Evaluation of rabbit meat microbiota from the viewpoint of marketing method

Authors

  • Olga Cwiková Mendel University in Brno, Faculty of AgriSciences, Department of Food Technology, Zemědělská 1, 61300 Brno
  • Roman Pytel Mendel University in Brno, Faculty of AgriSciences, Department of Food Technology, Zemědělská 1, 613 00 Brno

DOI:

https://doi.org/10.5219/764

Keywords:

rabbit, TAC, LAB, Enterobacteriaceae, psychrotrophic microorganisms, yeasts and moulds

Abstract

Microbiological analysis was performed on carcasses of rabbits coming from domestic slaughter, purchased at butcher shops, vacuum-packaged and purchased in supermarkets, as well as frozen. The total number of analysed rabbits was 20. For all samples the following microbiological parameters were determined: total microorganisms count (TAC), the count of lactic acid bacteria (LAB), psychrotropic microorganisms, moulds and yeasts, as well as bacteria of the Enterobacteriaceae family. Total microorganisms count was the highest (p <0.05) in rabbit meat from butcher shops (5.34 log CFU.g-1). The counts of lactic acid bacteria (LAB) in rabbit meat originating from domestic breeding was 2.58 log CFU.g-1, in vacuum-packaged rabbits 3.18 log CFU.g-1, in frozen rabbits 2.29 log CFU.g-1, and in rabbit meat purchased from butcher shops 3.58 log CFU.g-1. The highest count (p <0.05) of Enterobacteriaceae was observed in samples from butcher shops, namely 2.91 log CFU.g-1. In contrast the lowest count (p <0.05) was in rabbit meat from home slaughtering at 1.47 log CFU.g-1 and in frozen ones at 1.36 log CFU.g-1. The lowest counts (p <0.05) of moulds and yeasts were observed in rabbit meat from domestic slaughter, namely 1.12 log CFU.g-1. The highest counts (p <0.05) were in rabbit meat from butcher shops 2.97 log CFU.g-1. The highest counts (p <0.05) of psychrotrophic microorganisms were detected in rabbit meat from butcher shops, namely 4.98 log CFU g-1 and the lowest ones (p <0.05) in the meat of domestically slaughtered rabbits at 2.52 log CFU.g-1. In all monitored microbiological indicators, we have found differences (p <0.05) in their counts on the surface and inside the muscle tissue, both on the front and rear parts of the rabbit carcass

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References

Abdel-Rahman, H. A., Yassein, M. A., Ahmed, A. M., Hayashidani, H., Elhelaly, A. E. 2008. Bacterial profile of frozen broiler chickens. Suez Canal Veterinary Medicine Journal, vol. 13, no. 1, p. 49-60.

Badr, H. M. 2004. Use of irradiation to control foodborne pathogens and extend the refrigerated market life of rabbit meat. Meat Science, vol. 67, no. 4, p. 541-548. https://doi.org/10.1016/j.meatsci.2003.11.018 PMid:22061802

Berruga, M. I., Vergara, H., Linares, M. B. 2005. Control of microbial growth and rancidity in rabbit carcasses by modified atmosphere packaging. Journal of the Science of Food and Agriculture, vol. 85, no. 12, p. 1987-1991. https://doi.org/10.1002/jsfa.2183

Corry, L. E. L. 2007. Spoilage organisms of red meat and poultry and eggs. In Mead, G. Microbial Analysis of Red Meat, poultry and Eggs. Sawston, Cambridge, UK : Woodhead Publishing p. 101-122. ISBN 978-1-84569-059-5. https://doi.org/10.1533/9781845692513.101

Decree no. 132/2004 Sb. Ministry of Health of the Czech republic on the microbiological requirements for foods, to their monitoring and evaluation. Collection of Laws, No. 42, 03/26/2004, p. 1738-1757. (Vyhláška Ministerstva zdravotnictví o mikrobiologických požadavcích na potraviny, způsobu jejich kontroly a hodnocení. Sbírka zákonů ČR, částka 42, 26/3/2004, p. 1738-1757.

Chabela, M. L. P., Serrano, G. M. R., Calderon, P. L., Guerrero, I. 1999. Microbial spoilage of meats offered for retail sale in Mexico City. Meat Science, vol. 51, no. 4, p. 279-282. https://doi.org/10.1016/S0309-1740(98)00096-5

Görner, F., Valík, L. 2004. Applied food microbiology. (Aplikovaná mikrobiológia poživatín). Bratislava, Slovakia : Malé Centrum, PPA. 528 p. ISBN 80-967064-9-7.

Hernández, P. 2008. Enhancement of nutritional quality and safety in rabbit meat. In: Proceedings of 9th World Rabbit Congress. Verona, Italy, p. 1287-1299.

ISO 13721:1998. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of mesophilic lactic acid bacteria Colony-count technique at 30 degrees.

ISO 17410:2003. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of psychrotrophic microorganisms.

ISO 6887-2:2003. Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 2: Specific rules for the preparation of meat and meat products.

ISO 21528-2:2006. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of Enterobacteriaceae, Colony-count technique.

ISO 7218:2007. Microbiology of food and animal feeding stuffs-General requirements and guidance for microbiological examinations.

ISO 21527-1:2009. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of yeasts and moulds - Part 1: Colony count technique in products with water activity greater than 0.95.

ISO 4833-1:2014. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of micro-organisms, Colony-count technique at 30 °C.

Koutsoumanis, K., Sofos, J. N. 2004. Microbial contamination W.K. in Devine, C. et al. Encyclopedia of meat sciences. 2nd ed. UK : Elsevier, p. 727-737. ISBN: 978-0-12-464970-5. https://doi.org/10.1016/B0-12-464970-X/00070-2

Kameník, J., Chomát, P. 2013. „B" as a package of meat and meat products („B" jako balení masa a masných výrobků). Maso, vol. 24, no. 1, p. 8-13.

Kohler, R., Krause, G., Bentin, L., Stephan, R., Zweifel, C. 2008. Shedding of food-borne pathogens and microbiological carcass contamination in rabbits at slaughter. Veterinary microbiology, vol. 132, no. 1-2, p. 149-157. https://doi.org/10.1016/j.vetmic.2008.04.020 PMid:18514438

Lan, Y., Shang, Y., Song, Y., Dong, Q. 2016. Changes in the quality of superchilled rabbit meat stored at different temperatures. Meat Science, vol. 117, p. 173-181. https://doi.org/10.1016/j.meatsci.2016.02.017 PMid:26990070

Ludewig, M., Fehlhaber, K. 2005. Investigation on the microbiological status of rabbit meat with respect to bacteria relevant to food hygiene. Archiv fur Lebensmittelhygiene, vol. 56, no. 2, p. 28-32.

Nakyinsigea, K., Sazilia, A. Q., Aghwana, Z. A., Zulkiflia, I., Gohc, Y. M., Abu Bakara, F., Saraha, S. A. 2015. Development of microbial spoilage and lipid and protein oxidation in rabbit meat. Meat Science, vol. 108, p. 125-131. https://doi.org/10.1016/j.meatsci.2015.05.029 PMid:26115345

Pereira, M., Malfeito-Ferreira, M. 2015. A simple method to evaluate the shelf life of refrigerated rabbit meat. Food Control, vol. 49, p. 70-74. https://doi.org/10.1016/j.foodcont.2013.10.021

Rodríguez-Calleja, J. M., Santos, J. A., Otero, A. 2004. Microbiological quality of rabbit meat. Journal of Food protection, vol. 67, no. 5, p. 966-971. https://doi.org/10.4315/0362-028X-67.5.966 PMid:15151235

Rodríguez-Calleja, J. M., García-López, M. L., Santos, J. A., Otero, A. 2005. Development of the aerobic spoilage flora of chilled rabbit meat. Meat Science, vol. 70, no. 2, p. 389-394. https://doi.org/10.1016/j.meatsci.2005.01.009 PMid:22063497

Rodríguez-Calleja, J. M., García-López, M. L., Santos, J. A., Otero, A. 2006. Rabbit meat as a source of bacterial foodborne pathogens. Journal of Food Protection, vol. 69, no. 5, p. 1106-1112. https://doi.org/10.4315/0362-028X-69.5.1106 PMid:16715811

Rodríguez-Calleja, J. M., Santos, J. A., Otero, A., García-López, M. L. 2010. Effect of vacuum and modified atmosphere packaging on the shelf life of rabbit meat. Cyta - Journal of Food, vol. 8, no. 2, p. 109-116. https://doi.org/10.1080/19476330903205041

Šilhánková, L. 2008. Microbiology for food and biotechnology workers (Mikrobiologie pro potravináře a biotechnology). Praha, Czech Republic : Nakladatelství Academia, 363 p., ISBN 8-85605-71-6.

Steinhauser, L. et al. 2000. Hygiene and technology of meat (Hygiena a technologie masa), Brno, Czech Republic : LAST, 643 p. ISBN 80-900260-7-9.

Szkucik, K., Pyz-Łukasik, R. 2009. Quality health of rabbit meat (Jakośc´ zdrowotna miesa królików). Medycyna Weterynaryjna, vol. 65, no. 10, p. 665-669.

Vannini, L., Sado, S., Iucci, L., Ndagijimana, M., Guerzoni, M. E. 2003. The dietary use of linseed in growing rabbits: effects on microbial population and spoilage patterns of meat products. In: Proceedings of 3rd Meeting Working Group 4 "Nutrition and pathology" and 5 "Meat quality and safety". Praha : Czech Republic, p. 32-33.

Vlková, E., Rada, V. Killer, J. 2009. Food microbiology (Potravinářská mikrobiologie). Praha, Czech Republic : Česká zemědělská univerzita v Praze, 168 p. ISBN 978-80-213-1988-2.

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Published

2017-06-15

How to Cite

Cwiková, O. ., & Pytel, R. . (2017). Evaluation of rabbit meat microbiota from the viewpoint of marketing method. Potravinarstvo Slovak Journal of Food Sciences, 11(1), 391–397. https://doi.org/10.5219/764

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