The influence of virus infections on antioxidant levels in the genetically modified plum variety "Honeysweet" (Prunus domestica L.)


  • Jiri Sochor Mendel University in Brno, Faculty of Horticulturae, Department of Viticulture and Enology, Valtická 337, 691 44 Lednice
  • Boris Krska Mendel University in Brno, Faculty of Horticulturae, Department of Fruit Growing, Valtická 337, 691 44 Lednice
  • Jaroslav Polak Crop Research Institute, Division of Crop Protection and Plant Health, Drnovská 406, Praque-Ruzyně
  • Tunde Jurikova Constantine the Philosopher University in Nitra, Faculty of Central European Studies, Institut for teacher training, Drazovska 4, 949 74 Nitra



Prunus domestica L., viral infection, antioxidants


It is well-known that polyphenolic compounds are found abundantly in fruit, but various kinds of diseases  lower these levels. This work measures total polyphenolic content, antioxidant activity and the levels of specific important antioxidants in fruits of the genetically modified (GM) plum variety HoneySweet, trees  which were previously inoculated with a range of different virus infections.  These were the Plum Pox virus (PPV), Prune Dwarf virus (PDV) and Apple Chlorotic Leaf-Spot virus (ACLSV). Uninoculated trees were used as controls. Antioxidant activity was measured using four different photometric  methods – DPPH (2,2-diphenyl-1-picrylhydrazyl), DMPD (N-dimethyl-p-phenylenediamine), ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) and FRAP (Ferric reducing antioxidant power). Total polyphenol content was measured using the Folin–Ciocalteau method. The profiles of 10 specific antioxidant constituents in the fruits of the GM plum variety HoneySweet were detected and analyzed, since these are of interest for their role in human diets and could play a role in the resistance of plants to viruses. Detection was made using HPLC with UV-VIS detection.  They were: gallic acid, p-coumaric acid, 4-aminobenzoic acid, chlorogenic acid, caffeic acid, ferulic acid, vanillin, rutin and quercetin. The compound with the highest concentration was chlorogenic acid (587 mg/100 g), and that with the lowest was p-coumaric acid (0.95 mg/100 g). Of the four methods of antioxidant activity used, in three the lowest levels of antioxidant activity were seen where the PPV virus was combined with ACLSV, and in three the highest levels were seen in the un-inoculated control without any infection. The highest values of total polyphenols were seen in the control (65.3 mg/100 g), followed by infection of PPV, then treatment PPV, PDV and ACLSV, then treatment PPV and PDV and finally the lowest levels were seen in treatment PPV and ACLSV (44.2 mg/100 g), which was also that with the lowest antioxidant activity.


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Capote, N., Perez-Panades, J., Monzo, C., Carbonell, E., Urbaneja, A., Scorza, R., Ravelonandro, M., Cambra, M. 2008. Assessment of the diversity and dynamics of plum pox virus and aphid populations in transgenic European plums under mediterranean conditions. Transgenic Research, vol. 17, no. 3, p. 367-377. PMid:17605085

Forcada, C. F. I., Gogorcena, Y., Moreno, M. A. 2014. Agronomical parameters, sugar profile and antioxidant compounds of "catherine" peach cultivar influenced by different plum rootstocks. International Journal of Molecular Sciences, vol. 15, no. 2, p. 2237-2254.

Gao, H., Cheng, N., Zhou, J., Wang, B. N., Deng, J. J., Cao, W. 2014. Antioxidant activities and phenolic compounds of date plum persimmon (diospyros lotus l.) fruits. J. Food Sci. Technol., vol. 51, no. 5, p. 950-956. PMid:24803703

Jaiswal, R., Karakose, H., Ruhmann, S., Goldner, K., Neumuller, M., Treutter, D., Kuhnert, N. 2013. Identification of phenolic compounds in plum fruits (Prunus salicina l. And prunus domestica l.) by high-performance liquid chromatography/tandem mass spectrometry and characterization of varieties by quantitative phenolic fingerprints. Journal of Agricultural and Food Chemistry, vol. 61, p. 12020-12031. PMid:24152059

Kaulmann, A., Jonville, M. C., Schneider, Y. J., Hoffmann, L., Bohn, T. 2014. Carotenoids, polyphenols and micronutrient profiles of brassica oleraceae and plum varieties and their contribution to measures of total antioxidant capacity. Food chemistry, vol. 155, p. 240-250. PMid:24594181

Los, J., Wilska-Jeszka, J., Pawlak, M. 2000. Polyphenolic compounds of plums (Prunus domestica). Polish Journal of Food and Nutrition Sciences, vol. 9, p. 35-38. ISSN: 1230-0322.

Miletic, N., Popovic, B., Mitrovic, O., Kandic, M., Leposavic, A. 2014. Phenolic compounds and antioxidant capacity of dried and candied fruits commonly consumed in serbia. Czech Journal of Food Sciences, vol. 32, no. 4, p. 360-368. [cit.2014-12-12] Available at:

Morabbi Najafabad, A., Jamei, R. 2014. Free radical scavenging capacity and antioxidant activity of methanolic and ethanolic extracts of plum (prunus domestica l.) in both fresh and dried samples. Avicenna journal of phytomedicine, vol. 4, p. 343-53. ISSN: 2228-7930.

Pohanka, M., Sochor, J., Ruttkay-Nedecky, B., Cernei, N., Adam, V., Hubalek, J., Stiborova, M., Eckschlager, T., Kizek, R. 2012. Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry. Journal of Applied Biomedicine, vol. 10, no. 3, p. 155-167.

Polak, J., Kumar, J., Krska, B., Ravelonandro, M. 2012. Biotech/gm crops in horticulture: Plum cv. Honeysweet resistant to plum pox virus. Plant Protection Science, vol. 48, p. S43-S48. ISSN: 1212-2580. [cit.2014-12-12] Available at:

Popov, S. V., Ovodova, R. G., Golovchenko, V. V., Khramova, D. S., Markov, P. A., Smirnov, V. V., Shashkov, A. S., Ovodov, Y. S. 2014. Pectic polysaccharides of the fresh plum Prunus domestica l. Isolated with a simulated gastric fluid and their anti-inflammatory and antioxidant activities. Food chemistry, vol. 143, p. 106-113.

Ravelonandro, M., Scorza, R., Polak, J., Callahan, A., Krska, B., Kundu, J., Briard, P. 2013. "Honeysweet" plum–a valuable genetically engineered fruit-tree cultivar. Food and Nutrition Sciences, vol. 4, p. 45-49.

Rop, O., Jurikova, T., Mlcek, J., Kramarova, D., Sengee, Z. 2009. Antioxidant activity and selected nutritional values of plums (prunus domestica l.) typical of the white carpathian mountains. Scientia Horticulturae, vol. 122, no. 4, p. 545-549.

Sanchez-Rangel, J. C., Benavides, J., Heredia, J. B., Cisneros-Zevallos, L., Jacobo-Velazquez, D. A. 2013. The folin-ciocalteu assay revisited: Improvement of its specificity for total phenolic content determination. Analytical Methods, vol. 5, p. 5990-5999.

Sochor, J., Majzlik, P., Salas, P., Adam, V., Trnkova, L., Hubalek, J., Kizek, R. 2010a. Studium dostupnosti iontů těžkých kovů pomocí různých extrakčních postupů a elektrochemické detekce (A study of availability of heavy metal ions by using various exracction procedures and electrochemical detection.) Listy Cukrovarnicke a Reparske, vol. 126, p. 414-415. ISSN: 1210-3306. [cit.2014-12-12] Available at:

Sochor, J., Ryvolova, M., Krystofova, O., Salas, P., Hubalek, J., Adam, V., Trnkova, L., Havel, L., Beklova, M., Zehnalek, J., Provaznik, I., Kizek, R. 2010b. Fully automated spectrometric protocols for determination of antioxidant activity: Advantages and disadvantages. Molecules, vol. 15, no. 12, p. 8618-8640. PMid:21116230

Sochor, J., Skutkova, H., Babula, P., Zitka, O., Cernei, N., Rop, O., Krska, B., Adam, V., Provaznik, I., Kizek, R. 2011. Mathematical evaluation of the amino acid and polyphenol content and antioxidant activities of fruits from different apricot cultivars. Molecules, vol. 16, no. 9, p. 7428-7457.

Srivastava, M., Hegde, M., Chiruvella, K. K., Koroth, J., Bhattacharya, S., Choudhary, B., Raghavan, S. C. 2014. Sapodilla plum (Achras sapota) induces apoptosis in cancer cell lines and inhibits tumor progression in mice. Scientific Reports, vol. 4.

Treutter, D., Wang, D., Farag, M. A., Baires, G. D., Ruhmann, S., Neumuller, M. 2012. Diversity of phenolic profiles in the fruit skin of prunus domestica plums and related species. J. Agric. Food Chem., vol. 60, p. 12011-12019. PMid:23140499

Utsunomiya, H., Yamakawa, T., Kamei, J., Kadonosono, K.,Tanaka, S. I. 2005. Anti-hyperglycemic effects of plum in a rat model of obesity and type 2 diabetes, wistar fatty rat. Biomedical Research-Tokyo, vol. 26, no. 5, p. 193-200. PMid:16295695

Venter, A., Joubert, E., De Beer, D. 2014. Nutraceutical value of yellow- and red-fleshed South african plums (Prunus salicina Lindl.): Evaluation of total antioxidant capacity and phenolic composition. Molecules, vol. 19, no. 3, p. 3084-3109. PMid:24619353

Vizzotto, M., Porter, W., Byrne, D., Cisneros-Zevallos, L. 2014. Polyphenols of selected peach and plum genotypes reduce cell viability and inhibit proliferation of breast cancer cells while not affecting normal cells. Food chemistry, vol. 164, p. 363-370. PMid:24996346




How to Cite

Sochor, J. ., Krska, B. ., Polak, J. ., & Jurikova, T. . (2015). The influence of virus infections on antioxidant levels in the genetically modified plum variety "Honeysweet" (Prunus domestica L.). Potravinarstvo Slovak Journal of Food Sciences, 9(1), 195–200.

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