Characterization of Tunisian castor bean genotypes using SDS-PAGE of total seed storage proteins
Keywords:castor, Tunisian genotypes, dendrogram, protein markers, SDS-PAGE
Due to the chemical and physical properties of castor oil (Ricinus communis L.) that make it a valuable raw material for numerous industrial applications, including the production of biofuel, interest to develop more and better varieties has been increased. The objectives of this work, were to find out the level of genetic variability present in 56 Tunisian castor germplasm by using the electrophoretic profiles of total seed proteins with different molecular weights through SDS-PAGE. Fifty-six castor (Ricinus communis L.) genotypes were used in the present study. Seeds of castor were obtained from the University of Carthage, National Institute of Research in Rural Engineering, Waters and Forests (INRGREF), Regional Station of Gabès, Tunisia. Storage proteins were extracted from individual grains by the standard reference electrophoretic method by ISTA in the presence of sodium dodecyl sulfate (SDS-PAGE). The number of total scorable protein bands was twentynine as a result of SDS-PAGE technique but those that were not cosistent in reproducibility and showed occasional variation in sharpness and density were not considered. Out of twentynine polypeptide bands, 5 (18%) were commonly present in all accessions and considered as monomorphic, while 24 (82%) showed variations and considered as polymorphic. The size of the protein bands obtained through SDS-PAGE ranged from 30 to 180 kDa. On the basis of banding profiles of proteins of different kDa, gel was divided into zones A, B and C. The major protein bands were lied in zones A and B, while minor bands were present in zones C. The dendrogram tree demonstrated the relationship among the 56 Tunisian castor genotypes according to the similarity index, using UPGMA cluster analysis. The dendrogram was divided into three main clusters. Similarly the present study of genetic variability in the seed storage polypeptide determined by SDS-PAGE technique proved that it is fruitful to identify genetic diversity among accessions of castor.
AL-Huqail, A. A., Abdelhaliem, E. 2015. Evaluation of Genetic Variations in maize Seedlings Exposed to Electric Field Based on Protein and DNA Markers. BioMed Research International, vol. 2015, p. 1-15. https://doi.org/10.1155/2015/874906
Arslan, M. 2012. Evaluation of genetic similarity and agronomic traits of Castor bean populations naturally grown in the Eastern Mediterranean region of Turkey. Biotechnology & Biotechnological Equipment, vol. 26, no. 4, p. 3089-3093. https://doi.org/10.5504/BBEQ.2012.0034
Aslani, M. R., Maleki, M., Mohri, M., Sharifi, K., Najjar-Nezhad, V., Afshari, E. 2007. Castor bean (Ricinus communis) toxicosis in a sheep flock. Toxicon, vol. 49, no. 3, p. 400-406. https://doi.org/10.1016/j.toxicon.2006.10.010
Brandon, D. L., McKeon, T. A., Patfield, S. A., Kong, Q., He, X. 2016. Analysis of Castor by ELISAs that distinguish Ricin and Ricinus communis agglutinin (RCA). J Am Oil Chem Soc., vol. 93, p. 359-363. https://doi.org/10.1007/s11746-015-2773-6
Doan, L. G. 2004. Ricin: mechanism of toxicity, clinical manifestations, and vaccine development. Review J. Toxicol., vol. 42, p. 201-208. https://doi.org/10.1081/CLT-120030945
Gregova, E., Šliková, S., Hozlár, P. 2015. Seed protein electrophoresis for identification of oat registered cultivars. Potravinarstvo, vol. 9, no. 1, p. 411-416. https://doi.org/10.5219/513
Hartley, M. R. and Lord, J. M. 2004. Cytotoxic ribosome-inactivating lectins from plants. Biochim. Biophys. Acta, vol. 1701, p. 1-14. https://doi.org/10.1016/j.bbapap.2004.06.004
Cheema, N. M., Malik, M. A., Qadir, G., Rabbani, M. A. 2010. Characterization of castor bean genotypes under various environments using SDS-PAGE of total seed storage proteins. Pak. J. Bot., vol. 42, no. 3, p. 1797-1805.
Iqbal, J., Shinwari, Z. K., Rabbani, M. A., Khan, S. A. 2014. Genetic variability assessment of maize (Zea mays L.) germplasm based on total seed storage proteins banding pattern using SDS-PAGE. European academic research, vol. 2, no. 2, p. 2144-2160.
Iqbal, J., Shinwari, Z. K., Rabbani, M. A. 2014. Investigation of total seed storage proteins of Pakistani and Japanese maize (Zea mays L.) through SDS-PAGE markers. Pakistan Journal of Botany, vol. 46, no. 3, p. 817-822.
Johnson, R. C., Lemire, S. W., Woolfitt, A. R., Ospina, M., Preston, K. P., Olson, C. T., Barr, J. R. 2005. Quantification of ricinin in rat and human urine: a biomarker for ricin exposure. J. Anal. Toxicol., vol. 29, p. 149-155. https://doi.org/10.1093/jat/29.3.149
Kačmárová, K., Lavová, B., Socha, P., Urminská, D. 2016. Characterization of protein fractions and antioxidant activity of Chia seeds (Salvia Hispanica L.). Potravinarstvo, vol. 10, no. 1, p. 78-82. https://doi.org/10.5219/563
Khan, A. H., Khan, N., Minhas, N. M., Ghafoor, A., Rabbani, M. A. 2014. Diversity in seed storage proteins in maize genetic resources: I. variation in alcohol soluble zein protein fraction. International Journal of Agricurtural and Biological Engineering, vol. 16, p. 1015-1018.
Kumar, O., Nashikkar, A. B., Jayaraj, R., Vijayaraghavan, R., Prakash, A. O. 2004. Purification and biochemical characterisation of Ricin from castor seeds. Defense Sci. J., vol. 54, no. 3, p. 345-351. https://doi.org/10.14429/dsj.54.2048
Lacerda, R. S., Makishi, G. L. A., Chambib, H. N. M., Bittante, A. M. Q. B., Gomide, C. A., Costa, P. A., Sobral, P. J. A. 2014. Castor Bean (Ricinus communis) Cake Protein Extraction by Alkaline Solubilization: Definition of Process Parameters. Chemical engineering transactions, vol. 37, p. 775-780.
Lord, J. M., Roberts, L. M. and Robertus J. D. 1994. Ricin: structure, mode of action and some current applications. FASEB J., vol. 8, p. 201-208. https://doi.org/10.1096/fasebj.8.2.8119491
Madail, J. C. M., Belarmino, L. C., Neutzling, D. M. 2007. Aspectos Econômicos da mamona. Sistemas de Produção. Embrapa, 11. ISSN 1806-9207.
Malook, I., Jamil, M., Fayyaz, M., Rehman, H., Irshad, M., Ul Bashar, K., Khan, S., Akhter, N., Aslam, M. M., Rha, E. S. 2016. Biochemical and molecular characterization of Castor Bean (Ricinus Communis L.) collected from different climatic zones of Pakistan. Journal of Bio-Molecular Sciences, vol. 4, no. 1, p. 16-23.
Osman, G., Munshi, A., Altf, F., Mutawie, H. 2013. Genetic variation and relationships of Zea mays and Sorghum species using RAPD-PCR and SDS-PAGE of seed proteins. African Journal of Biotechnology, vol. 12, no. 27, p. 4269-4276. https://doi.org/10.5897/AJB12.2644
Rao, P. S., Keshavulu, K., Reddy B. K., Reddy M. N., Ankaiah, R. 2017. Characterization of Castor Bean Genotypes Using SDS-Page of Total Soluble Seed Proteins, Int. J. Pure App. Biosci.,vol. 5, no. 6, p. 132-137. https://doi.org/10.18782/2320-7051.2994
Riaz, S., Farrukh, M. A. 2014. Toxicological analysis of Ricin in medicinal castor oil with evaluation of health hazards. Asian Journal of Chemistry, vol. 26, no. 2, p. 499-503. https://doi.org/10.14233/ajchem.2014.15596
Schieltz, D. M., McWilliams, L. G., Kuklenyik, Z., Prezioso, S. M., Carter, A. J., Williamson, Y. M., McGrath, S. C., Morse, S. A., John R. Barr, J. R. 2015. Quantification of ricin, RCA and comparison of enzymatic activity in 18 Ricinus communis cultivars by isotope dilution mass spectrometry, Toxicon, vol. 95, p. 72-83. https://doi.org/10.1016/j.toxicon.2015.01.003
Socha, P., Tomka, M., Kačmárová, K., Lavová, B., Ivanišová, E., Mickowska, B., Urminská, D. 2016. Comparable efficiency of different extraction protocols for wheat and rye prolamins. Potravinarstvo, vol. 10, no. 1, p. 139-144. https://doi.org/10.5219/540
Vivodík, M., Balážová, Ž., Gálová, Z. and Petrovičová, L. 2018. Study of polymorphism of maize using DNA and protein markers. Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 157-165. https://doi.org/10.5219/874
Yoon, J. W., Jung, J. Y., Chung, H. J., Kim, M. R., Kim, Ch. W., Lim, S. T. 2010. Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins. Journal of Cereal Science, vol. 52, no. 2, p. 321-326. https://doi.org/10.1016/j.jcs.2010.06.015
Wrigley, C. W. 1992. Identification of cereal varieties by gel electrophoresis of the grain proteins. Heidelberg: Springer - Verlag, p. 17-41. https://doi.org/10.1007/978-3-662-01639-8_2
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