Antibiotic resistance in bacteria Staphylococcus spp. isolated from samples of raw sheep's milk

Authors

  • Milan Vasiľ University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81, Košice
  • Juraj Elečko University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81, Košice
  • Zuzana Farkašová University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81, Košice
  • František Zigo University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81, Košice
  • Martin Lapin University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81, Košice

DOI:

https://doi.org/10.5219/680

Keywords:

sheep, raw milk, antibiotic, resistance, Staphylococcus spp.

Abstract

From samples of raw sheep's milk were determined results of bacteriological examination from two herds in region of Eastern Slovakia in three years lasting study. The occurrence of Staphylococcus spp. 41.6% (124) was determined from 298 samples. The seven species of staphylococci were on a regular basis isolated: S. epidermidis (34), S. chromogenes (26), S. aureus (16). Alternately have been recorded S. warneri (16), S. schleiferi (15), S. haemolyticus (9) and S. xylosus (8). All isolated pathogens were tested by in vitro test on Mueller-Hinton agar by disc methods on resistance to 10 types of antibiotics.  Highest value of resistance was determined to Penicilin 21.0%, Neomycin 10.5% and Novobiocin 9.7%. Lower resistance was in to Oxacilin 7.2% and Amoxicilin 6.5%. Minimal resistance was founded to Cefoxitin 0.8%, Linkomycin 2.4%, Erytromycin, and Streptomycin 3.2%. Was founded total resistance (21.0%) to all antibiotics in S. epidermidis (34) during the three years, S. chromogenes (26) showed resistance to 8 types of antibiotics (12.9%), S. aureus (16) to 6 antibiotics (10.5%) and S. warneri (16) to 4 antibiotics (5.6%). It was confirmed that sheep's milk remains a major source of staphylococci. Bacteria in comparison with isolates from cows' raw milk, showed lower values of resistance, but were resistant to more than two antibiotics. Recorded occurrence of resistance in staphylococci may be connected with a minimum use of antibiotics in the treatment of mastitis and other diseases in sheep herds. Reported resistance to the tested antibiotics became the basis for the recommendation to use preparations to treat mastitis in sheep principally by the detection of resistance to antibiotics contained.

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Published

2016-12-15

How to Cite

Vasiľ, M. ., Elečko, J. ., Farkašová, Z. ., Zigo, F. ., & Lapin, M. . (2016). Antibiotic resistance in bacteria Staphylococcus spp. isolated from samples of raw sheep’s milk. Potravinarstvo Slovak Journal of Food Sciences, 10(1), 619–624. https://doi.org/10.5219/680

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