Characterization of Escherichia coli strains isolated from raw vegetables

Authors

  • Pavel Pleva Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlí­n, Vavrečkova 275, 760 01 Zlí­n
  • Magda Janalí­ková Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlí­n, Vavrečkova 275, 760 01 Zlí­n
  • Silvie Pavlí­čková Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlí­n, Vavrečkova 275, 760 01 Zlí­n
  • Martin Lecomte Institute Universitaire de Technologie, Université d'Angers, 40, rue de Rennes - BP 73532, 49035 - Angers cedex 01
  • Tanguy Godillon Institute Universitaire de Technologie, Université d'Angers, 40, rue de Rennes - BP 73532, 49035 - Angers cedex 01
  • Ivan Holko Vetservis s.r.o., Kalvária 715/3, 949 01 Nitra

DOI:

https://doi.org/10.5219/897

Keywords:

vegetables, Escherichia coli, virulence, resistance, safety

Abstract

Vegetables are an important part of the human diet. Sometimes, contamination by pathogenic Escherichia coli can be underestimated; moreover there is a risk of antibiotic resistance spreading via the food chain. The purpose of this study was to examine the prevalence of Escherichia coli in fresh vegetables sold in retail market in the Czech Republic and to evaluate the risk to human health. Antibiotic resistance against 12 antibiotics, the presence of 12 virulence and 15 resistance genes were determined among 15 isolated strains. Most of tested strains belonged to B1 phylogenetic group, less frequently represented was B2 and D phylogroup. These results indicate that most strains are probably of human origin. All E. coli strains were resistant to at least one of twelve tested antibiotics. A multidrug resistance was observed in four strains. In this study, the presence of virulence factors Einv and papC and also genes encoding toxins (CNF1, CNF2) was detected. Nevertheless, none strain can be considered as STEC or EHEC. The widespread appearance of a growing trend associated with the prevalence of antibiotic resistance among enterobacterial isolates is undeniable and the possibility of transfer to humans cannot be ignored. Nevertheless, these results indicate that raw vegetables sold in the retail market can constitute a potential health risk for consumers.

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Published

2018-03-27

How to Cite

Pleva, P. ., Janalí­ková, M. ., Pavlí­čková, S. ., Lecomte, M. ., Godillon, T. ., & Holko, I. . (2018). Characterization of Escherichia coli strains isolated from raw vegetables. Potravinarstvo Slovak Journal of Food Sciences, 12(1), 304–312. https://doi.org/10.5219/897

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