Bone adaptation to simultaneous cadmium and diazinon toxicity in adult male rats


  • Hana Chovancová Constantine the Philosopher University, Faculty of Natural Sciences, Department of Zoology and Anthropology, 949 74 Nitra
  • Radoslav Omelka Constantine the Philosopher University, Faculty of Natural Sciences, Department of Botany and Genetics, 949 74 Nitra
  • Ivana Boboňová Constantine the Philosopher University, Faculty of Natural Sciences, Department of Zoology and Anthropology, 949 74 Nitra
  • Grzegorz Formicki Krakow Pedagogical University, Institute of Biology, Department of Zoology, Krakow 31 054
  • Róbert Toman Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Veterinary Sciences, 949 76 Nitra
  • Monika Martiniaková Constantine the Philosopher University, Faculty of Natural Sciences, Department of Zoology and Anthropology, 949 74 Nitra



bone, osteotoxicology, cadmium, diazinon, rats


Food contamination from natural or anthropogenic sources poses severe risks to health of human and animals. Bone is a metabolically active organ, which can be affected by various toxic substances, such as cadmium (Cd) and diazinon (DZN), leading to disruption in bone metabolic processes. The present study was designed to investigate the effect of simultaneous peroral administration to Cd and DZN on femoral compact bone structure in adult male rats. A total of twenty 1-month-old male Wistar rats were randomized into two experimental groups. In the first group (EG), young males were dosed with a combination of 30 mg CdCl2/L and 40 mg DZN/L in drinking water, for 90 days. Ten 1-month-old males without Cd-DZN intoxication served as a control group (CG). After 90 days of daily peroral exposure, evaluations of femoral bone
macro- and micro-structure were performed in each group. We found no significant differences in body weight, femoral weight, femoral length and cortical bone thickness between both groups (EG and CG). However, rats from the group EG displayed different microstructure in the middle part of the substantia compacta where primary vascular radial bone tissue appeared. In some cases, vascular expansion was so enormous that canals were also present near the periost. On the other hand, they occurred only near endosteal surfaces in rats from the control group. Moreover, a smaller number of primary and secondary osteons was identified in Cd-DZN-exposed rats. This fact signalizes reduced mechanical properties of their bones. Anyway, our results suggest an adaptive response of compact bone tissue to Cd-DZN-induced toxicity in adult male rats in order to prevent osteonecrosis.


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How to Cite

Chovancová, H. ., Omelka, R. ., Boboňová, I. ., Formicki, G. ., Toman, R. ., & Martiniaková, M. . (2014). Bone adaptation to simultaneous cadmium and diazinon toxicity in adult male rats. Potravinarstvo Slovak Journal of Food Sciences, 8(1), 107–113.

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