Influence of technological processing on lipid-lowering activity of substances containing in porcine hearts and aortas

Authors

  • Elena Kotenkova V. M. Gorbatov Federal Research Center for Food Systems of RAS, Experimental-clinical research laboratory of bioactive substances of animal origin, Talalikhina st., 26, 109316 Moscow, Russia, Tel.: +79031684478 https://orcid.org/0000-0003-1864-8115
  • Irina Chernukha V. M. Gorbatov Federal Research Center for Food Systems of RAS, Experimental-clinical research laboratory of bioactive substances of animal origin, Talalikhina st., 26, 109316 Moscow, Russia, Tel.: +79859248446 https://orcid.org/0000-0003-4298-0927

DOI:

https://doi.org/10.5219/1119

Keywords:

by-products, heart, aorta, lipid-lowering activity, functional additives and product

Abstract

Edible by-products are a good source of nutrients and bioactive substances and could be used as functional ingredients or for biopeptides production natively contained in raw materials. A wide range of peptides are also formed during the enzymatic hydrolysis or food processing. The comparative results of the effectiveness of isolated certain protein and peptide fractions by ultrafiltration with the same natively presented in raw tissues, as well as the influence of heat treatment on biological activity of origin active substances are presented. The model of rat alimentary hyperlipidemia was developed by adding cholesterol and fat to the standard diet and vitamin D2 injection per os. Serum lipid profile was determined on automatic analyzer BioChem FC-360. Dynamic of changes in serum lipid profile was assessed as corresponding control group medium results in ratio to certain rat data. Two-dimensional electrophoresis (2DE) was performed according to the method of O’Farrell with isoelectric focusing in ampholine pH gradient (IEF-PAGE) with following identification by MALDI-TOF MS and MS/MS mass spectrometry. Consumption of native pig aorta and pig heart during 14th days led to normalization of lipid profile in serum of hyperlipidemic rats, while low molecular weight (LMUF, MW <5 kDa) and medium molecular weight (MMUF, MW = 5 – 30 kDa) ultrafiltrates of pig aorta extract did not strongly influenced on level of triglicerides and, on contrary, elevated high density cholesterol. Consumption of developed product by hyperlipidemic rats during 28th days did not lead to significant changes in serum lipid profile, while on 42nd day all ratios reached ones in group, which were treated with native raw material or isolated active fractions. The stability of developed product was confirmed by proteomic studies. Obtained results open prospects to modernization the technology, presumably use as a matrix dietary meat (e.g. poultry) with incorporated active identified components.

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Author Biography

Elena Kotenkova, V. M. Gorbatov Federal Research Center for Food Systems of RAS, Experimental-clinical research laboratory of bioactive substances of animal origin, Talalikhina st., 26, 109316 Moscow, Russia, Tel.: +79031684478

1. Senior researcher of Experimental-clinical research laboratory of bioactive substances of animal origin in V.M. Gorbatov Federal Research Center for Food Systems of RAS 2. Elena A. Kotenkova has completed his PhD at the age of 25 years from V.M. Gorbatov Federal Research Center for Food Systems of RAS 3. Chernukha I.M., Fedulova L.V., Kotenkova E.A., Takeda S., Sakata R. Hypolipidemic and anti-inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model // Animal Science Journal (2018). ”“ Р. 1-10. doi:10.1111/asj.12986 Irina Chernukha, Liliya Fedulova, Elena Kotenkova. Hypolipidemic action of the meat product: in vivo study // Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, 2018, p. 566-569 L.V. Fedulova, Е.R. Vasilevskaya, Е.Ð. Kotenkova, A.A. Elkina, M.G. Baryshev, A.B. Lisitsyn Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity // Journal of Pharmacy and Nutrition Sciences. - 2017. - V. 7. - â„–2. - Р. 35-40. - DOI: https://doi.org/10.6000/1927-5951.2017.07.02.1 4. Fields of scientific interest are medicine, nutrition, agricultural and biological sciences, biochemistry, and laboratory animal science.

References

Ahhmed, A. M., Muguruma, M. 2010. A review of meat protein hydrolysates and hypertension. Meat Science, vol. 86, no. 1, p. 110-118. https://doi.org/10.1016/j.meatsci.2010.04.032

Alao, B. O., Falowo, A. B., Chulayo, A., Muchenje, V. 2017. The Potential of Animal By-Products in Food Systems: Production, Prospects and Challenges. Sustainability, vol. 9, no. 7, p.1-18. https://doi.org/10.3390/su9071089

Arrutia, F., Fernández, R., Menéndez, C., González, U. A., Riera, F. A. 2017. Utilization of blood by-products: An in silico and experimental combined study for BSA usage. Scientific Reports, vol. 7, p. 1-9. https://doi.org/10.1038/s41598-017-17029-2

Bamdad, F., Shin, S. H., Suh, J. W., Nimalaratne, C., Sunwoo, H. 2017. Anti-Inflammatory and Antioxidant Properties of Casein Hydrolysate Produced Using High Hydrostatic Pressure Combined with Proteolytic Enzymes. Molecules, vol. 22, no. 4, p. 1-16. https://doi.org/10.3390/molecules22040609

Bauchart, C., Rémond, D., Chambon, C., Patureau Mirand, P., Savary-Auzeloux, I., Reynès, C., Morzel, M. 2006. Small peptides (<5 kDa) found in ready-to-eat beef meat. Meat Science, vol. 74, no. 4, p. 658-666. https://doi.org/ https://doi.org/10.1016/j.meatsci.2006.05.016

Chernukha, I. M., Fedulova, L. V., Kotenkova, E. A. 2015. Meat by-product is a source of tissue-specific bioactive proteins and peptides against cardio-vascular diseases. Procedia Food Science, vol. 5, p. 50-53. https://doi.org/10.1016/j.profoo.2015.09.013

Chernukha, I. M., Fedulova, L. V., Kotenkova, E. A. 2018. The influence of bioactive substances isolated from porcine aorta on the serum fatty acid composition of hyperlipidemic rats. Theory and practice of meat processing, vol. 3, no. 4, p. 16-22. https://doi.org/10.21323/2414-438X-2018-3-4-16-22

Chernukha, I. M., Fedulova, L. V., Kotenkova, E. A., Shishkin, S. S., Kovalyov, L. I., Mashentseva, N. G., Klabukova, D. L. 2016 Influence of heat treatment on tissue specific proteins in the Sus Scrofa cardiac muscle and aorta. Russian Journal of Biopharmaceuticals, vol. 8, no. 6, p. 38-44.

Chernukha, I., Fedulova, L., Kotenkova, E., Akhremko, A. 2018a. Hypolipidemic action of the meat product: in vivo study. Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 566-569. https://doi.org/10.5219/959

Chernukha, I. M., Fedulova, L. V., Kotenkova, E. A., Takeda, S., Sakata, R. 2018b. Hypolipidemic and anti-inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model. Animal Science Journal, vol. 89, no 5, p. 784-793. https://doi.org/10.1111/asj.12986

Chugunov, A. O. 2010. Unknown peptides. Nauka i zhizn, no. 10, p. 26-32.

Demarin, V., Lisak, M., Morović, S., Cengić, T. 2010. Low high-density lipoprotein cholesterol as the possible risk factor for stroke. Acta Clinica Croatica, vol. 49, no. 4, p. 429-439.

Fagerberg, L., Hallström, B. M., Oksvold, P., Kampf, C., Djureinovic, D., Odeberg, J., et al. 2014. Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics. Molecular and Cellular Proteomics, vol. 13, no. 2, p. 397-406. https://doi.org/10.1074/mcp.M113.035600

Ference, B. A., Ginsberg, H. N., Graham, I., Ray, K. K., Packard, C. J., Bruckert, E., et al. 2017. Low-density lipoproteins cause atherosclerotic cardiovascular disease. 1. Evidence from genetic, epidemiologic, and clinical studies. A consensus statement from the European Atherosclerosis Society Consensus Panel. European Heart Journal, vol. 38, no. 32, p. 2459-2472. https://doi.org/10.1093/eurheartj/ehx144

Hindy, G., Engström, G., Larsson, S. C., Traylor, M., Markus, H. S., Melander, O., Orho-Melander, M. 2018. Role of Blood Lipids in the Development of Ischemic Stroke and its Subtypes: A Mendelian Randomization Study. Stroke, vol. 49, no. 4, p. 820-827. https://doi.org/10.1161/STROKEAHA.117.019653

Hongdong, S., Bo, L. 2017. Beneficial Effects of Collagen Hydrolysate: A Review on Recent Developments. Biomedical Journal of Scientific & Technical Research, vol. 1, no. 2, p. 1-4. https://doi.org/10.26717/BJSTR.2017.01.000217

Kovalyov, L. I., Kovalyova, M. A., Kovalyov, P. L., Serebryakova, M. V., Moshkovskii, S. A., Shishkin, S. S. 2006. Polymorphism of delta3,5-delta2,4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue. Biochemistry (Moscow), vol. 71, no. 4, p. 448-453. https://doi.org/10.1134/S0006297906040146

Lafarga, T., Hayes, M. 2014. Bioactive peptides from meat muscle and by-products: generation, functionality and application as functional ingredients. Meat Science, vol. 98, no. 2, p. 227-239. https://doi.org/10.1016/j.meatsci.2014.05.036

Lafarga, T., Rai, D. K., O'connor, P., Hayes, M. 2016. Generation of Bioactive Hydrolysates and Peptides from Bovine Hemoglobin with In Vitro Renin, Angiotensin‐I‐Converting Enzyme and Dipeptidyl Peptidase‐IV Inhibitory Activities. Journal of food biochemistry, vol. 40, no. 5, p. 673-685. https://doi.org/10.1111/jfbc.12259

Matthews, S. 2018. 'No evidence' having high levels of bad cholesterol causes heart disease, claim 17 physicians as they call on doctors to 'abandon' statins. Available at: https://www.dailymail.co.uk/health/article-6176151/No-evidence-having-high-levels-bad-cholesterol-causes-heart-disease.html

Mine, Y., Shahidi, F. 2006. Nutraceutical Proteins and Peptides in Health and Disease. USA : Taylor & Francis Group, CRC Press, 688 p. ISBN 9781420028836. https://doi.org/10.1201/9781420028836

Mohanty, D. P., Mohapatra, S, Misra, S, Sahu, P.S. 2016. Milk derived bioactive peptides and their impact on human health - A review. Saudi Journal of Biological Sciences, vol. 23, no. 5, p. 577-83. https://doi.org/10.1016/j.sjbs.2015.06.005

Nelson, R. H. 2013. Hyperlipidemia as a risk factor for cardiovascular disease. Primary Care, vol. 40, no. 1, p. 195-211. https://doi.org/10.1016/j.pop.2012.11.003

Toldrá, F., Aristoy, M. C., Mora, L., Reig, M. 2012. Innovations in value-addition of edible meat by-products. Meat Science, vol. 92, no. 3, p. 290-296. https://doi.org/10.1016/j.meatsci.2012.04.004

Toldrá, F., Mora, L., Reig, M. 2016.New insights into meat by-product utilization. Meat science, vol. 120. p. 54-59. https://doi.org/10.1016/j.meatsci.2016.04.021

Toldrà, M., Parés, D., Saguer, E., Carretero, C. 2011. Hemoglobin hydrolysates from porcine blood obtained through enzymatic hydrolysis assisted by high hydrostatic pressure processing. Innovative Food Science and Emerging Technologies, vol. 12, no. 4, p. 435-442. https://doi.org/10.1016/j.ifset.2011.05.002

Udenigwe, C. C., Howard, A. 2013. Meat proteome as source of functional biopeptides. Food Research International, vol. 54, no. 1, p. 1021-1032. https://doi.org/10.1016/j.foodres.2012.10.002

Wang, J., Su, Y., Jia, F., Jin, H. 2013. Characterization of casein hydrolysates derived from enzymatic hydrolysis. Chemistry Central Journal, vol. 7, no. 1. 62 p. https://doi.org/10.1186/1752-153X-7-62

Zvereva, E. A., Kovalev, L. I., Ivanov, A. V., Kovaleva, M. A., Zherdev, A. V., Shishkin, S. S., Lisitsyn, A. B., Chernukha, I. M., Dzantiev, B. B. 2015. Enzyme immunoassay and proteomic character-ization of troponin I as a marker of mammalian muscle compounds in raw meat and some meat products. Meat Science, vol. 105, p. 46-52. https://doi.org/10.1016/j.meatsci.2015.03.001

Published

2019-05-28

How to Cite

Kotenkova, E., & Chernukha, I. . (2019). Influence of technological processing on lipid-lowering activity of substances containing in porcine hearts and aortas. Potravinarstvo Slovak Journal of Food Sciences, 13(1), 331–336. https://doi.org/10.5219/1119

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