Mastitis pathogens and their resistance against antimicrobial agents in herds of dairy cows situated in marginal parts of Slovakia
Keywords:dairy cows, mastitis, resistance, Staphylococcus aureus, Streptococcus agalactiae
AbstractMarginal regions are relatively large part of the area Slovakia which in terms of the economy breeding ruminants can efficiently produce animal commodities only occasionally. Geographic, social and economic stability of these regions is strongly influenced by breeding of ruminants with market milk production. Mastitis is a disease complex that assumes highest clinical and economic significance in milk animals particularly medium to high yielding dairy cattle, usually in and around periparturient period. The objective of this study was to evaluate the effectiveness of different antibiotics against mastitis causing microorganisms during first month of lactation in two herds of 230 and 310 dairy cows situated in marginal parts of Slovakia. Milk samples from quarters were cultured and identified bacteria were subjected to antimicrobial susceptibility test by disc diffusion method to a large number of antibiotics. The prevalence of mastitis in the monitored herds of dairy cows was 26.1% to 17.6%, respectively. A total of 1663 milk samples from udder quarters were investigated, 446 (21.3%) samples were positive. No pathogens were isolated from 1663 (78.4%) milk samples. From all tested bacteria Staphylococcus spp. and Streptococcus spp. which were isolated from subclinical and clinical mastitis, were found amoxicillin + clavulanat and tetradelta to be most effective drug followed by ceftiofur and rifaximin. The significant difference was confirmed between the Staph. aureus and coagulase-negative staphylococci (CoNS) isolates with respect to their susceptibility to the various antibiotics. Antibiotic susceptibility tests should be done to determine the effectiveness of drug that can be used for successful treatment of diseases. Proper isolation and identification of the causative organism play significant role in prevention and control of the diseases.
Allore, H. G., Erb, H. N., Schruben, L. W., Oltenacu, P. A., 1998. A simulation of strategies to lower tank somatic cell count below 500 000 per mililiter, Jour. Dairy Sci., vol 81, no. 3, p. 694-702. https://doi.org/10.3168/jds.S0022-0302(98)75625-5
Aarestrup, F. M. 2005. Veterinary drug usage and antimicrobial resistance in bacteria of animal origin. Basic Clin. Phar. Toxicology, vol. 96, no. 4, p. 271-281. https://doi.org/10.1111/j.1742-7843.2005.pto960401.x PMid:15755309
Bengtsson, B., Unnerstad, H. E., Ekman, T., Artursson, K., Nilsson-Ost, M., Waller, K. P. 2009. Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Vet. Microbiology, vol. 136, p. 142-149. https://doi.org/10.1016/j.vetmic.2008.10.024
Bradley, A.J., Leach, K.A., Breen, J.E. et al. 2007. Survey of the incidence and aetiology of mastitis on dairy farms in England and Wales. Vet Rec., vol. 160, no. 8, p. 253-257. https://doi.org/10.1136/vr.160.8.253
Foltys, V., Kirchrneová, K. 2005. Development of mastitis pathogens occurrence and their susceptibility to antibiotics in basic production of milk. Jour. of Farm Anim. Sci., vol. 38, p. 177-180.
Hameed, S., Arshad, M., Ashraf, M., Avais, M., Shahid, M. A. 2008. Prevalence of common mastitogens and their antibiotic susceptibility in tehsil Burewala, Pakistan. Pakistan Journal of Agricultural Science, vol. 45, no. 2, p.182-183.
Hortet, P., Beaudeau, F., Seegers, H. 1999. Reduction in milk yield associated with somatic cell counts up to 600 000 cells/ml in French Holstein cows without clinical mastitis. Livest. Prod. Sci., vol. 61, p. 33-42. https://doi.org/10.1016/S0301-6226(99)00051-2
Idriss, S. H., Foltys, V., Tančin, V., Kirchnerová, K., Tančinová, D. Zaujec, K. 2014. Mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Nitra. Slovakia. Slovak J. Anim. Sci. vol. 47, no. 1, p. 33-38.
Jackson, P., Cockcroft, P. 2002. Clinical Examination of Farm Animals. Oxford, UK, Blackwell Science Ltd, Wiley-Blackwell, p. 154-166. https://doi.org/10.1002/9780470752425
Kader, M. A., Samad, M. A., Saha, S., Taleb, M. A. 2002. Prevalence and etiology of mastitis with antibiotic sensitivity in milch cows of Bangladesh. Indian Jour. Dairy Sci., vol. 55, p. 218-223.
Malinowski, E., 2000. Mastitis prophylaxis and treatment during dry period - advantages and threats. Med. Weter., vol. 56, p. 759-763.
Kmeť, V., Bujňáková, D. 2018. Antimicrobial resistance escherichia coli isolated from calves, Journal of Microbiology Biotechnology and Food Science, vol. 7, no. 4, p. 412 - 415. https://doi.org/10.15414/jmbfs.2018.7.4.412-415
Lalrintluanga, C., Ralte, E. L., Markunga, H. 2003. Incidence of mastitis, bacteriology and antibiogram in dairy cows in Mizoram. Indian Vet. J., vol. 80, p. 931-932.
Malinowski, E., Gajewski, Z., 2009. Characteristics of cows mastitis caused by human foodborne pathogens. Życie Wet., vol. 84, p. 290-294.
Malinowski, E., Lassa, H., Kłossowska, A., Smulski, S., Markiewicz, H., Kaczmarowski, M., 2006. Etiological agents of dairy cows' mastitis in western part of Poland. Pol. J. Vet. Sci., vol. 9, p. 191-194. PMid:17020014
Medveďová, A., Valík, Ľ., Sirotná, Z.,Liptáková, D. 2009. Growth Characterization of Staphylococcus aureus in Milk: a Quantitative Approach. Czech Journal of Food Science, vol. 27, no. 6, p. 443-453.
Muhamed, H. M., Doss, A., Vijayasanthi, M., and Venkataswamy, R. 2012. Antimicrobial drug susceptibility of staphylococcus aureus from subclinical bovine mastitis in Coimbatore, Tamilnadu, South India. Vet. World., vol. 5 ,no. 6, p. 352-355. https://doi.org/10.5455/vetworld.2012.352-355
McDougall, S., 2003. Intramammary treatment of clinical mastitis of dairy cows with a combination of lincomycin and neomycin, or penicillin and dihydrostreptomycin. N. Z. Vet. J., vol. 51, no. 3, p.111-116. https://doi.org/10.1080/00480169.2003.36349
National committee for clinical laboratory standards (NCCLS). 2002. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Approved Standard. NCCLS Document M31-A2, Wayne, 2002, PA.
Østerås, O., Sølverød, L,. Reksen, O., 2006. Milk culture results in a large Norwegian survey-effects of season, parity, days in milk, resistance, and clustering. Jour. Dairy Sci., vol. 89, no. 3, p. 1010-1023. https://doi.org/10.3168/jds.S0022-0302(06)72167-1
Pittkala, A., Haveris, M., Pyorala, S., Myllys, V., Buzalski, T. H. 2004. Bovine mastitis in Finland 2001 - prevalence, distribution of bacteria and antimicrobial resistance. Journal of Dairy Science, vol. 87, no. 8, p. 2433-42. https://doi.org/10.3168/jds.S0022-0302(04)73366-4
Pyörälä, S., Taponen, S. 2009. Coagulase-negative staphylococci; emerging mastitis pathogens. Vet. Microbiol., vol. 134, p. 3-8. https://doi.org/10.1016/j.vetmic.2008.09.015
Shaheen, M., Tantary, H.A. and Nabi, S.U. 2016. A treatise on bovine mastitis: disease and disease economics, etiological basis, risk factors, impact on human health, therapeutic management, prevention and control strategy. J. Adv. Dairy Res., vol. 4, no. 1 p. 1-10. https://doi.org/10.4172/2329-888X.1000150
Seegers, H., Fourichon, Ch., Beaudeau, F. 2003. Production effects related to mastitis and mastitis economics in dairy cattle herds. Vet. Res., vol. 34, p. 475-491. https://doi.org/10.1051/vetres:2003027 PMid:14556691
Sampimon, O. H., Barkema, W., Berends, I., Sol, J., Lam, T. 2009. Prevalence of intramammary infection in Dutch dairy herds. Journal of Dairy Research, vol. 76, 2, p. 129-136. https://doi.org/10.1017/S0022029908003762
Sumathi, B. R., Veeragowda, B., Amitha, R. G. 2008. Prevalence and antibiogram profile of bacterial isolates from clinical bovine mastitis. Vet. World, vol. 1, no. 8, p. 237-238.
Tančin, V., Kirchnerová, K., Foltys, V., Mačuhova, L., Tančinová, D. 2006. Microbial contamination and somatic cell count of bovine milk striped and after udder preparation for milking. Slovak Journal of Animal Science, vol. 39, p. 214-217.
Vasiľ, M. 2009. Etiology, course and reduction of incidence of environment of dairy cows. Slovak Journal of Animal Science, vol. 42, 2009, p. 136-144.
Vršková, M., Tančin, V., Kirchnerová, K., Sláma, P., 2015. Evaluation of daily milk production in tsigai ewes by somatic cell count. Potravinarstvo, vol. 9, p. 206-210. https://doi.org/10.5219/439
Zadoks, R. N., Middleton, J. R., McDougall, S., Katholm, J., Schukken, Y. H. 2011. Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans. Journal of Mammary Gland Biology and Neoplasia, vol. 16, no. 4, p. 357-372. https://doi.org/10.1007/s10911-011-9236-y
Williams, R. 2000. The impact of antimicrobial resistance. Acta Veterinaria Scandinavica, Suppl., vol. 93, p. 17-20.
Zajác, P.,Tomáška, M., Murárová, A.,Čapla, J.,Čurlej, J. 2012. Quality and safety of raw cow's milk in slovakia in 2011. Potravinarstvo, vol. 6, no. 2, p. 64-73. https://doi.org/10.5219/189
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