucosal epithelial cells. Tobacco induced diseases, vol. 13, no. 1, p. 3. https://doi.org/10.1186/s12971-015-0029-8
Zhao, L., Lu, W. 2014. Defensins in innate immunity. Current Opinion in Hematology, vol. 21, no. 1, p.37-42. https://doi.org/10.1097/MOH.0000000000000005
Zharkova, M. S. 2016. The combined action of innate immune system proteins and peptides and compounds of different chemical nature in the implementation of their antibiotic properties : dissertation theses. Saint Petersburg, Russia : Saint Petersburg Medical Academy, p. 24.
How to Cite
LicenseAuthors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).