Effects of the administration of brewed Robusta coffee leaves on total antioxidant status in rats with high-fat, high-fructose diet-induced metabolic syndrome


  • Nurmasari Widyastuti Universitas Diponegoro, Faculty of Medicine, Department of Nutrition Science, Jl. Prof H. Soedarto, SH, Tembalang, 50275, Semarang, Indonesia, Tel.: +6281575897167, https://orcid.org/0000-0003-2964-2632
  • Gemala Anjani Universitas Diponegoro, Faculty of Medicine, Department of Nutrition Science, Jl. Prof H. Soedarto, SH, Tembalang, 50275, Semarang, Indonesia, Tel.: +6281285376785 https://orcid.org/0000-0002-7774-7693
  • Vita Gustin Almira Universitas Diponegoro, Faculty of Medicine, Department of Nutrition Science, Jl. Prof H. Soedarto, SH, Tembalang, 50275, Semarang, Indonesia, Tel.: +62896702780780 https://orcid.org/0000-0003-0432-9084
  • Suci Eka Putri Universitas Diponegoro, Faculty of Medicine, Department of Nutrition Science, Jl. Prof H. Soedarto, SH, Tembalang, 50275, Semarang, Indonesia, Tel.: +6282391182648
  • Amali Rica Pratiwi Universitas Diponegoro, Faculty of Medicine, Department of Nutrition Science, Jl. Prof H. Soedarto, SH, Tembalang, 50275, Semarang, Indonesia, Tel.: +6281232256199 https://orcid.org/0000-0003-1293-2174




metabolic syndrome, Robusta coffee leaves, total antioxidant


Robusta coffee (Coffea canephora) leaves contain phytochemical compounds and have antioxidant and anti-diabetic effects. This study investigated the effect of brewed Robusta coffee leaves on the total antioxidant status in metabolic syndrome rats. Metabolic syndrome in rats was induced by high-fat-fructose diet containing pork oil (20%), cholesterol (1.5%), cholic acid (0.5%), standard feed (80%), and fructose (1 mL per 200 g BW). The animals were categorized into normal control group (K1), metabolic syndrome control group without treatment (K2), mangiferin treatment group (X1), brewed Robusta coffee leaves 0.09 g per 200 BW group (X2), brewed Robusta coffee leaves 0.18 g per 200 BW group (X3), and brewed Robusta coffee leaves 0.36 g per 200 BW group (X4). Each dose of the coffee leaves was brewed with 3.6 mL of water at 70 °C for 10 min. The intervention was administered for 28 days. There was a significant increase in the total antioxidant status (p <0.000) in all the groups. In conclusion, the administration of brewed Robusta coffee leaves increased the total antioxidant status in metabolic syndrome rats.


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How to Cite

Widyastuti, N., Anjani, G., Almira, V. G., Putri, S. E., & Pratiwi, A. R. (2020). Effects of the administration of brewed Robusta coffee leaves on total antioxidant status in rats with high-fat, high-fructose diet-induced metabolic syndrome. Potravinarstvo Slovak Journal of Food Sciences, 14, 258–263. https://doi.org/10.5219/1350