Genetic variation and relationships of old maize genotypes (Zea mays L.) detected using SDS-page

Authors

  • Martin Vivodí­k Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Biochemistry and Biotechnology, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia,
  • Zdenka Gálová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Biochemistry and Biotechnology, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
  • Želmí­ra Balážová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Biochemistry and Biotechnology, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
  • Lenka Petrovičová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Biochemistry and Biotechnology, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
  • Tí­mea Kuťka Hlozáková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Biochemistry and Biotechnology, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia

DOI:

https://doi.org/10.5219/661

Keywords:

maize, dendrogram, SDS-PAGE, genetic diversity

Abstract

The assessment of genetic diversity among the members of a species is of vital importance for successful breeding and adaptability. In the present study 40 old genotypes of maize from Hungary, Union of Soviet Socialist Republics, Poland, Czechoslovakia, Yugoslavia and Slovak Republic  were evaluated for the total seed storage proteins using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) through vertical slab unit. The number of total scorable protein bands was twentythree as a result of SDS-PAGE technique but those that were not cosistent in reproducibility and showed occasional variation in sharpness and density were not considered. Out of twentythree polypeptide bands, 6 (31%) were commonly present in all accessions and considered as monomorphic, while 17 (65%) showed variations and considered as polymorphic. On the basis of banding profiles of proteins of different kDa, gel was divided into zones A, B and C. The major protein bands were lied in zones A and B, while minor bands were present in zones C. In zone A out of 10 protein bands, 1 were monomorphic and 9 were polymorphic. In zone B out of 8 protein bands, 3 was monomorphic and 5 was polymorphic and in zone C out of 5 protein bands, 2 were monomorphic whereas 3 polymorphic. The dendrogram tree demonstrated the relationship among the forty registered old maize genotypes according to the similarity index, using UPGMA cluster analysis. The dendrogram was divided into two main clusters. The first one contained eleven genotypes from maize, while the second cluster contained the twentynine genotypes of maize. Similarly the present study of genetic variability in the seed storage polypeptide determined by SDS-PAGE technique proved that it is fruitful to identify genetic diversity among accessions of maize.

 

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References

AL-Huqail, A. A., Abdelhaliem, E. 2015. Evaluation of Genetic Variations in maize Seedlings Exposed to Electric Field Based on Protein and DNA Markers. BioMed Research International, vol. 2015, p. 1-15. https://doi.org/10.1155/2015/874906

Bremer, K., Bremer, B., Thulin, M. 2003. Introduction of Phylogeny and Systematic of Flowering Plants. Symbolae Botanicae Upsalienses, vol.33, no. 2, p. 64-73.

Dowswell, C. R., Paliwal, R. L., Cantrell, R. P. 1996. Maize in the Third World. University of Michigan : Westview Press, ISBN: 978-0813389639.

Dymek, K., Dejmek, P., Panarese, V., Vincente, A. A., Wadsö, L., Finnie, C., Galindo, F. G. 2012. Effect of pulsed electric field on the germination of barley seeds. LWT-Food Science and Technology, vol. 47, no. 1, p. 161-166. https://doi.org/10.1016/j.lwt.2011.12.019

Freitas, I. R. A., Gananca, F., Santos, T. M., Carvalho, M. A. A., Motto, M., Vieira, M. C. 2005. Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira. Maydica, vol. 50, p. 105-112.

Gregova, E., Šliková, S., Hozlár, P. 2015. Seed protein electrophoresis for identification of oat registered cultivars. Potravinarstvo, vol. 9, no. 1, p. 411-416. https://doi.org/10.5219/513

Hamoud, M. A., El-Shanshory, A. R., Al-Sodany, Y. M., El-Karim, M. S. G. 2005. Genetic diversity among Ipomoea carnea jacq. Populations from different habitats types in Nile-Delta region of Egypt. The Egyptian Journal of Experimental Biology, vol. 1, p. 1-9.

Hanafy, M. S., Mohamed, H. A. and Abd El-Hady, E. A. 2006. Effect of low frequency electric field on growth characteristics and protein molecular structure of wheat plant. Romanian Journal of Biophysics, vol. 16, no. 4, p. 253-271.

Iqbal, J., Shinwari, Z. K., Rabbani, M. A., Khan, S. A. 2014. Genetic variability assessment of maize (Zea mays L.) germplasm based on total seed storage proteins banding pattern using SDS-PAGE. European academic research, vol. 2, no. 2, p. 2144-2160.

Iqbal, J., Shinwari, Z. K. and Rabbani, M. A. 2014. Investigation of total seed storage proteins of Pakistani and Japanese maize (Zea mays L.) through SDS-PAGE markers. Pakistan Journal of Botany, vol. 46, no. 3, p. 817-822.

Kačmárová, K., Lavová, B., Socha, P., Urminská, D. 2016. Characterization of protein fractions and antioxidant activity of Chia seeds (Salvia Hispanica L.). Potravinarstvo, vol. 10, no. 1, p. 78-82 https://doi.org/10.5219/563

Khan, A. H., Khan, N., Minhas, N. M., Ghafoor, A., Rabbani, M. A. 2014. Diversity in seed storage proteins in maize genetic resources: I. variation in alcohol soluble zein protein fraction. International Journal of Agricurtural and Biological Engineering, vol. 16, p. 1015-1018.

Larik, A. S. 1994. Genetic resources and their conservation in crop plants. In: Plant breeding. (Eds.): M. A. Khan, E. Bashir and R. Bantel. National Book Foundation, Islamabad, p. 235-254.

Lawton, J. W. 2006. Isolation of zein using 100% ethanol. Cereal Chemistry, vol. 83, no. 5, p. 565-568. https://doi.org/10.1094/CC-83-0565

Mabberely, D. J. 2008. Mabberleys Plant-Book: A portable dictionary of plants, their classifications, and uses. 3rd edition. Cambridge Univ. Press.: VII-XVIII, 1-1021.

Osman, G., Munshi, A., Altf, F. and Mutawie, H. 2013. Genetic variation and relationships of Zea mays and Sorghum species using RAPD-PCR and SDS-PAGE of seed proteins. African Journal of Biotechnology, vol. 12, no. 27, p. 4269-4276. https://doi.org/10.5897/ajb12.2644

Prasanna, B., Vasal, S., Kashun, B. and Singh, N. N. 2001. Quality protein maize. Current Science, vol. 81, no. 10, p. 1308-1319.

Ranjan, S., Poosapati, A., Vardhan, H. et al., 2013. Seed storage protein profile of few leguminous grains grown in india using SDSPAGE. International Journal of Advanced Biotechnology and Research, vol. 4, no. 4, p. 505-510.

Socha, P., Tomka, M., Kačmárová, K., Lavová, B., Ivanišová, E., Mickowska, B., Urminská, D. 2016. Comparable efficiency of different extraction protocols for wheat and rye prolamins. Potravinarstvo, vol. 10, no. 1, p. 139-144 https://doi.org/10.5219/540

Vasal, S. K., 1999. Quality protein maize story. Proceedings of workshop on improving human nutrition through agriculture: the role of international agricultural research. p: 1-16. IRRI, Los Banos, Philippines

Yoon, J. W., Jung, J. Y., Chung, H. J., Kim, M. R., Kim, Ch. W., Lim, S. T. 2010. Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins. Journal of Cereal Science, vol. 52, no. 2, p. 321-326. https://doi.org/10.1016/j.jcs.2010.06.015

Wrigley, C. W. 1992. Identification of cereal varieties by gel electrophoresis of the grain proteins. Heidelberg: Springer - Verlag, p. 17-41. https://doi.org/10.1007/978-3-662-01639-8_2

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Published

2016-11-16

How to Cite

Vivodí­k, M. ., Gálová, Z. ., Balážová, Želmí­ra ., Petrovičová, L. ., & Kuťka Hlozáková, T. . (2016). Genetic variation and relationships of old maize genotypes (Zea mays L.) detected using SDS-page. Potravinarstvo Slovak Journal of Food Sciences, 10(1), 532–536. https://doi.org/10.5219/661

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