Antibacterial activity of Capsicum extract against selected strains of bacteria and micromycetes

Authors

  • Peter Popelka Department of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice
  • Pavlí­na Jevinová Department of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice
  • Karel Šmejkal Veterinary and Pharmaceutical University in Brno, Faculty of Pharmacy, Department of Natural Drugs, Palackeho tr. 1946/1, 612 42 Brno
  • Pavol Roba Department of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice

DOI:

https://doi.org/10.5219/731

Keywords:

antibacterial activity, capsaicin, chilli peppers, HPLC, micromycetes

Abstract

One of traditional plant that has so many pharmacology effects is chilli fruit (Capsicum sp.) that belong to the family Solanaceae. Around the world is known five varieties of Capsicum which are C. annuum, C. frutescens, C. chinense, C. baccatum, and C. pubescens. Chilli peppers are known for causing the sensation of heat or burning when consumed. The heat sensation is incited by the type and the amount of a group of capsaicinoids, the alkaloids found only in chilli pepper pods. The HPLC method was used for determination of capsaicin and dihydrocapsaicin content in various dried peppers from genera C. chinense. Based on the results of HPLC the hottest pepper has been Bhut Jolokia, followed by Habanero Red Savina, Fatalii Yellow, Habanero Paper Latern, Habanero Maya Red, Habanero Red, and Scotch Bonnet Red. The inhibitory effect of the extract of Capsicum chinense pepper type was evaluated, using dics diffusion method, against selected bacteria and micromycetes. Extracts from Bhut Jolokia, Fatalii Yellow, Scotch Bonnet Red and habaneros did not inhibit growth of the any bacteria and micromycetes included in our test.

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References

Al Othman, Z. A., Ahmed, Y. B., Habila, M. A., Ghafar, A. A. 2011. Determination of Capsaicin and Dihydrocapsaicin in Capsicum Fruit Samples using High Performance Liquid Chromatography. Molecules, vol. 16, no. 10, p. 8919-8929. https://doi.org/10.3390/molecules16108919 PMid:22024959

Arora, R., Gill, N. S., Chauhan, G., Rana, A. C. 2011. An Overview about Versatile Molecule Capsaicin. A review article. International Journal of Pharmaceutical Science and Drug Research, vol. 3, no. 4, p. 280-286.

Bosland, P. W., Baral, J. B. 2007. Bhut Jolokia - The World's Hottest Known Chile Pepper is a Putative Naturally Occurring Interspecific Hybrid. HortScience, vol. 42, no. 2, p. 222-224.

Collins, M. D., Wasmund, L. M., Bosland, P. W. 1995. Improved method for quantifying capsaicinoids in Capsicum using high-performance liquid chromatography. Hort Science, vol. 30, no. 1, p.137-139.

Deal, C. L., Schnitzer, T. J., Lipstein, E., Seibold, J. R., Stevens, R. M., Levy, M. D., Albert, D., Renold, F. 1991. Treatment of arthritis with topical capsaicin: A double-blind trial. Clinical Therapeutics, vol. 13, no. 3, p. 383-395. PMid:1954640

Dorantes, L., Colmeneroa, R., Hernandeza, H., Motab, L., Jaramillo, M. E., Fernandez, E., Solano, C. 2000. Inhibition of growth of some foodborne pathogenic bacteria by Capsicum annum extracts. International Journal of Food Microbiology, vol. 57, no. 1-2, p. 125-128. https://doi.org/10.1016/S0168-1605(00)00216-6

Fattorusso, E., Taglialatela-Scafati, O. 2008. Modern alkaloids: structure, isolation, synthesis and biology. Weinheim : Wiley-VCH Verlag GmbH & Co. 689 p. ISBN: 978-3-527-31521-5.

Garces-Claver, A., Arnedo-Andres, M. S., Abadia, J., Gil-Ortega, R., Alvarez-Fernandez, A. 2006. Determination of Capsaicin and Dihydrocapsaicin in Capsicum Fruits by Liquid Chromatography-Electrospray/Time-of-Flight Mass Spectrometry. Journal of Agricultural and Food Chemistry, vol. 54, no. 25, p. 9303-9311. https://doi.org/10.1021/jf0620261 PMid:17147411

Harvell, K. P., Bosland, P. W. 1997. The environment produces a significant effect on pungency of chiles (Capsicum annuum L.). HortScience, vol. 32, no. 7, p. 1292.

Holzer, P. 1991. Capsaicin: cellular targets, mechanisms of action, and selectivity for thin sensory neurons. Pharmacological Reviews, vol. 43, no. 2, p. 143-201. PMid:1852779

Kempaiah, R. K., Manjunatha, H., Srinivasan, K. 2005. Protective effect of dietary capsaicin on induced oxidation of low-density lipoprotein in rats. Molecular and Cellular Biochemistry, vol. 275, no. 1-2, p. 7-13. https://doi.org/10.1007/s11010-005-7643-3 PMid:16335782

Kim, K., Ryeom, K. 1979. A study on content and antibacterial effects of capsaicin from Korean hot pepper. Report of the National Institute of Health, vol. 16, 241-251.

Kim, J. M., Marshall, M. R., Cornell, J. A., Preston III, I. F., Wei, C. I. 1995. Antibacterial activity of carvacrol, citral and geraniol against Salmonella typhimurium in culture medium and on fish cubes. Journal of Food Science, vol. 60, no. 6, p. 1364-1368. https://doi.org/10.1111/j.1365-2621.1995.tb04592.x

Lopez-Hernandez, J., Oruna-Concha, M. J., Simal-Lozano, J., Gonzales-Castro, M. J., Vazquez-Blanco, M. E. 1996. Determination of capsaicin and dihydrocapsaicin in cayenne pepper and padron peppers by HPLC. Deutsche Lebensmittel Rundschau, vol. 92, p. 393-395.

López-Malo, A., Alzamora, S. M., Argais, A. 1998. Vanillin and pH synergistic effects on mould growth. Journal of Food Science, vol. 63, no. 1, p. 143-146. https://doi.org/10.1111/j.1365-2621.1998.tb15695.x

Morré, D. J., Morré, D. M. 2003. Synergistic Capsicum-tea mixtures with anticancer activity. Journal of Pharmacy and Pharmacology, vol. 55, no. 7, p. 987-994. https://doi.org/10.1211/0022357021521 PMid:12906756

Nascimento, P. L. A., Nascimento, T. C. E. S., Ramos, N. S. M., Silva, G. R., Galindo Gomes, J. E., Falcão, R. E. A., Moreira, K. A., Porto, A. L. F., Silva, T. M. S. 2014. Quantification, Antioxidant and Antimicrobial Activity of Phenolics Isolated from Different Extracts of Capsicum frutescens (Pimenta Malagueta). Molecules, vol. 19, no. 4, p. 5434-5447. https://doi.org/10.3390/molecules19045434 PMid:24879587

Parrish, M. 1996. Liquid chromatographic method of determining capsaicinoids in capsicums and their extractives: collaborative study. Journal of AOAC International, vol. 79, no. 3, p. 738-745.

Perucka, I., Oleszek, W. 2000. Extraction and determination of capsaicinoids in fruit of hot pepper Capsicum annuum L. by spectrophotometry and high-performance liquid chromatography. Food Chemistry, vol. 71, no. 2, p. 287-291. https://doi.org/10.1016/S0308-8146(00)00153-9

Scoville, W. L., 1912: Note on Capsicum. Journal of the American Pharmaceutical Association, vol. 1, no. 5, p. 453-454. https://doi.org/10.1002/jps.3080010520

Serruti, P., Alzamora, S. M. 1996. Inhibitory effects of vanillin on some food spoilage yeast in laboratory media and fruit purees. International Journal of Food Microbiology, vol. 29, no. 2-3, p. 379-386. https://doi.org/10.1016/0168-1605(95)00026-7

Szolcsanyi, J. 2004. Forty years in capsaicin research for sensory pharmacology and physiology. Neuropeptides, vol. 38, no. 6, p. 377-384. https://doi.org/10.1016/j.npep.2004.07.005 PMid:15567473

Thomas, B. V., Schreiber, A. A., Weisskopf, C. P. 1998. Simple method for quantitation of capsaicinoids in peppers using capillary gas chromatography. Journal of Agricultural and Food Chemistry, vol. 46, no. 7, p. 2655-2663. https://doi.org/10.1021/jf970695w

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Published

2017-04-19

How to Cite

Popelka, P. ., Jevinová, P. ., Šmejkal, K. ., & Roba, P. . (2017). Antibacterial activity of Capsicum extract against selected strains of bacteria and micromycetes. Potravinarstvo Slovak Journal of Food Sciences, 11(1), 223–229. https://doi.org/10.5219/731

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