Application of lavender and rosemary essential oils improvement of the microbiological quality of chicken quarters
Keywords:chickens quarters, microorganisms, lavender and rosemary essential oils, vacuum, EDTA
The aim of the present work was monitoring of chicken quarters microbiological indicators after treatment by ethylenediaminetetraacetate (EDTA), lavender (Lavandula angustifolia L.) and rosemary (Rosmarinus officinalis L.) essential oil, stored under vacuum packaging, at 4 ±0.5°C for a period of 16 days. The following treatments of chicken quarters were used: Air-packaging control samples, control vacuum-packaging samples, vacuum-packaging with EDTA solution 1.50% w/w, control samples, vacuum-packaging with Lavandula angustifolia essential oil at concentrations 0.2% v/w and vacuum-packaging with Rosmarinus officinalis essential oil at concentration 0.2% v/w. The quality assessment of all samples was established by microbiological analysis. Sampling was carried out after certain time intervals: 0, 4, 8, 12 and 16 days. Chicken quarters were stored under vacuum packaging, at 4 ±0.5°C during experiment. Microbiological analyses were conducted by using standard microbiological methods. Anaerobic plate count were determined using Plate Count Agar, after incubation for 2 days at 35°C under anaerobic condition. Pseudomonas spp. were determined on Pseudomonas Isolation agar after incubation at 48 h at 25°C. For lactic acid bacteria were inoculated into Rogosa and Sharpe agar after incubation 48-78 h at 37°C in an aerobic atmosphere supplemented with carbon dioxide (5% CO2). For members of the family Enterobacteriaceae violet red bile glucose agar were used and samples were incubated at 37°C for 24 h. The initial APC value of chicken quarter was 3.00 log CFU.g-1 on 0 day. The number of anaerobic plate count ranged from 3.00 log CFU.g-1 in all tested group on 0 day to 6.11 log CFU.g-1 on 16 day in control group stored in air condition. The initial LAC value of chicken quarter was 3.00 log CFU.g-1 on 0 day. The number of lactic acid bacteria ranged from 3.00 log CFU.g-1 in all tested group on 0 day to 3.58 log CFU.g-1 on 16 day in control group stored in air condition. The initial Enterobacteriacea genera value of chicken quarter was 2.00 log CFU.g-1 on 0 day. Presences of these bacteria were found on all groups at 16 days. The results of this present study suggest the possibility of application the Lavandula angustifolia and Rosmarinus officinalis essential oil as natural food preservatives and potential sources of antimicrobial ingredients for food industry.
Amaral, G. P., Carvalho, N. R., Barcelos, R. P., Dobrachinski, F., Portella, R. D. L., Silva, M. H., Lugokenski, T. H., Dias, G. R. M., da Luz, S. C. A., Boligon, A. A., Athayde, M. L., Villetti, M. A., Soares, F. A. A., Fachinetto, R. 2013. Protective action of ethanolic extract of Rosmarinus officinalis L. in gastric ulcer prevention induced by ethanol in rats. Food Chem Toxicol., vol. 55, p. 48-55. https://doi.org/10.1016/j.fct.2012.12.038 PMid:23279841
Arranz, E., Mes, J., Wichers, H. J., Jaime, L., Mendiola, J. A., Reglero, G., Santoyo, S. 2015. Anti-inflammatory activity of the basolateral fraction of Caco-2 cells exposed to a rosemary supercritical extract. Journal of Functional Foods, vol. 13, p. 384-390. https://doi.org/10.1016/j.jff.2015.01.015
Aureli, P., Costantini, A., Zolea, S. 1992. Antimicrobial activity of some plant essential oils against Listeria monocytogenes. Journal of Food Protection, vol. 55, no. 5, p. 344-348.
Babovic, N., Djilas, S., Jadranin, M., Vajs, V., Ivanovic, J., Petrovic, S., Zizovic, I. 2010. Supercritical carbon dioxide extraction of antioxidant fractions from selected Lamiaceae herbs and their antioxidant capacity. Innovative Food Science & Emerging Technologies, vol. 11, no. 1, p. 98-107. https://doi.org/10.1016/j.ifset.2009.08.013
Barreto, H. M., Silva Filho, E. C., Lima, E. D. O., Coutinho, H. D. M., MoraisBraga, M. F. B., Tavares, C. C. A., Tintino, S. R., Rego, J. V., de Abreau, A. P. L., Lustosa, M. C. G., Oliveira, R. W. G., Citó, A. M. G. L., Lopes, J. A. D. 2014. Chemical composition and possible use as adjuvant of the antibiotic therapy of the essential oil of Rosmarinus officinalis L. Industrial Crops and Products, vol. 59, p. 290-294. https://doi.org/10.1016/j.indcrop.2014.05.026
Begum, A., Sandhya, S., Ali, S. S., Vinod, K. R., Reddy, S., & Banji, D. 2013. An indepth review on the medicinal flora Rosmarinus officinalis (Lamiaceae). Acta Scientiarum Polonorum Technologia Alimentaria, vol. 12, no. 1, p. 61-73.
Bourre, J. M. 2005. Where to find omega-3-fatty acids and how feeding animals with diet enriched in omega-3-fatty acids to increase nutritional value derived products for human: what is actually useful? Journal of Nut. Health Aging, vol. 9, p. 232-242. PMid:15980924
Burt, S. 2004. Essential oils: their antibacterial properties and potential applications in foods - a review. Int J. Food Microbiol., vol. 94, p. 223-253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
Danh, L. T., Triet, N. D. A., Han, L. T. N., Zhao, J., Mammucari, R., Foster, N. 2012. Antioxidant activity, yield and chemical composition of lavender essential oil extracted by supercritical CO2. Journal of Supercritical Fluids, vol. 70, p. 27-34. https://doi.org/10.1016/j.supflu.2012.06.008
Doulgeraki, A., Ercolini, D., Villani, F., Nychas, G. E. 2012. Spoilage microbiota associated to the storage of raw meat in different conditions. International Journal of Food Microbiology, vol. 157, p. 130-141. https://doi.org/10.1016/j.ijfoodmicro.2012.05.020
Fratianni, F., Martino, L. D., Melone, A., Feo, V. D., Coppola, R., Nazzaro, F. 2010. Preservation of chicken breast meat treated with thyme and balm essential oils. Journal of Food Science, vol. 75, no. 8, p. 528-535. https://doi.org/10.1111/j.1750-3841.2010.01791.x
Gende, L. B., Maggi, M., van Baren, C., di Leo Lira, A., Bandoni, A., Fritz, R., Eguaras, M. 2010. Antimicrobial and miticide activities of Eucalyptus globulus essential oils obtained from different Argentine regions. Spanish Journal of Agricultural Research, vol. 8, no. 3, p. 642-650.
Gómez-Estaca, J., López de Lacey, A., López-Caballero, M. E., Gómez-Guillén, M. C., Montero, P. 2010. Biodegradable gelatine-chitosan films incorporated with essential oils as antimicrobial agents for fish preservation. Food Microbiology, vol. 27, p. 889-896. https://doi.org/10.1016/j.fm.2010.05.012
Goulas, A. E., Kontominas, M. G. 2007. Combined effect of light salting, modified atmosphere packaging and oregano essential oil on the shelf-life of sea bream (Sparus aurata): biochemical and sensory attributes. Food Chemistry, vol. 100, no. 1, p. 287-296. https://doi.org/10.1016/j.foodchem.2005.09.045
Govaris, A., Solomakos, N., Pexara, A., Chatzopoulou, P. S. 2010. The antimicrobial effect of oregano essential oil, nisin and their combination against Salmonella Enteritidis in minced sheep meat during refrigerated storage. Int. J. Food Microbiol., vol. 137, p. 175-180. https://doi.org/10.1016/j.ijfoodmicro.2009.12.017
Gram, L., Ravn, L., Rasch, M., Bruhn, J. B., Christensen, A. B., Givskov, M. 2002. Food spoilage interactions between food spoilage bacteria. Int. J. Food Microbiol., vol. 78, no. 1-2, p. 79-97. https://doi.org/10.1016/s0168-1605(02)00233-7 PMid:12222639
Gutierrez, J., Barry-Ryan, C., Bourke, P. 2009. Antimicrobial activity of plant essential oils using food model media: efficacy, synergistic potential and interaction with food components. Food Microbiology, vol. 26, p. 142-150. https://doi.org/10.1016/j.fm.2008.10.008
Hussain, A. I., Anwar, F., Chatha, S. A. S., Jabbar, A., Mahboob, S., Nigam, P. S. 2010. Essential oil: antiproliferative, antioxidant and antibacterial activities. Brazilian Journal of Microbiology, vol. 41, p. 1070-1107. https://doi.org/10.1590/s1517-83822010000400027
Ivanišová, E., Frančáková, H., Ritschlová, P., Dráb, Š., Solgajová, M., Tokár, M. 2015a. Biological activity of apple juice enriched by herbal extracts. Journal of Microbiology, Biotechnology and Food Science, vol. 4, Special issue 3, p. 69-73. https://doi.org/10.1590/s1517-83822010000400027
Ivanišová, E., Frančáková, H., Dráb, Š., Benčová, S. 2015b. Elderberry as important source of antioxidant and biologically active compounds. Proceedings International Scientific and Professional Conference 15th Ružiška Days, Today Science, Tomorrow Industry, Vukovar, Croatia. p. 212 221. ISBN 978-953-7005-36-8.
Ivanišová, E., Tokár, M., Mocko, K., Mendelová, A., Bojňanská, T., Mareček, J. 2013. Antioxidant activity of selected plant products. Journal of Microbiology, Biotechnology and Food Sience, vol. 2, Special issue 1, p. 1692-1703.
Jalali-Heravi, M., Moazeni-Pourasil, R. S., Sereshti, H. 2015. Elimination of chromatographic and mass spectrometric problems in GC-MS analysis of Lavender essential oil by multivariate curve resolution techniques: Improving the peak purity assessment by variable size moving window-evolving factor analysis. Journal of Chromatography B, vol. 983-984, p. 83-89. https://doi.org/10.1016/j.jchromb.2015.01.005
Jayasena, D. D., Jo, C. 2013. Essential oils as potential antimicrobial agents in meat and meat products: A review. Trends in Food Science & Technology, vol. 34, no. 2, p. 96-108. https://doi.org/10.1016/j.tifs.2013.09.002
Kačániová, M., Vukovič, N., Hleba, L., Bobková, A., Pavelková, A., Rovná, K., Arpásová, H. 2012. Antimicrobial and antiradicals activity of OriganumVulgare L. and Thymus Vulgaris essential oils. Journal of Microbiology, Biotechnology and Food Science, vol. 2, no. 1, p. 263-271.
Karabagias, I., Badeka, A., Kontominas, M. G. 2011. Shelf life extension of lamb meat using thyme or oregano essential oils and modified atmosphere packaging. Meat Science, vol. 88, p. 109-116. https://doi.org/10.1016/j.meatsci.2010.12.010
Kim, N. S., Lee, D. S. 2002. Comparison of different extraction methods for the analysis of fragrances from Lavandula species by gas chromatography-mass spectrometry. Journal of Chromatography A, vol. 982, no. 1, p. 31-47. https://doi.org/10.1016/s0021-9673(02)01445-0
Lucera, A., Costa, C., Conte, A., Del Nobile, M. A. 2012. Food applications of natural antimicrobial compounds. Frontiers in Microbiology, vol. 3, no. 287, p. 1-13. https://doi.org/10.3389/fmicb.2012.00287
Gulluce, M., Sahin, F., Sokmen, M., Ozer, H., Daferera, D., Sokmen, A., Polissiou, M., Adiguzel, A., Ozkan. H. 2007. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food Chemistry, vol. 103, p. 1449. https://doi.org/10.1016/j.foodchem.2006.10.061
Liptaiová, D., Angelovičová, M., Močár, K., Štofan, D. 2010. The effect of graduated amount of Cinnamomi aetheroleum on broiler´s production. Potravinarstvo, vol. 4, Special issue, p. 172-178. Available at: http://www.potravinarstvo.com/dokumenty/mc_februar_2010/pdf/2/Liptaiova.pdf
Marino, M., Bersani, C., Comi, G. 2001. Impedance measurements to study the antimicrobial activity of essential oils from Lamiacea and Compositae. Int. J. Food Microbiol., vol. 67, p. 187-195. https://doi.org/10.1016/s0168-1605(01)00447-0
Martucci, J. F., Gende, L. B., Neiraa, L. M., Ruseckaitea, R. A. 2015. Oregano and lavender essential oils as antioxidant and antimicrobial additives of biogenic gelatin films. Industrial Crops and Products, vol. 71, p. 205-213. https://doi.org/10.1016/j.indcrop.2015.03.079
Min, B. J., Oh, J.-H. 2009. Antimicrobial activity of catfish gelatin coating containing origanum (Thymus capitatus) oil against gram-negative pathogenic bacteria. Journal of Food Science, vol. 74, no. 4, M143-M148. https://doi.org/10.1111/j.1750-3841.2009.01115.x
Neilsen, P. V., Rios, R. 2000. Inhibition of fungal growth on bread by volatile components from spices and herbs, and the possible application in active packaging, with special emphasis on mustard essential oil. Int. J. Food Microbiol., vol. 60, p. 219-229. https://doi.org/10.1016/s0168-1605(00)00343-3
Peter, K. V. 2004. Handbook of herbs and spices. Introduction to herbs and species. K. V. Peter (Ed.), ISBN: 978-1-85573-721-1 https://doi.org/10.1533/9781855738355
Petrou, S., Tsiraki, M., Giatrakou, V., Savvaidis, I. N. 2012. Chitosan dipping or oregano oil treatments, singly or combined on modified atmosphere packaged chicken breast meat. Int. J. Food Microbiol., vol. 156, no. 3, p. 264-271. https://doi.org/10.1016/j.ijfoodmicro.2012.04.002
Radha Krishnan, K., Babuskin, S., Azhagu Saravana Babu, P., Sasikala, M., Sabina, K., Archana, G., Sivarajan, M., Sukumar, M. 2014. Antimicrobial and antioxidant effects of spice extracts on the shelf life extension of raw chicken meat. Int. J. Food Microbiol., vol. 171, p. 32-40. https://doi.org/10.1016/j.ijfoodmicro.2013.11.011
Ribeiro-Santos, R., Carvalho-Costa, D., Cavaleiro, C., Costa H. S., Albuquerque, T. G., Castilho, M. C., Ramos, F., Melo, N. R., Sanches-Silva, A. 2015. A novel insight on an ancient aromatic plant: The rosemary (Rosmarinus officinalis L.). Trends in Food Science & Technology, vol. 45, p. 355-368. https://doi.org/10.1016/j.tifs.2015.07.015
Sadaka, F., Nguimjeu, C., Brachais, C. H., Vroman, I., Tighzert, L., Couvercelle, J. P. 2013. Review on antimicrobial packaging containing essential oils and their active biomolecules. Innovative Food Science and Emerging Technologies, vol. 20, p. 350-355. https://doi.org/10.1016/j.ifset.2013.01.004
Sasikumar, B. 2012. Rosemary. In K. V. Peter (Ed.), Handbook of herbs and spices, p. 452-468. https://doi.org/10.1533/9780857095671.452
Sebranek, J. G., Sewalt, V. J. H., Robbins, K. L., Houser, T. A. 2005. Comparison of a natural rosemary extract and BHA/BHT for relative antioxidant effectiveness in pork sausage. Meat Science, vol. 69, no. 2, p. 289-296. https://doi.org/10.1016/j.meatsci.2004.07.010 PMid:22062821
Shan, B., Cai, Y. Z., Brooks, J. D., Corke, H. 2007. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. Int. J. Food Microbiol., vol. 117, no. 1, p. 112-119. https://doi.org/10.1016/j.ijfoodmicro.2007.03.003
Shobana, S., Naidu, K. A. 2000. Antioxidant activity of selected Indian spices. Prostaglandins, Leukotriens and Essential Fatty Acids, vol. 62, p. 107-110. https://doi.org/10.1054/plef.1999.0128
Skandamis, P., Tsigarida, E., Nychas, G. J. E. 2002. The effect of oregano essential oil on survival/death of Salmonella typhimurium in meat stored at 5°C under aerobic, VP/MAP conditions. Food Microbiology, vol. 19, p. 97-103. https://doi.org/10.1006/fmic.2001.0447
Szumny, A., Figiel, A., Gutierrez-Ortíz, A., Carbonell-Barrachina, A. A. 2010. Composition of rosemary essential oil (Rosmarinus officinalis) as affected by drying method. Journal of Food Engineering, vol. 97, no. 2, p. 253-260. https://doi.org/10.1016/j.jfoodeng.2009.10.019
Teixeira, B., Marques, A., Ramos, C., Neng, N. R., Nogueira, J. M. F., Saraiva, J. A., Nunes, M. L. 2013a. Chemical composition and antibacterial and antioxidant properties of commercial essential oils. Industrial Crops Production, vol. 43, p. 587-595. https://doi.org/10.1016/j.indcrop.2012.07.069
Teixeira, B., Marques, A., Ramos, C., Serrano, C., Matos, O., Neng, N. R., Nogueira, J. M., Saraiva, J. A., Nunes, M. L. 2013b. Chemical composition and bioactivity of different oregano (Origanum vulgare) extracts and essential oil. Journal of the Science of Food and Agriculture, vol. 93,
no. 11, p. 2707-2714. https://doi.org/10.1002/jsfa.6089
Viuda-Martos, M., Ruiz-Navajas, Y., Fernandez-Lopez, J., Perez-Alvarez, J. A. 2010. Effect of added citrus fibre and spice essential oils on quality characteristics and shelf-life of mortadella. Meat Science, vol. 85, p. 568-576. https://doi.org/10.1016/j.meatsci.2010.03.007
Zeleňáková, L., Kulichová, K., Kopčeková, J. 2010. Influence of soft meat products storage on their microbiological safety. Potravinarstvo, vol. 4, no. 3., p. 69-73. https://doi.org/10.5219/20
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