Histological analysis of femoral bones in rabbits administered by amygdalin


  • Veronika Kováčová Constantine the Philosopher University, Faculty of Natural Sciences, Department of Zoology and Anthropology, 949 74 Nitra
  • Radoslav Omelka Constantine the Philosopher University, Faculty of Natural Sciences, Department of Botany and Genetics, 949 74 Nitra
  • Anna Šarocká Constantine the Philosopher University, Faculty of Natural Sciences, Department of Zoology and Anthropology, 949 74 Nitra
  • Patrik Šranko Constantine the Philosopher University, Faculty of Natural Sciences, Department of Botany and Genetics, 949 74 Nitra
  • Mária Adamkovičová Constantine the Philosopher University, Faculty of Natural Sciences, Department of Botany and Genetics, 949 74 Nitra
  • Róbert Toman Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Veterinary Sciences, 949 76 Nitra
  • Marek Halenár Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, 949 76 Nitra
  • Adriana Kolesárová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, 949 76 Nitra
  • Monika Martiniaková Constantine the Philosopher University, Faculty of Natural Sciences, Department of Zoology and Anthropology, 949 74 Nitra




amygdalin, femoral bone, rabbit, histomorphometry


Cyanogenic glycosides are present in several economically important plant foods. Amygdalin, one of the most common cyanoglucoside, can be found abundantly in the seeds of apples, bitter almonds, apricots, peaches, various beans, cereals, cassava and sorghum. Amygdalin has been used for the treatment of cancer, it shows killing effects on cancer cells by release of cyanide. However, its effect on bone structure has not been investigated to date. Therefore, the objective of this study was to determine a possible effect of amygdalin application on femoral bone microstructure in adult rabbits. Four month old rabbits were randomly divided into two groups of three animals each. Rabbits from E group received amygdalin intramuscularly at a dose 0.6 mg.kg-1 body weight (bw) (group E, n = 3) one time per day during 28 days. The second group of rabbits without amygdalin supplementation served as a control (group C, n = 3). After 28 days, histological structure of femoral bones in both groups of rabbits was analysed and compared. Rabbits from E group displayed different microstructure in middle part of the compact bone and near endosteal bone surface. For endosteal border, an absence of the primary vascular longitudinal bone tissue was typical. This part of the bone was formed by irregular Haversian and/or by dense Haversian bone tissues. In the middle part of substantia compacta, primary vascular longitudinal bone tissue was observed. Cortical bone thickness did not change between rabbits from E and C groups. However, rabbits from E group had a significantly lower values of primary osteons' vascular canals and secondary osteons as compared to the C group. On the other hand, all measured parameters of Haversian canals did not differ between rabbits from both groups. Our results demonstrate that intramuscular application of amygdalin at the dose used in our study affects femoral bone microstructure in rabbits.


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How to Cite

Kováčová, V. ., Omelka, R. ., Šarocká, A. ., Šranko, P. ., Adamkovičová, M. ., Toman, R. ., Halenár, M. ., Kolesárová, A. ., & Martiniaková, M. . (2016). Histological analysis of femoral bones in rabbits administered by amygdalin. Potravinarstvo Slovak Journal of Food Sciences, 10(1), 393–399. https://doi.org/10.5219/625

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