Influence of garlic extract on antioxidant status of chicken
Keywords:poultry, garlic extract, antioxidant status
In 2006 the European Union banned the feeding of antibiotic growth promoters because of possible risk of drug resistance in human pathogens bacteria. This is the reason for the study of various phytogenic additives and their extracts as a natural source of biologically important compounds. Antimicrobial substances are a commonly included in chicken feed rations. They are used mainly as prevention against various diseases, and also to stimulate growth. The beneficial effects of garlic on animal organism resulting from their antimicrobial, antioxidative and antihypertensive properities. Studies focused on growth, conversion and meat quality of different types of animals indicate its positive effects. In our experiment we studied the influence of garlic extract in a dose of 0, 10 g and 15 g per 1 kg of chicken feed mixture. We focused on weight gains and antioxidant status of an organism. The experiment took 39 days. 54 seven-day-old chickens were included in the experiment. The chickens were weighed once a week, when aged 11, 17, 24, 31 and 38 days, at the same time of the day. The chickens had ad libitum access to feed ration and water. The chickens were taken blood sample at the end of the experiment when 39 days old. Their antioxidant status were measured using ABTS, FRAP and DPPH methods. Our results show that owing to higher concentration of garlic extract in feed ration the antioxidant status of observed chickens was increased. DPPH method showed an increase in antioxidant status of both experimental groups by 38% (a group with a dose of 10 g/kg of mixture) and by 46% (a group with a dose of 15 g/kg of mixture) compared to the control group. When using FRAP method, antioxidant status of both G10 and G15 groups increased by 24%, resp. 16%. No evidential differences in antioxidant activity between the experimental groups and control group were found using ABTS method. The supplement of garlic extract into a feed ration did not have any influence on weight gains of chickens.
Adibmoradi, M., B. Navidshad, J. Seifdavati and M. Royan. 2006. Effect of dietary garlic meal on histological strucure of small intestine in broiler chickens. The Journal of Poultry Science, vol. 43, p. 378-383. https://doi.org/10.2141/jpsa.43.378
Amagase, H., B. L. Petesch, H. Matsuura, S. Kasuga and Y. Itakura. 2001. Intake of garlic and its bioactive components. Journal of Nutrition, vol. 131, p. 955S-962S. ISSN: 0022-3166. PMid:11238796
Ashayerizadeh, O., Dastar, B., Shargh, M. S., Ashayerizadeh, A., Rahmatnejad, E., Hossaini, S. M. R. 2009. Use of garlic (Allium sativum), black cumin seeds (Nigella sativa l.) and wild mint (Mentha longifolia) in broiler chickens diets. Journal of Animal and Veterinary Advances, vol. 8, no. 9, p. 1860-1863 [cit. 2014-11-01]. Retrieved from the web: http://www.medwelljournals.com/fulltext/?doi=javaa.2009.1860.1863
Botsoglou, N. A., Christaki, E., Florou-Paneri, P., Giannenas, I., Papageorgiou, G., Spais, A. B. 2004. The effect of a mixture of herbal essential oils or α-tocopheryl acetate on performance parameters and oxidation of body lipid in broilers. South African Journal of Animal Science, vol. 34, p. 52-61. [cit. 2014-11-01]. Retrieved from the web: http://www.sasas.co.za/sites/sasas.co.za/files/botsoglouvol34no1_0.pdf
Focke, M., Feld, A., Lichtenthaler, H. K. 1990. Allicin, a naturally-occuring antibiotic from garlic, specifically inhibits acetyl-coa synthetase. Febs Letters, vol. 261, no. 1, p. 106-108. PMid:1968399
Horky,1 P. 2014a Effect of protein concentrate supplement on the qualitative and quantitative parameters of milk from dairy cows in organic farming. Annals of Animal Science, vol. 14, no. 2, p. 341-352. https://doi.org/10.2478/aoas-2014-0008
Horky, P. 2014b. Influence of increased dietary selenium on glutathione peroxidase activity and glutathione concentration in erythrocytes of lactating sows. Annals of Animal Science, vol. 14. ISSN: 2300-8733. In press
Horky, P., Jancikova, P., Sochor, J., Hynek, D., Chavis, G. J., Ruttkay-Nedecky, B., Cernei, N., Zitka, O., Zeman, L., Adam, V., Kizek, R. 2012a. Effect of organic and inorganic form of selenium on antioxidant status of breeding boars ejaculate revealed by electrochemistry. International Journal of Electrochemical Science, vol. 7, no. 7, p. 9643-9657. [cit. 2014-11-01]. Available at: http://connection.ebscohost.com/c/articles/82202749/effect-organic-inorganic-form-selenium-antioxidant-status-breeding-boars-ejaculate-revealed-by-electrochemistry
Horky, P., Jancikova, P., Zeman, L. 2012b. The effect of a supplement of chromium (picolinate) on the level of blood glucose, insulin activity and changes in laboratory evaluation of the ejaculate of breeding boars. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, vol. 60, p. 49-56. https://doi.org/10.11118/actaun201260010049
Horky, P., Ruttkay-Nedecky, B., Kremplova, M., Krystofova, O., Kensova, R., Hynek, D., Babula, P., Zitka, O., Zeman, L., Adam, V., Kizek R. 2013. Effect of different doses of organically bound selenium on antioxidant status and levels of metal ions in postpartum sows. International Journal of Electrochemical Science, vol. 8, p. 6162-6179. [cit. 2014-11-01]. Retrieved from the web: http://www.electrochemsci.org/papers/vol8/80506162.pdf
Horton, G. M. J., Fennell, M. J., Prasad, B. M. 1991. Effect od dietary garlic extract (Allium-sativum) on performance carcass composition and blood-chemistry changes in broiler-chickens. Canadian Journal of Animal Science, vol. 71, p. 939-942.
Hysing, S. C., Wiik, L. 2013. The role of seed infection level and fungicide seed treatments in control of net blotch in barley. European Journal of Plant Pathology, vol. 137, no. 1, p. 169-180. https://doi.org/10.1007/s10658-013-0230-7
Jancikova, P., Horky, P., Zeman, L. 2012. The effect of various copper sources on the trace elements profile in the hair, plasma and feaces and copper aktivity in the organism of horses. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, vol. 60, p. 145-151. https://doi.org/10.11118/actaun201260060145
Khan, R. U., Nikousefat, Z., Tufarelli, V., Naz, S., Javdani, M., Laudadio, V. 2012. Garlic (Allium sativum) supplementation in poultry diets: Effect on production and physiology. Worlds Poultry Science Journal, vol. 68, no. 3, p. 417-424. https://doi.org/10.1017/S0043933912000530
Mahmood, S., Mushtaq-Ul-Hassan, M., Alam, M., Ahmad, F. 2009. Comparative efficacy of Nigella sativa and Allium sativum as growth promoters in broilers. International Journal of Agriculture and Biology, vol. 11, p. 775-778. [cit. 2014-11-01]. Retrieved from the web: http://www.fspublishers.org/published_papers/95610_..pdf
Nevrkla, P., Čechová, M., Hadaš, Z. 2013. Evaluation of selected reproductive parametres in gilts and loss of piglets after repopulation. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, vol. 61, p. 1357-1364. [cit. 2014-11-01]. Retrieved from the web: http://acta.mendelu.cz/pdf/actaun201361051357.pdf
Onibi, G. E., Adebisi, O. E., Fajemisin, A. N., Adetunji, A. V. 2009. Response of broiler chickens in terms of performance and meat quality to garlic (Allium sativum) supplementation. African Journal of Agricultural Research, vol. 4, p. 511-517. [cit. 2014-11-01]. Retrieved from the web: http://www.academicjournals.org/journal/AJAR/article-abstract/9759AC338577
Peinado, M. J., Ruiz, R., Echavarri, A., Rubio, L. A. 2012. Garlic derivative propyl propane thiosulfonate is effective against broiler enteropathogens in vivo. Poultry Science, vol. 91, no. 9, p. 2148-2157. https://doi.org/10.3382/ps.2012-02280
Prasad, R., Rose, M. K., Virmani, M., Garg, S. L., Puri, J. P. 2009. Effect of garlic (Allium sativum) supplementation on haematological parameters in chicken (Gallus domesticus). Indian Journal of Animal Research, vol. 43, p. 157-162. ISSN: 0367-6722.[cit. 2014-11-01]. Retrieved from the web: http://www.indianjournals.com/ijor.aspx?target=ijor:ijar1&volume=43&issue=3&article=001
Raeesi, M., Hoseini-Aliabad, S. A., Roofchaee, A., Shahneh, Z. A., Pirali, S. 2010. Effect of periodically use of garlic (allium sativum) powder on performance and carcass characteristics in broiler chickens. World Academy of Science, Engineering and Technology, vol. 44, p. 1223-1229. [cit. 2014-11-01]. Retrieved from the web: http://waset.org/publications/3258/effect-of-periodically-use-of-garlic-allium-sativum-powder-on-performance-and-carcass-characteristics-in-broiler-chickens
Sochor, J., Ryvolova, M., Krystofova, O., Salas, P., Hubalek, J., Adam, V., Trnkova, L., Havel, L., Beklova, M., Zehnalek, J., Provaznik, I., Kizek, R. 2010a. Fully automated spectrometric protocols for determination of antioxidant activity: Advantages and disadvantages. Molecules, vol. 15, no. 12, p. 8618-8640. https://doi.org/10.3390/molecules15128618
Sochor, J., Salas, P., Zehnalek, J., Krska, B., Adam, V., Havel, L., Kizek, R. 2010b. Protokol pro fotometrické stanovení antioxidační aktivity v biologickém vzorku. (An assay for spectrometric determination of antioxidant activity of a biological extract). Listy Cukrovarnicke a Reparske, vol. 126, p. 416-417. [cit. 2014-11-01]. Available at: http://www.cukr-listy.cz/on_line/2010/PDF/416-417.pdf
Stanacev, V., Glamocic, D., Milosevic, N., Puvaca, N., Plavsa, N. 2011. Effect of garlic (Allium sativum L.) in fattening chicks nutrition. African Journal of Agricultural Research, vol. 6, p. 943-948. [cit. 2014-11-01]. Retrieved from the web: http://www.researchgate.net/publication/228339795_Effect_of_garlic_(allium_sativum_l.)_in_fattening_chicks_nutrition
Togashi, C. K., Fonseca, J. B., Soares, R., Da Costa, A. P. D., Ferreira, K. S., Detmann, E. 2008. Use of garlic and copper in the feeding of broiler chicks. Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science, vol. 37, no. 6, p. 1036-1041. https://doi.org/10.1590/S1516-35982008000600013
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