Vicia villosa protein isolate: a new source of protein to make a biodegradable film


  • Farnaz Rezaiyan Attar Department of Food Science and Technology, Ferdowsi University of Mashhad (FUM), PO Box 91775-1163, Mashhad
  • Fatemeh Rezagholi Department of Food Engineering, Near East University, Nicosia, via Mersin 10, Cyprus
  • Mohammad Ali Hesarinejad Department of Food Processing, Research Institute of Food Science and Technology (RIFST), PO Box 91735-147, Mashhad, Iran



biodegradable film, glycerol, sorbitol, Vicia villosa protein isolate


Edible films from Vicia villosa protein isolate (VVPI) containing different contents of glycerol and sorbitol (30, 40, 50 and 60%w/w of protein) were developed. The aim of this study was to investigate the influence of type and concentration of plasticizers on the properties of edible films obtained from VVPI. Type and concentration of plasticizer significantly (p <0.05) affected the mechanical, barrier, thermal and surface properties as well as opacity of the films. As plasticizer concentration increased, tensile strength decreased concomitant with increase in elongation at break and water vapor permeability. The similar trend behavior was observed for the film solubility, which increased with increasing plasticizer concentration. Sorbitol plasticized films, showed higher film solubility compared to glycerol plasticized films. Sorbitol plasticized films provided the most tensile strength values; however, its effect on water vapor permeability was low. In contrast, glycerol plasticized films exhibited the least tensile strength values, resulting in increasing water vapor permeability. Opacity of glycerol plasticized films was lower than that of sorbitol plasticized films, and decreased with increasing plasticizer content (p <0.05). Also, a significant decrease (p <0.05) was observed in thermal features and surface hydrophobicity values with increasing in plasticizer contents. It was observed that the films plasticized with sorbitol had lower moisture content than those with glycerol.


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Aarssen, L. W., Hall, I. V., Jensen, K. I. N. 1986. The biology of Canadian weeds: 76. Vicia angustifolia L., V. cracca L., V. sativa L., V. tetrasperma (L.) Schreb. and V. villosa Roth. Canadian journal of plant science, vol. 66, no. 3, p. 711-737.

American Society for Testing and Materials. 1994. Standard test method for moisture content of paper and paperboard by oven drying. Designation: D644-94. In ASTM, Annual book of American Standard Testing Methods standards. p. 1-2. Pennsylvania : West Conshohochem.

American Society for Testing and Materials. 1995. Standard test methods for tensile properties of thin plastics sheeting D882-91. In ASTM, Annual Book of American Standard Testing Methods. p. 182-190. Pennsylvania : West Conshohochem.

Arvanitoyannis, I. S., Nakayama, A., Aiba, S. 1998. Chitosan and gelatin based edible films: state diagrams, mechanical and permeation properties. Carbohydrate Polymer, vol. 37, no. 4, p. 371-382.

Arvanitoyannis, I., Psomiadou, E., Nakayama, A., Aiba, S., Yamamoto, N. 1997. Edible films made from gelatin, soluble starch and polyols, Part 3. Food Chemistry, vol. 60, no. 4, p. 593-604.

Ashley, R. J. 1985. Permeability and plastics packaging. Polymer Permeability, p. 269-308.

Bamdad, F., Goli, A. H., Kadivar, M. 2006. Preparation and characterization of proteinous film from lentil (Lens culinaris): Edible film from lentil (Lens culinaris). Food Research International, vol. 39, no. 1, p. 106-111.

Barreto, P. L. M., Pires, A. T. N., Soldi, V. 2003. Thermal degradation of edible films based on milk proteins and gelatin in inert atmosphere. Polymer Degradation and Stability, vol. 79, no. 1, p. 147-152.

Bioplastics market data, 2015. European Bioplastics [online] s.a [cit 2018-02-01] Available at:

Bourtoom, T. 2008. Factors affecting the properties of edible film prepared from mung bean proteins. International Food Research Journal, vol. 15, no. 2, p. 167-180.

Cagri, A., Ustunol, Z., Ryser, E. T. 2004. Antimicrobial edible films and coatings. Journal of food protection, vol. 67, no. 4, p. 833-848. PMid:15083740

Cuq, B., Gontard, N., Cuq, J. L., Guilbert, S. 1997. Selected functional properties of fish myofibrillar protein-based films as affected by hydrophilic plasticizers. Journal of Agricultural and Food Chemistry, vol. 45, no. 3, p. 622-626.

De Carvalho, R. A., Grosso, C. R. F. 2004. Characterization of gelatin based films modified with transglutaminase, glyoxal and formaldehyde. Food hydrocolloids, vol. 18, no. 5, p. 717-726.

Donhowe, I. G., Fennema, O. 1993. The effects of plasticizers on crystallinity, permeability, and mechanical properties of methylcellulose films. Journal of Food Processing and Preservation, vol. 17, no. 4, p. 247-257.

Gennadios, A., Weller, C. L., Testin, R. F. 1993a. Property modification of edible wheat, gluten-based films. ransactions of the ASAE, vol. 36, no. 2, p. 465-470.

Gennadios, A., Weller, C., Testin, R. F. 1993b. Temperature effect on oxygen permeability of edible protein‐based films. Journal of Food Science, vol. 58, no. 1, p. 212-214.

Ghanbarzadeh, B., Oromiehi, A. R. 2008. Studies on glass transition temperature of mono and bilayer protein films plasticized by glycerol and olive oil. Journal of Applied Polymer Science, vol. 109, no. 5, p. 2848-2854.

Ghasemlou, M., Khodaiyan, F., Oromiehie, A. 2011. Physical, mechanical, barrier, and thermal properties of polyol-plasticized biodegradable edible film made from kefiran. Carbohydrate Polymers, vol. 84, no. 1, p. 477-483.

Gleason, H. A., Cronquist, A. 1963. Manual of vascular plants of northeastern United States and adjacent Canada. New York, USA : New York Botanical Garden Pr Dept. 910 p. ISBN-13: 978-0893273651.

Gontard, N., Duchez, C., Cuq, J., Guilbert, S. 1994. Edible composite films of wheat gluten and lipids: water vapour permeability and other physical properties. International Journal of Food Science & Technology, vol. 29, no. 1, p. 39-50.

Gontard, N., Guilbert, S., Cuq, J. 1992. Edible wheat gluten films: influence of the main process variables on film properties using response surface methodology. Journal of food science, vol. 57, no. 1, p. 190-195.

Gontard, N., Guilbert, S., Cuq, J. 1993. Water and glycerol as plasticizers affect mechanical and water vapor barrier properties of an edible wheat gluten film. Journal of Food Science, vol. 58, no. 1, p. 206-211.

Guilbert, S. 1986. Food packaging and preservation. Theory and Practice in Technology and Application of Edible Protective Films. London, UK : Elsevier Applied Science Publishing Co. p. 371.

Hagenmaier, R. D., Shaw, P. E. 1990. Moisture permeability of edible films made with fatty acid and hydroxypropyl methyl cellulose. Journal of Agricultural and Food Chemistry, vol. 38, no. 9, p. 1799-1803.

Haugaard, V. K., Udsen, A., Mortensen, G., Høegh, L., Petersen, K., Monahan, F. 2001. Potential Food Applications of Biobased Materials. An EU‐Concerted Action Project. Starch‐Stärke, vol. 53, no. 5, p. 189-200.<189::AID-STAR189>3.0.CO;2-3

Horowitz, W., Latimer, G. 1994. Official methods of AOAC International. 18th ed. Maryland, USA.

Hulten, E. 1968. Flora of Alaska and neighboring territories: a manual of the vascular plants. Stanford, California : Stanford University Press. 1032 p. ISBN 9780804706438.

Chae, S. I., Heo, T. R. 1997. Production and properties of edible film using whey protein. Biotechnology and Bioprocess Engineering, vol. 2, no. 2, p. 122-125.

Chang, C., Nickerson, M. T. 2014. Effect of plasticizer-type and genipin on the mechanical, optical, and water vapor barrier properties of canola protein isolate-based edible films. European Food Research and Technology, vol. 238, no. 1, p. 35-46.

Chick, J., Ustunol, Z. 1998. Mechanical and barrier properties of lactic acid and rennet precipitated casein‐based edible films. Journal of Food Science, vol. 63, no. 6, p. 1024-1027.

Cho, S. Y., Rhee, C. 2002. Sorption characteristics of soy protein films and their relation to mechanical properties. LWT - Food Science and Technology, vol. 35, no. 2, p. 151-157.

Choi, W., Han, J. H. 2001. Physical and mechanical properties of pea‐protein‐based edible films. Journal of Food Science, vol. 66, no. 2, p. 319-322.

Jongjareonrak, A., Benjakul, S., Visessanguan, W., Tanaka, M. 2006. Effects of plasticizers on the properties of edible films from skin gelatin of bigeye snapper and brownstripe red snapper. European Food Research and Technology, vol. 222, no. 3-4, p. 229-235.

Kester, J. J., Fennema, O. R. 1986. Edible films and coatings: a review. Food technology, vol. 40, p. 47-59.

Kwok, D. Y., Neumann, A. W. 1999. Contact angle measurement and contact angle interpretation. Advances in colloid and interface science, vol. 81, no. 3, p. 167-249.

Lieberman, E. R., Gilbert, S. G. 1973. Gas permeation of collagen films as affected by cross‐linkage, moisture, and plasticizer content, in: Journal of Polymer Science: Polymer Symposia, vol. 41, no. 1, p. 33-43.

Liu, C. C., Tellez-Garay, A. M., Castell-Perez, M. E. 2004. Physical and mechanical properties of peanut protein films. LWT-Food Science and Technology, vol. 37, no.7, p. 731-738.

Ma, X., Chang, P. R., Yu, J. 2008. Properties of biodegradable thermoplastic pea starch/carboxymethyl cellulose and pea starch/microcrystalline cellulose composites. Carbohydrate Polymers, vol. 72, no. 3, p. 369-375.

Mannheim, C. H., Passy, N. 1990. Interaction between packaging materials and foods. Packaging Technology and Science, vol. 3, no. 3, p. 127-132.

McHugh, T. H., Aujard, J., Krochta, J. M. 1994. Plasticized whey protein edible films: water vapor permeability properties. Journal of Food Science, vol. 59, no. 2, p. 416-419.

McHugh, T. H., Avena‐Bustillos, R., Krochta, J. M. 1993. Hydrophilic edible films: modified procedure for water vapor permeability and explanation of thickness effects. Journal of food science, vol. 58, no. 4, p. 899-903.

McHugh, T. H., Krochta, J. M. 1994. Sorbitol-vs glycerol-plasticized whey protein edible films: integrated oxygen permeability and tensile property evaluation. Journal of Agricultural and Food Chemistry, vol. 42, no. 4, p. 841-845.

Myers, A. W., Meyer, J. A., Rogers, C. E., Stannett, V., Szwarc, M. 1961. Studies in the gas and vapor permeability of plastic films and coated papers. The permeation of water vapor. Tappi Journal, vol. 45, p. 63-77.

Orliac, O., Rouilly, A., Silvestre, F., Rigal, L. 2003. Effects of various plasticizers on the mechanical properties, water resistance and aging of thermo-moulded films made from sunflower proteins. Industrial Crops and Products, vol. 18, no. 2, p. 91-100.

Osés, J., Fernández-Pan, I., Mendoza, M., Maté, J. I. 2009. Stability of the mechanical properties of edible films based on whey protein isolate during storage at different relative humidity. Food Hydrocolloids, vol. 23, no. 1, p. 125-131.

Otoni, C. G., Avena-Bustillos, R. J., Olsen, C. W., Bilbao-Sáinz, C., McHugh, T. H. 2016. Mechanical and water barrier properties of isolated soy protein composite edible films as affected by carvacrol and cinnamaldehyde micro and nanoemulsions. Food Hydrocolloids, vol. 57, p. 72-79.

Park, H. J. 1999. Development of advanced edible coatings for fruits. Trends in Food Science & Technology, vol. 10, no. 8, p. 254-260.

Park, H. J., Chinnan, M. S. 1990. Properties of edible coatings for fruits and vegetables. Paper-American Society of Agricultural Engineers, vol. 19, p. 90-6510.

Petersen, K., Nielsen, P. V., Bertelsen, G., Lawther, M., Olsen, M. B., Nilsson, N. H., Mortensen, G. 1999. Potential of biobased materials for food packaging. Trends in Food Science & Technology, vol. 10, no. 2, p. 52-68.

Ramos, Ó. L., Reinas, I., Silva, S. I., Fernandes, J. C., Cerqueira, M. A., Pereira, R. N., Vicente, A. A., Pocas, M. F., Pintado, M. E., Malcata, F. X. 2013. Effect of whey protein purity and glycerol content upon physical properties of edible films manufactured therefrom. Food Hydrocolloids, vol. 30, no. 1, p. 110-122.

Rhim, J. W., Ng, P. K. W. 2007. Natural biopolymer-based nanocomposite films for packaging applications. Critical reviews in food science and nutrition. vol. 47, no. 4, p. 411-433. PMid:17457725

Rodríguez, M., Oses, J., Ziani, K., Mate, J. I. 2006. Combined effect of plasticizers and surfactants on the physical properties of starch based edible films. Food Research International, vol. 39, n. 8, p. 840-846.

Roy, L., Mulligan, Taylor, G. A. 1968. Flora of the Queen Charlotte Islands. Part 2. Cytological aspects of the vascular plants. Ottawa : Canada Department of Agriculture.

Ryan, M., McEvoy, E., Duignan, S., Crowley, C., Fenelon, M., O'Callaghan, D. M., FitzGerald, R. J. 2008. Thermal stability of soy protein isolate and hydrolysate ingredients. Food chemistry, vol. 108, no. 2, p. 503-510. PMid:26059128

Ryu, S. Y., Rhim, J. W., Roh, H. J., Kim, S. S. 2002. Preparation and physical properties of zein-coated high-amylose corn starch film. LWT - Food Science and Technology, vol. 35, no. 8, p. 680-686.

Saremnezhad, S., Azizi, M. H., Barzegar, M., Abbasi, S., Ahmadi, E. 2010. Properties of a new edible film made of faba bean protein isolate. Journal of Agricultural Science and Technology, vol. 13, no. 2, p. 181-192.

Shafiei, A., Kazemi, E., Mozafari, S., Monapooyeh, A., Shah Moradi, A. 2006. Autecology of Vicia villosa in Kohgiloyeh-va-Boyerahmad province. Agricultural Scientific Information and Documentation Centre, Agricultural Research and Education Organization. [online] s.a [cit 2018-02-01] Available at:

Shahraki, F., Hadad-Khodaparast, M. H., Hesarinejad, M. A., Mortazavi, S. A., Milani, E., Hoseinzadeh, A. 2013. The quality and purity of the proteins extracted from the Lathyrus sativus seeds and optimization by the response surface methodology (RSM). In 1st International e-Conference on Novel Food Processing. Mashhad, Iran : Ferdowsi University of Mashhad. p. 4.

Sobral, P. J. A., Menegalli, F. C., Hubinger, M. D., Roques, M. A. 2001. Mechanical, water vapor barrier and thermal properties of gelatin based edible films. Food Hydrocolloids, vol. 15, no. 4-6, p. 423-432.

Sobral, P. J. A., Monterrey-Q, E. S., Habitante, A. M. Q. B. 2002. Glass transition study of Nile Tilapia myofibrillar protein films plasticized by glycerin and water. Journal of Thermal Analysis and Calorimetry, vol. 67, no. 2, p. 499.

Soliman, E. A., Tawfik, M. S., El-Sayed, H., Moharram, Y. G. 2007. Preparation and characterization of soy protein based edible/biodegradable films. American Journal of Food Technology, vol. 2, no. 6, p. 462-476.

Stuchell, Y. M., Krochta, J. M. 1994. Enzymatic treatments and thermal effects on edible soy protein films. Journal of Food Science, vol. 59, no. 6, p. 1332-1337.

Tanaka, M., Iwata, K., Sanguandeekul, R., Handa, A., Ishizaki, S. 2001. Influence of plasticizers on the properties of edible films prepared from fish water‐soluble proteins. Fisheries science, vol. 67, no. 2, p. 346-351.

Tang, C. H., Jiang, Y. 2007. Modulation of mechanical and surface hydrophobic properties of food protein films by transglutaminase treatment. Food research international, vol. 40, no. 4, p. 504-509.

Tavili, A., Shafiei, A., Pouzesh, H., Farajollahi, A., Saberi, M., Shahmoradi, A. A. 2010. Autecology of Vicia villosa in Kohgiluyeh and Boyerahmad Province, Iran. Rangeland Journal, vol. 4, no. 3, p. 422-433.

Vanin, F. M., Sobral, P. J. A., Menegalli, F. C., Carvalho, R. A., Habitante, A. 2005. Effects of plasticizers and their concentrations on thermal and functional properties of gelatin-based films. Food Hydrocolloids, vol. 19, no. 5, p. 899-907.

Vogler, E. A. 1998. Structure and reactivity of water at biomaterial surfaces. Advances in colloid and interface science, vol. 74, no. 1-3, p. 69-117.

Wittaya, T. 2013. Influence of type and concentration of plasticizers on the properties of edible film from mung bean proteins. Current Applied Science and Technology Journal, vol. 13, no. 1, p. 51-58.

Yang, L., Paulson, A. T. 2000. Mechanical and water vapour barrier properties of edible gellan films. Food Research International, vol. 33, no. 7, p. 563-570.




How to Cite

Rezaiyan Attar, F. ., Rezagholi, F. ., & Hesarinejad, M. A. . (2018). Vicia villosa protein isolate: a new source of protein to make a biodegradable film. Potravinarstvo Slovak Journal of Food Sciences, 12(1), 461–471.

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