Effect of apricot seeds on renal structure of rabbits

Authors

  • Anna Kolesárová Slovak University of Agriculture in Nitra, Department of Storing and Processing of Plant Products, Faculty of Biotechnology and Food Sciences, Tr. A. Hlinku 2, 949 76 Nitra
  • Juraj Pivko Animal Production Research Centre Nitra, National Agricultural and Food Center, Hlohovecká 2, 951 41 Lužianky
  • Marek Halenár Slovak University of Agriculture in Nitra, Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Tr. A. Hlinku 2, 949 76 Nitra
  • Katarí­na Zbyňovská Slovak University of Agriculture in Nitra, Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Tr. A. Hlinku 2, 949 76 Nitra
  • Ľubica Chrastinová Animal Production Research Centre Nitra, National Agricultural and Food Center, Hlohovecká 2, 951 41 Lužianky
  • Ľubomí­r Ondruška Animal Production Research Centre Nitra, National Agricultural and Food Center, Hlohovecká 2, 951 41 Lužianky
  • Rastislav Jurčí­k Animal Production Research Centre Nitra, National Agricultural and Food Center, Hlohovecká 2, 951 41 Lužianky
  • Jana Kopčeková Slovak University of Agriculture in Nitra, Department of Human Nutrition, Faculty of Agrobiology and Food Resources, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Valuch Health Care Surveillance Authority, Žellova 2, 829 24 Bratislava
  • Adriana Kolesárová Slovak University of Agriculture in Nitra, Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/751

Keywords:

seeds, amygdalin, rabbits, kidney

Abstract

Amygdalin is the major cyanogenic glycoside present in apricot seeds and is degraded to cyanide by chewing or grinding. The animal data available did not provide a suitable basis for acute human health hazard. The apricot seeds are potentially useful in human nutrition and for treatment of several diseases especially cancer. The present study demonstrates the potential effect of short-term oral application of apricot seeds on renal structure of rabbit as a biological model. Meat line P91 Californian rabbits from the experimental farm of the Animal Production Research Centre Nitra (Slovak Republic) were used in the experiments. The animals were randomly divided into the three groups (C-control, P1, P2 - experimental groups) leading to 8 rabbits in each group. The control group received no apricot seeds while the experimental groups P1 and P2 received a daily dose 60 and 300 mg.kg-1 b.w. of crushed apricot seeds mixed with feed during 28 days, respectively. After 28 days all animals were slaughtered and kidney tissue was processed by standard histopathological techniques. Tissue sections were observed under an optical microscope with camera Olympus CX41 (Olympus, Japan) at a magnification of 10 x 0.40. The basic morphometric criteria of the preparations were quantified using image program MeasurIT (Olympus, Japan). From each sample (n = 24) three histological sections with five different fields of view in each section were analysed and followed parameters were analysed: diameter of renal corpuscles (RC), diameter of glomeruli (G), diameter of tubules (T) and the height of epithelial tubules (E). In our study, we observed a slight increase in the most frequent occurrence parenchyma dystrophy experimental animals. These changes were more pronounced in the experimental group (P2) rabbits received a daily dose of 300 mg.kg-1 of body weight of apricot seeds. Most often, we have found enlarged glomeruli filling the entire space of the capsule, and also glomerular basement membrane thickening. The most frequent alterations of tubular organs manifested by thickening and dilatation of proximal tubules and in the lumen of the occurrence fuchsinophilic mass, grains and hyaline cylinders. The occurrence of the vacuole and parenchymal atrophy was mostly balanced groups. Changes in P2 group are also reflected in morphometric evaluation structures. We have found significant decrease (p <0.001) in the average of all renal structures (diameter of renal corpuscles, diameter of glomeruli, diameter of tubules, and the height of epithelial tubules). Inversely, oral administration a daily dose of 60 mg.kg-1 of body weight of apricot seeds had no significant impact on these parameters. The change displays only the increase of renal tubule diameter. Our data may provide more specific evidence of oral application of apricot seeds on renal structure but further detailed studies are also required.

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References

Aunapuu, M., Pechter, U., Kühnel, W., Ots, M., Arend, A. 2005. Morphological changes in experimental postischemic rat kidney. A pilot study. Annals of Anatomy - Anatomischer Anzeiger, vol. 187, no 1, 2005, p. 63-70. https://doi.org/10.1016/j.aanat.2004.07.002 PMid:15835402

Balmer, C. B. 1998. Alternative therapies in cancer patient care. Highlights in oncology practice, vol. 15, p. 83-84.

Bensky, D., Clavey, E., Stöger, E. 2004. Chinese Herbal Medicine: Materia Medica. 3rd ed. Seattle : Eastland Press, p. 437-440. ISBN-13: 978-0939616824.

EPA, 1990. Summary Review of Health Effects Associated with Hydrogen Cyanide, Health Issue Assessment Environmental Criteria and Assessment Office, Office of Health and Environmental Assessment Office of Research and Development, US Environmental Protection Agency Research Triangle Park, North Carolina, USA.

Freese, A., Brady, R. O., Gal, A. E., 1980. A beta-glucosidase in feline kidney that hydrolyzes amygdalin (Laetrile). Archives of Biochemistry and Biophysics, vol. 201, no. 2, p. 363-368. https://doi.org/10.1016/0003-9861(80)90523-8

Gomez, E., Burgos, L., Soriano, C. 1998. Amygdalin content in the seeds of several apricot cultivars. Journal of the Science of Food and Agriculture, vol. 77, no. 2, p. 184-186. https://doi.org/10.1002/(SICI)1097-0010(199806)77:2<184::AID-JSFA22>3.0.CO;2-H

Hakan, P., Ercument, O., Ahmet, A., Feral, O., Seda, T., Burhan, A., Mehmet, G., Ali, O. 2009. Beneficial effects of apricot-feeding on myocardial ischemia-reperfusion injury in rats. Food and Chemical Toxicology, vol. 47, no. 4, p. 802-808. https://doi.org/10.1016/j.fct.2009.01.014

Hayes, W. J. 1967. The 90 day LD50 and chronicity factors as a measure of toxicity. Toxicology and Applied Pharmacology, vol. 11, no. 2, p. 327-335. https://doi.org/10.1016/0041-008X(67)90076-2

IPCS, 1992. Poison Information Monograph - Cyanides. Geneva, World Health Organization, International Programme on Chemical Safety, September (IPCS/INTOX/PIM 159).

JECFA, 1993. Cyanogenic glycosides. In: Toxicological evaluation of certain food additives and naturally occurring toxicants. Geneva : World Health Organization, 49 p.

Jelínek, P., Koudela, K. 2003. Fyziologie hospodářskych zvířat (Animal physiology). Brno : Mendelova zemnědelská a lesnická univerzita v Brne, p. 208-219. ISBN 80-7157-644-1.

Kováčová, V., Omelka, R., Šarocká, A., Šranko, P., Adamkovičová, M., Toman, R., Halenár, M., Kolesárová, A., Martiniaková, M. 2016. Histological analysis of femoral bones in rabbits administered by amygdalin. Potravinarstvo, vol. 10, no. 1, p. 393-399. https://doi.org/10.5219/625

Lasch, E. E, El Shawa, R. 1981. Multiple cases of cyanide poisoning by apricot kernels in children from Gaza. Pediatrics, vol. 68, no. 1, p. 5-7.

Lee. J., Zhang, G., Wood, E., Castillo. C., Mitchell, A. E. 2013. Quantification of amygdalin in nonbitter, semibitter, and bitter almonds (Prunus dulcis) by UHPLC-(ESI)QqQ MS/MS. Journal of Agricultural and Food Chemistry, vol. 61, no. 32, p. 7754-7759. https://doi.org/10.1021/jf402295u PMid:23862656

Leuschner, F., Neumann, B. W., 1989. 13-Week toxicity study of potassium cyanide administered to Sprague-Dawley rats in the drinking water: Unpublished study. Laboratory of Pharmacology and Toxicology.

Levey, A. S., Coresh, J. 2012. Chronic kidney disease. The Lancet. vol. 379, no. 9811, p. 165-180. https://doi.org/10.1016/S0140-6736(11)60178-5

Liu, Y. 2016 Renal fibrosis: New insights into the pathogenesis and therapeutics. Kidney International, vol. 69, no. 2, p. 213-217. https://doi.org/10.1038/sj.ki.5000054 PMid:16408108

Lukáč, N., Massányi, P., Toman, R., Trandžík, J., Capcarová, M., Stawarz, R., Cigánková, V., Jakabová, D., Kožáková, I., Forgács, Z., Somosy, Z. 2006. Prejavy toxicity olova a ortuti. In Kováčik, J., et al.: Biologické aspekty zvyšovania kvality surovín a potravín živočíšneho pôvodu: Vedecká monografia (Biological aspects of improving the quality of raw materials and food of animal origin: Scientific monograph). Nitra : SPU, p. 145-155.

Mahjour, M., Khoushabi, A., Novi, M. M. G., Feyzabadi Z. 2017. Food strategies of renal atrophy based on Avicenna and conventional medicine. Journal of Traditional and Complementary Medicine, In Press, Corrected Proof. https://doi.org/10.1016/j.jtcme.2016.12.004

OKE, O. L. 1979. Some aspects of the role of cyanogenic glycosides in nutrition. World Review of Nutrition and Dietetics, vol. 33, p. 70-103. https://doi.org/10.1159/000402550

Padmaja, G. 1995. Cyanide detoxification in cassava for food and feed use. Critical Reviews in Food Sciences and Nutrition, vol. 35, no. 4, p. 259-339. https://doi.org/10.1080/10408399509527703

PMid:7576161

Panel on Contaminants in the Food Chain. 2016. Acute health risks related to the presence of cyanogenic glycosides in raw apricot kernels and products derived from raw apricot kernels. EFSA Journal; vol. 14, no.4, p. 47.

Solomonson, L. P. 1981. Cyanide as a metabolic inhibitor. In Vennesland, B. Cyanide in Biology. Michigen : Academic Press, p. 11-18. ISBN 0127169806.

Song, Z., Xu, X. 2014. Advanced research on anti-tumor effects of amygdalin. Journal of Cancer Research & Therapy, vol. 1, p. 3-7.

Sousa, A. B, Soto-Blanco, B., Guerra, J. L, Kimura, E. T., Gorniak, S. L. 2002. Does prolonged oral exposure to cyanide promote hepatotoxicity and nephrotoxicity? Toxicology, vol. 174, no. 2, p. 87-95. https://doi.org/10.1016/S0300-483X(02)00041-0

Thomas,, M. C., Burns, W. C., Cooper, M. E. 2005. Tubular changes in early diabetic nephropathy. Advances in Chronic Kidney Disease, vol. 12, no. 2, 2005, p. 177-186. https://doi.org/10.1053/j.ackd.2005.01.008

PMid:15822053

Trojan, S. 1992. Fyziológia 1 (Physiology 1). Martin : Osveta, p.411. ISBN 80-217-0452-7.

Tulsawani, R. K., Debnath, M., Pant, S. C., Kumar, O., Prakash, A. O., Vijayaraghavan, R., Bhattacharya, R. 2005. Effect of sub-acute oral cyanide administration in rats: protective efficacy of alpha-ketoglutarate and sodium thiosulfate. Chemico-Biological Interactions, vol. 156, no. 1, p. 1-12. https://doi.org/10.1016/j.cbi.2005.05.001 PMid:16154552

US EPA (US Environmental Protection Agency), 2010. Toxicological review of hydrogen cyanide and cyanide salts (CAS No. various) in support of summary information on the Integrated Risk Information System (IRIS). Environmental Protection Agency, Washington, DC, USA.

Yamaguchi, T., Yamamoto, K., Asano, Y., 2014. Identification and characterization of CYP79D16 and CYP71AN24 catalyzing the first and second steps in L-phenylalanine-derived cyanogenic glycoside biosynthesis in the Japanese apricot, Prunus mume Sieb. et Zucc. Plant Molecular Biology, vol. 86, p. 215-223. https://doi.org/10.1007/s11103-014-0225-6

PMid:25015725

Zhang, J., Gu, H. D., Zhang, L., Tian, Z. J., Zhang, Z. Q., Shi, X. C., Ma, W. H. 2011. Protective effects of apricot kernel oil on myocardium against ischemia-reperfusion injury in rats. Food and Chemical Toxicology, vol. 49, no. 12, p. 3136-3141. https://doi.org/10.1016/j.fct.2011.08.015

PMid:21896302

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Published

2017-05-16

How to Cite

Kolesárová, A. ., Pivko, J. ., Halenár, M. ., Zbyňovská, K. ., Chrastinová, Ľubica ., Ondruška, Ľubomí­r ., Jurčí­k, R. ., Kopčeková, J. ., Valuch, J. ., & Kolesárová, A. . (2017). Effect of apricot seeds on renal structure of rabbits. Potravinarstvo Slovak Journal of Food Sciences, 11(1), 309–314. https://doi.org/10.5219/751

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