Pollen diversity in honeys of the Czech Republic in the 2019 season

Authors

  • Matej Pospiech University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Plant Origin Foodstuffs Hygiene and Technology, Palackeho tr. 1946/1, 612 42 Brno, Czech Republic, Tel.: +42041562704
  • Simona Ljasovská University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Plant Origin Foodstuffs Hygiene and Technology, Palackeho tr. 1946/1, 612 42 Brno, Czech Republic, Tel.: +42041562704
  • Dalibor Titěra Czech University of Life Science Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Zoology and Fisheries, Kamýcká 129, 165 00 Praha 6 - Suchdol, Czech Republic, Tel.: +420607985393
  • Vojtěch Kružík University of Chemistry and Technology Prague, Faculty of Food and Biochemical Technology, Department of Food Preservation, Technická 5, 166 28 Praha 6 – Dejvice, Czech Republic, Tel.: +420220443003
  • Zdeňka Javůrková University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Plant Origin Foodstuffs Hygiene and Technology, Palackeho tr. 1946/1, 612 42 Brno, Czech Republic, Tel.: +42041562704
  • Bohuslava Tremlová University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Plant Origin Foodstuffs Hygiene and Technology, Palackeho tr. 1946/1, 612 42 Brno, Czech Republic, Tel.: +42041562700

DOI:

https://doi.org/10.5219/1504

Keywords:

melissopalynology, botany taxa, pollen, biodiversity, adulteration

Abstract

Honeybees are important pollinators. As a side product of pollination, honeybees produce honey, as a natural sweetener. The source of honey depends on the hive location. In specific conditions honeybees produce monofloral honey, but more common are polyfloral kinds of honey. In this study honey from the Czech Republic in the 2019 season was evaluated by melissopalynology analysis. The common botanical taxa in the Czech Republic were determined and season impact to pollen taxa was compared for dominant pollen taxa. The taxonomic distribution of pollen in Czech honey was stable during the year. The average number of species was 11.52 taxa per sample. The dominant pollen source in Czech honey was the Brassicaceae family. The high pollen content in honey was confirmed also in the Rosacea family (fruit tree), Ubelliferacae family and Myosotis genus. During the year the pollen taxa were equally distributed in honey. Seasonal effects were confirmed only in Salix genus, Ubelliferacae family and Phacelia genus. Seasonal effects correspond with the blooming season and honeybee handling in the hive was also confirmed. High variability during the season and hive location was confirmed for other taxa.

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References

Abou-Shaara, H. F. 2015. Potential Honey Bee Plants of Egypt. Cercetari Agronomice in Moldova, vol. 48, no. 2, p. 99-108. https://doi.org/10.1515/cerce-2015-0034

Aronne, G., de Micco, V. 2010. Traditional melissopalynology integrated by multivariate analysis and sampling methods to improve botanical and geographical characterisation of honeys. Plant Biosystems, vol. 144, no. 4, p. 833-840. https://doi.org/10.1080/11263504.2010.514125

Atanassova, J., Yurukova, L., Lazarova, M. 2012. Pollen and inorganic characteristics of bulgarian unifloral honeys. Czech Journal of Food Sciences, vol. 30, no. 6, p. 520-526. https://doi.org/10.17221/44/2012-cjfs

Avni, D., Hendriksma, H. P., Dag, A., Uni, Z., Shafir, S. 2014. Nutritional aspects of honey bee-collected pollen and constraints on colony development in the eastern Mediterranean. Journal of Insect Physiology, vol. 69, no. C, p. 65-73. https://doi.org/10.1016/j.jinsphys.2014.07.001

Bibi, S., Husain, S. Z., Naseem, M. R. 2008. Pollen analysis and heavy metals detection in honey samples from seven selected countries Association of Urinary Fluoride and Arsenic with Cognitive Development, Lipid Peroxidation and gene polymorphism in children View project Life Cycle Assessment of Pa. Article in Pakistan Journal of Botany, vol. 40, no. 2, p. 507-516.

Čeksteryte, V., Kurtinaitiene, B., Balžekas, J. 2013. Pollen diversity in honey collected from Lithuania’s protected landscape areas. Proceedings of the Estonian Academy of Sciences, vol. 62, no. 4, p. 277-282. https://doi.org/10.3176/proc.2013.4.08

Cunningham, S. A., Fournier, A., Neave, M. J., Le Feuvre, D. 2016. Improving spatial arrangement of honeybee colonies to avoid pollination shortfall and depressed fruit set (T. Diekötter, Ed.). Journal of Applied Ecology, vol. 53, no. 2, p. 350-359. https://doi.org/10.1111/1365-2664.12573

Danihelka, J. 2013. Botanické součty, rozdíly a podíly (Botanical totals, differences and shares), Živa: časopis pro biologickou práci (Živa: A Journal For Biological Work), p. 69-72. (In Czech)

Danner, N., Molitor, A. M., Schiele, S., Härtel, S., Steffan-Dewenter, I. 2016. Season and landscape composition affect pollen foraging distances and habitat use of Honey bees. Ecological Applications, vol. 26, no. 6, p. 1920-1929. https://doi.org/10.1890/15-1840.1

Demianowicz, Z. 1964. Charakteristik der einartenhonige. Annales de l’Abeille, vol. 7, no. 4, pp. 273-288. https://doi.org/10.1051/apido:19640401

Dobre, I., Alexe, P., Escuredo, O., Seijo, C. M. 2013. Palynological evaluation of selected honeys from Romania. Grana, vol. 52, no. 2, p. 113-121. https://doi.org/10.1080/00173134.2012.724443

Dötterl, S., Glück, U., Jürgens, A., Woodring, J., Aas, G. 2014. Floral Reward, Advertisement and Attractiveness to Honey Bees in Dioecious Salix caprea (A. G. Dyer, Ed.). PLoS ONE, vol. 9, no. 3, p. e93421. https://doi.org/10.1371/journal.pone.0093421

Downey, G., Hussey, K., Daniel Kelly, J., Walshe, T. F., Martin, P. G. 2005. Preliminary contribution to the characterisation of artisanal honey produced on the island of Ireland by palynological and physico-chemical data. Food Chemistry, vol. 91, no. 2, p. 347-354. https://doi.org/10.1016/j.foodchem.2004.06.020

El-Labban, M. 2020. Beekeepers’ Guide For Pollen Identification Of Honey 9789953051840 by Mohammad El-Labban - Paperback - first - 2020 - from Moh Lab (SKU. 472014).

Feás, X., Pires, J., Iglesias, A., Estevinho, M. L. 2010. Characterization of artisanal honey produced on the Northwest of Portugal by melissopalynological and physico-chemical data. Food and Chemical Toxicology, vol. 48, no. 12, p. 3462-3470. https://doi.org/10.1016/j.fct.2010.09.024

Feas, X., Vazquez-Tato, M. P., Estevinho, L., Seijas, J. A., Iglesias, A. 2012. Organic bee pollen. Botanical origin, nutritional value, bioactive compounds, antioxidant activity and microbiological quality. Molecules, vol. 17, no. 7, p. 8359-8377. https://doi.org/10.3390/molecules17078359

Da Fernandez, I. A., Ortiz, P. L. 1994. Pollen contamination of honey by bees inside the hive. Grana, vol. 33, no. 4-5, p. 282-285. https://doi.org/10.1080/00173139409429011

Gençay Çelemli, Ö., Özenirler, Ç., Ecem Bayram, N., Zare, G., Sorkun, K. 2017. Kars Balının Coğrafi İşaretlemesi İçin Melissopalinolojik Analiz. Kafkas Universitesi Veteriner Fakultesi Dergisi, vol. 24, no. 1, p. 53-59. https://doi.org/10.9775/kvfd.2017.18262

Grulich, V. 2012. Red List of vascular plants of the Czech Republic: 3rd edition. Preslia, vol. 84, no. 3, p. 631-645.

van der Ham, R. W. J. M., Kaas, J., Kerkvliet, J. 1999. Pollenanalyse, Stuifmeelonderzoek van Honing voor Imkers, Scholen en Laboratoria. 1st ed Hilvarenbeek, ND: Stichting Landelijk Proefbedrijf voor Insektenbestuiving en Bijenhouderij Ambrosiushoeve, 156 p. ISBN 9789080543812

Jerković, I., Marijanović, Z. 2010. Volatile composition screening of Salix sp. nectar honey. Benzenecarboxylic acids, norisoprenoids, terpenes, and others. Chemistry and Biodiversity, vol. 7, no. 9, p. 2309-2325. https://doi.org/10.1002/cbdv.201000021

Jerković, I., Marijanović, Z., Kranjac, M., Radonić, A. 2015. Comparison of Different Methodologies for Detailed Screening of Taraxacum officinale Honey Volatiles. Natural Product Communications, vol. 10, no. 2, p. 357-360. https://doi.org/10.1177/1934578x1501000238

Jones, G. D., Bryant, V. M. 2014. Pollen Studies of East Texas Honey. Palynology, vol. 38, no. 2, p. 242-258. https://doi.org/10.1080/01916122.2014.899276

Kale Sniderman, J. M., Matley, K. A., Haberle, S. G., Cantrill, D. J. 2018. Pollen analysis of Australian honey. PLOS one, vol. 13, no. 5, p. 1-24. https://doi.org/10.1371/journal.pone.0197545

Kaplan, Z. 2012. Flora and phytogeography of the Czech Republic. Preslia, vol. 84, no. 3, p. 505-573. https://doi.org/10.1007/978-3-319-63181-3_3

Karabagias, I. K., Maia, M., Karabagias, V. K., Gatzias, I., Badeka, A. V. 2020. Quality and origin characterisation of Portuguese, Greek, Oceanian, and Asian honey, based on poly-parametric analysis hand in hand with dimension reduction and classification techniques. European Food Research and Technology, vol. 246, no. 5, p. 987-1006. https://doi.org/10.1007/s00217-020-03461-8

Kuś, P. M., Jerković, I., Marijanović, Z., Kranjac, M., Tuberoso, C. I. G. 2018. Unlocking Phacelia tanacetifolia Benth. honey characterization through melissopalynological analysis, color determination and volatiles chemical profiling. Food Research International, vol. 106, p. 243-253. https://doi.org/10.1016/j.foodres.2017.12.065

Lieux, M. H. 1981. An analysis of mississipi (u.s.a.) honey : pollen, color and moisture. Apidologie, vol. 12, no. 2, p. 137-158. https://doi.org/10.1051/apido:19810205

Louveaux, J., Maurizio, A., Vorwohl, G. 1970. Methods of Melissopalynology. Bee World, vol. 51, no. 3, p. 125-138. https://doi.org/10.1080/0005772x.1970.11097312

Marco, G. Di, Canuti, L., Impei, S., Leonardi, D., Canini, A. 2012. Nutraceutical properties of honey and pollen produced in a natural park. Agricultural Sciences, vol. 03, no. 02, p. 187-200. https://doi.org/10.4236/as.2012.32022

Moar, N. T. 1985. Pollen analysis of New Zealand honey. New Zealand Journal of Agricultural Research, vol. 28, no. 1, p. 39-70. https://doi.org/10.1080/00288233.1985.10426997

Oddo, L. P., Piazza, M. G., Sabatini, A. G., Accorti, M. 1995. Characterization of unifloral honeys. Apidologie, vol. 26, no. 6, p. 453-465. https://doi.org/10.1051/apido:19950602

Odoux, J. F., Feuillet, D., Aupinel, P., Loublier, Y., Tasei, J. N., Mateescu, C. 2012. Territorial biodiversity and consequences on physico-chemical characteristics of pollen collected by honey bee colonies. Apidologie, vol. 43, no. 5, p. 561-575. https://doi.org/10.1007/s13592-012-0125-1

Persano Oddo, L., Piana, L., Bogdanov, S., Bentabol, A., Gotsiou, P., Kerkvliet, J., Martin, P., Morlot, M., Ortiz Valbuena, A., Ruoff, K., von der Ohe, K. 2004. Botanical species giving unifloral honey in Europe. Apidologie, vol. 35, no. Suppl. 1, p. S82-S93. https://doi.org/10.1051/apido:2004045

Persano Oddo, L., Piro, R. 2004. Main European unifloral honeys: descriptive sheets. Apidologie, vol. 35, no. Suppl. 1, p. S38-S81. https://doi.org/10.1051/apido:2004049

Pospiech, M., Javůrková, Z., Tremlová, B., Běhalová, H. 2019. Characterization of fruit trees pollen. Potravinarstvo Slovak Journal of Food Sciences, vol. 13, no. 1, p. 634-643. https://doi.org/10.5219/1096

Puusepp, L., Koff, T. 2014. Pollen analysis of honey from the Baltic region, Estonia. Grana, vol. 53, no. 1, p. 54-61. https://doi.org/10.1080/00173134.2013.850532

Pyšek, P., Danihelka, J., Sádlo, J., Chrtek, J., Chytrý, M., Jarošík, V., Kaplan, Z., Krahulec, F., Moravcová, L., Pergl, J., Štajerová, K., Tichý, L. 2012. Catalogue of alien plants of the Czech Republic (2nd edition): Checklist update, taxonomic diversity and invasion patterns. Preslia, vol. 84, no. 2, p. 155-255.

Roulston, T. H., Cane, J. H. 2000. “Pollen nutritional content and digestibility for animals.” Pollen and Pollination, Springer Vienna, p. 187-209. https://doi.org/10.1007/978-3-7091-6306-1_10

Soares, S., Amaral, J. S., Oliveira, M. B. P. P., Mafra, I. 2017. A Comprehensive Review on the Main Honey Authentication Issues: Production and Origin. Comprehensive Reviews in Food Science and Food Safety, vol. 16, no. 5, p. 1072-1100. https://doi.org/10.1111/1541-4337.12278

Sousa, M. E. B. C., Dias, L. G., Veloso, A. C. A., Estevinho, L., Peres, A. M., Machado, A. A. S. C. 2014. Practical procedure for discriminating monofloral honey with a broad pollen profile variability using an electronic tongue. Talanta, vol. 128, p. 284-292. https://doi.org/10.1016/j.talanta.2014.05.004

Sprague, R., Boyer, S., Stevenson, G. M., Wratten, S. D. 2016. Assessing pollinators’ use of floral resource subsidies in agri-environment schemes: An illustration using Phacelia tanacetifolia and honeybees. Peer J., vol. 2016, no. 11, p. e2677. https://doi.org/10.7717/peerj.2677

Stawiarz, E., Wróblewska, A. 2010. Melissopalynological analysis of multifloral honeys from the sandomierska upland area of Poland. Journal of Apicultural Science, vol. 54, no. 1, p. 65-75.

Titov, V. N., Mamonov, A. N. 2013. Role of sweetclover and phacelia in agriculture ecology improvement for droughty left-bank areas of Saratov region. Russian Agricultural Sciences, vol. 39, no. 4, p. 338-341. https://doi.org/10.3103/s1068367413040228

Von Der Ohe, W., Oddo, L. P., Piana, M. L., Morlot, M., Martin, P. 2004. Harmonized methods of melissopalynology. Apidologie, vol. 35, no. Suppl. 1, p. S18-S25. https://doi.org/10.1051/apido:2004050

Vorwohl, G. 1972. Das pollenspektrum von honigen aus den italienischen alpen. Apidologie, vol. 3, no. 4, p. 309-340. https://doi.org/10.1051/apido:19720402

Published

2020-11-28

How to Cite

Pospiech, M., Ljasovská, S., Titěra, D., Kružík, V., Javůrková, Z., & Tremlová, B. (2020). Pollen diversity in honeys of the Czech Republic in the 2019 season . Potravinarstvo Slovak Journal of Food Sciences, 14, 1115–1123. https://doi.org/10.5219/1504

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