Parameters of antioxidant activity of Galega officinalis L. and Galega orientalis Lam. (Fabaceae Lindl.) plant raw material

Authors

  • Olena Vergun Gryshko National Botanical Garden of the NAS of Ukraine, Cultural Flora Department, Timiryazevska 1, 04014, Kyiv, Ukraine, Tel.: +380975398541 https://orcid.org/0000-0003-2924-1580
  • Oksana Shymanska M. M. Gryshko National Botanical Garden of the NAS of Ukraine, Cultural Flora Department, Timiryazevska 1, 04014, Kyiv, Ukraine, Tel.: +380982284804 https://orcid.org/0000-0001-8482-5883
  • Dzhamal Rakhmetov M. M. Gryshko National Botanical Garden of the NAS of Ukraine, Cultural Flora Department, Timiryazevska 1, 04014, Kyiv, Ukraine, Tel.: +380503561930
  • Olga Grygorieva M. M. Gryshko National Botanical Garden of the NAS of Ukraine, Department of Fruit Plants Acclimatisation, Timiryazevska 1, 04014, Kyiv, Ukraine, Tel.: +380671988082 https://orcid.org/0000-0003-1161-0018
  • Eva Ivanišová Slovak University of Agricultural in Nitra, Faculty of Biotechnology and Food Resources, Department of Plant Storage and Processing, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia, Tel: +421376414421
  • Jan Brindza Slovak University of Agricultural in Nitra, Faculty of Agrobiology and Food Resources, Institute of Biological Conservation and Biosafety, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia, Tel: +421376414787

DOI:

https://doi.org/10.5219/1271

Keywords:

Galega officinalis, Galega orientalis, antioxidant activity, flavonoids, phenolic acids

Abstract

The plant raw material of Galega officinalis L. (goat’s rue) and Galega orientalis Lam. (fodder galega) investigated in this study. These species are known as fodder crops with high productivity of green mass and as medicine plants. The current study was aimed to evaluate an accumulation in dry raw of selected plants the total content of phenolic acids (TPA) and flavonoids (TFC) as compounds with antioxidant activity (AA) by spectrophotometric method. AA by DPPH-method and phosphomolybdenum method (reducing power (RP)) was measured. Study of ethanolic extracts of G. officinalis showed accumulation of TPA in different organs in range from 3.65 to 15.17 mg.g-1 caffeic acid equivalent (CAE) and TFC from 10.08 to 65.75 mg.g-1 quercetin equivalent (QE), AA by DPPH-method from 6.02 to 8.45 mg.g-1 Trolox equivalent (TE) and RP of extracts by phosphomolybdenum method from 86.56 to 288.15 mg TE.g-1. In extracts of G. orientalis was identified TPA from 3.52 to 18.52 mg CAE.g-1 and TFC from 6.09 to 46.72 mg QE.g-1, antioxidant activity by DPPH-method from 6.80 to 8.48 mg TE.g-1 and antioxidant capacity by phosphomolybdenum method from 52.52 to 188.51 mg TE.g-1. It was established that less concentration of studied compounds found in the stems for both species. It should be noted that the content of phenolic acids in the leaves was decreased and flavonoids in stems increased during vegetation for both species. Content of phenolic acids in the generative organs and flavonoids in the leaves decreased in raw of G. orientalis during vegetation. Pearson’s correlation analysis demonstrated very strong relations between TFC and AA by DPPH, TPA and RP, TFC and RP for G. officinalis extracts. Very strong correlation in the extracts of G. orientalis found between TFC and RP, TPA and RP. Obtained results can be used in the further biochemical and pharmacological study.

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Published

2020-03-28

How to Cite

Vergun, O., Shymanska, O., Rakhmetov, D., Grygorieva, O., Ivanišová, E., & Brindza, J. (2020). Parameters of antioxidant activity of Galega officinalis L. and Galega orientalis Lam. (Fabaceae Lindl.) plant raw material. Potravinarstvo Slovak Journal of Food Sciences, 14, 125–134. https://doi.org/10.5219/1271

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