Effect of selected polymorphisms of genes lep, MTHFR and FTO to BMI level and gender-specificity

Authors

  • Kristí­na Candráková Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Animal Breeding Biology, Tr. A. Hlinku 2, 949 76 Nitra
  • Anna Trakovická Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Animal Breeding Biology, Tr. A. Hlinku 2, 949 76 Nitra
  • Michal Gábor Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Animal Breeding Biology, Tr. A. Hlinku 2, 949 76 Nitra
  • Martina Miluchová Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Animal Breeding Biology, Tr. A. Hlinku 2, 949 76 Nitra
  • Radovan Kasarda Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Animal Breeding Biology, Tr. A. Hlinku 2, 949 76 Nitra
  • Nina Moravčí­ková Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Animal Breeding Biology, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/834

Keywords:

FTO rs1121980, LEP G2548A, MTHFR C677T, BMI, gender-specificity

Abstract

The aim of this study is to investigate the effect of selected polymorphisms LEP (G2548A), MTHFR (C677T) and FTO (rs1121980) on body mass index in humans. In the study participated 79 people from Slovakia with some genetic relatedness. Genomic DNA was isolated from the buccal swabs using a commercial kit Qiagen DNA Mini Kit (Qiagen). The detection of SNP polymorphisms in human genes LEP, MTHFR and FTO was performed using molecular genetics methods such as PCR-RFLP and ARMS. The most common genotypes in all 3 polymorphism were found in heterozygous form (for LEP AG = 0.5190, for MTHFR CT = 0.519, for FTO CT =0.4051). The LEP gene had increased frequency of G allele (0.5506), the MTHFR gene T allele (0.6646) and FTO gene T allele (0.50635). The least frequent genotype in LEP was AA (0.1899), in MTHFR was TT (0.0759), in FTO it was CC (0.2911). According to the results we can assume that the genotypes AA (LEP G2548A), CC (FTO rs1121980) and TT (MTHFR C677T) in case of women have a protective effect on the prevalence of obesity, based on BMI, compared to the other genotypes and this polymorphism is gender-specific. Added anthropometric measurements, blood test and extension of the group in the future evaluation could increase the statistical relevance in relation to obesity and gender-specificity.

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References

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Published

2017-12-13

How to Cite

Candráková, K. ., Trakovická, A. ., Gábor, M. ., Miluchová, M. ., Kasarda, R. ., & Moravčí­ková, N. . (2017). Effect of selected polymorphisms of genes lep, MTHFR and FTO to BMI level and gender-specificity. Potravinarstvo Slovak Journal of Food Sciences, 11(1), 747–753. https://doi.org/10.5219/834

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