Species identification of enterococci by biochemical test and molecular-genetic methods

Authors

  • Monika Lavová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of evaluation and processing of animal products, Tr. A. Hlinku 2, 949 76 Nitra
  • Jana Bezeková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of evaluation and processing of animal products, Tr. A. Hlinku 2, 949 76 Nitra
  • Margita Čanigová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of evaluation and processing of animal products, Tr. A. Hlinku 2, 949 76 Nitra
  • Miroslav Kročko Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of evaluation and processing of animal products, Tr. A. Hlinku 2, 949 76 Nitra
  • Konrad Domig University of Natural Resources and Applied Life Sciences Vienna, Institute of Food Science, Food Microbiology and Hygiene Laboratory, Muthgasse 18, A-1190 Vienna, Austria

DOI:

https://doi.org/10.5219/364

Keywords:

enterococci, identification, EN-COCCUS test, PCR method

Abstract

The aim of this study was comparison different methods of species identification of enterococci. One hundred and fifty three suspected colonies were isolated from milk and dairy products (cheeses from cow´s, ewe´s and goat´s milk). On the bases of their growth on BEA agar, microscopic characteristic, results of Gram staining, catalase test and PYRAtest was thirty four isolates assigned to the genus Enterococcus. These isolates were identified by commercial biochemical test EN-COCCUS. 52.9% of them were included in species E. faecalis, 29.4% in E. faecium, 14.7% in E. durans and 2.9% in E. group III. This group includes 3 species: E. durans, E. hirae, E. faecalis asaccharolytic var. Then 16S rRNA sequencing nucleotide of all isolates was realized. Results of sequencing were compared with NCBI database. Only 14.7% of isolates were in 100% accordance. One from them was species E. durans and others were designated as E. faecium. For 20.6% of detected isolates was in accordance with more reference strains. Other isolates were identical with reference strain on 99%. For verification of all results species-specific PCR was used and 52.9% isolates were identified as species E. faecalis, 32.4% as E. faecium and 14.7% as E. durans. Strains belonging to the species E. faecalis were identified the most reliable by all used methods.

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References

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Published

2014-05-02

How to Cite

Lavová, M. ., Bezeková, J. ., Čanigová, M. ., Kročko, M. ., & Domig, K. . (2014). Species identification of enterococci by biochemical test and molecular-genetic methods. Potravinarstvo Slovak Journal of Food Sciences, 8(1), 124–129. https://doi.org/10.5219/364

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