Development of resistance to antibiotics in bacteria Staphylococcus spp. Isolated from milk samples in the sheep breedings on east of Slovakia

Authors

  • Milan Vasiľ University of veterinary medicine and pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81 Košice
  • Juraj Elečko University of veterinary medicine and pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81 Košice
  • Zuzana Farkašová University of veterinary medicine and pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81 Košice
  • František Zigo University of veterinary medicine and pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81 Košice

DOI:

https://doi.org/10.5219/904

Keywords:

sheep milk, staphylococci, antibiotics, resistance, mastitis

Abstract

During the last three years (2015 to 2017), the frequency of occurrence of bacteria Staphylococcus spp. were examined in total 3466 individual and 12 pool milk samples. Experiment was carried out in two herds of breed of sheep Improved Valaska in region of East Slovakia. Were isolated and taxonomically identified 15 species of the genus Staphylococcus spp. (n = 444). From the coagulase positive staphylococci (CPS), S. aureus was isolated during the reporting period, however, most often in the first year (45). The incidence of S. intermedius and S. hyicus has been irregular. From the coagulase negative staphylococci (CNS) (n = 288), were isolated S. epidermidis present in 37.5% (108), S. scheiferi 25.69% (74) and S. chromogenes 23.61% (68), while other species occurred only rarely. The bacteria S. aureus (n = 117) showed the highest resistance to novobiocine 14.5%, to erythromycin 12.8%, lincomycin 7.69% and also 7.69% to penicillin. In the framework of the CNS sensitivity we tested 108 strains of S. epidermidis, from which it was 11.1% resistant to novobiocine and 8.3% to erythromycin. Statistical comparison of the incidence of resistance to penicillin and novobiocine in S. aureus and S. epidermidis was performed using Chi square test. There was statistically significant dependence of tracked characters was confirmed on significance level α = 0.05. Also, the incidence of intermediate sensitivity of bacteria in the Staphylococcus spp., indicates the unfavourable development of resistance to the most commonly used antibiotics to treat the inflammation of the udder in sheep. It is therefore recommended to regularly check the resistance to antibiotics and often isolated bacteria CNS.

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References

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Published

2018-03-16

How to Cite

Vasiľ, M. ., Elečko, J. ., Farkašová, Z. ., & Zigo, F. . (2018). Development of resistance to antibiotics in bacteria Staphylococcus spp. Isolated from milk samples in the sheep breedings on east of Slovakia. Potravinarstvo Slovak Journal of Food Sciences, 12(1), 273–278. https://doi.org/10.5219/904

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