Comparison of two methods of protein quality evaluation in rice, rye and barley as food protein sources in human nutrition
Keywords:PDCAAS, DIAAS, protein quality, amino acid, ileal
Different foods differ in their protein quality, which is characterized by the content and digestibility of individual amino acids. The Food and Agriculture Organisation has recommended replacing the method for protein quality evaluation of foods called protein digestibility corrected amino acid score (PDCAAS) with the new method - digestible indispensable amino acid score (DIAAS), in which the values of ileal amino acid digestibility obtained in pigs are used. However, the information about DIAAS values of foods are limited. Therefore, the study on growing pigs was conducted to determine true fecal protein digestibility and standardized ileal amino acid digestibility of rice, rye and barley. Using these values, the PDCAAS and DIAAS were calculated and compared. A total of 18 gilts with a T-cannula inserted in the terminal ileum were allotted to 3 diets with six replicate pigs per diet. Three semi-purified diets were formulated to contain the tested cereal grains (rice, rye, barley) as the sole nitrogen source. Chromic oxide was used as indigestible marker. Each experimental period comprised of a 7-d adaptation period followed by 24 h collection of feces and ileal digesta. The content of nitrogen, dry matter and chromic oxide was analyzed in samples of diets, feces and ileal digesta. Moreover, in the samples of diets and ileal digesta the content of amino acids was determined. Calculated ratio of crude protein to lysine was greatest in rice (4.50) followed by rye (3.65) and the lowest one in barley (3.35). True fecal protein digestibility was greater when compared with ileal amino acid digestibility for all tested samples, thus suggesting an overestimation of protein quality determined by PDCAAS. Calculated PDCAAS values for rice, rye and barley (81, 65 and 61%) were generally greater than the DIAAS values (79, 56 and 55%), especially for the poorer quality protein sources such as rye and barley in comparison with rice. The lysine was the first limiting amino acid in all tested cereal grains. Based on the DIAAS evaluation, rice is better protein source in human nutrition in comparison with rye or barley.
Abelilla, J. J., Liu, Y., Stein, H. H. 2018. Digestible indispensable amino acid score (DIAAS) and protein digestibility corrected amino acid score (PDCAAS) in oat protein concentrate measured in 20 to 30 kilogram pigs. Journal of Science Food and Agriculture, vol. 98, no. 1, p. 410-414. https://doi.org/10.1002/jsfa.8457
Association of Official Analytical Chemists (AOAC). 1990. Official Methods of Analysis. 15th ed. AOAC, Arlington, VA.
Brestenský, M., Nitrayová, S., Patráš, P., Nitray, J. 2018. Dietary requirements for protein and amino acids in human nutrition. Current Nutrition and Food Science, vol. 14, p. 1-8. https://doi.org/10.2174/1573401314666180507123506
Cervantes-Pahm, S. K., Liu, Y., Stein, H. H. 2014. Digestible indispensable amino acid score and digestible amino acids in eight cereal grains. British Journal of Nutrition, vol. 111, no. 9, p. 1663-1672. https://doi.org/10.1017/S0007114513004273
Columbus, D., de Lange, C. F. M. 2012. Evidence for validity of ileal digestibility coefficients in monogastrics. British Journal of Nutrition, vol. 108 (Suppl S2), p. 264-72. https://doi.org/10.1017/S0007114512002334
Darragh, A. J., Hodgkinson, S. M. 2000. Quantifying the digestibility of dietary protein. Journal of Nutrition, vol. 130, no. 7, p. 1850-1856. https://doi.org/10.1093/jn/130.7.1850S
Deglaire, A., Bos, C., Tomé, D., Moughan, P. 2009. Ileal digestibility of dietary protein in the growing pig and adult human. British Journal of Nutrition, vol 102, no. 12, p. 1752-1759. https://doi.org/10.1017/S0007114509991267
FAO. 1991. Protein quality evaluation. Report of Joint FAO/WHO Expert Consultation. FAO Food and nutrition Paper 51, Food and Agriculture Organization, Rome.
FAO. 2013. Dietary protein quality evaluation in human nutrition. Report of an FAO Expert Consultation. FAO Food and Nutrition Paper 92, Food and Agriculture Organization, Rome.
Fuller, M. F., Tomé, D. 2005. In vivo determination of amino acid bioavailability in humans and model animals. Journal of AOAC International, vol. 88, no. 3, p. 923-934.
Gilani, G. S. 2012. Background on international activities on protein quality assessment of foods. British Journal of Nutrition, vol. 108 (Suppl S2), p. 168-182. https://doi.org/10.1017/S0007114512002383
Graham, H., Fadel, J. G., Newman, C. W., Newman, R. K. 1989. Effect of pelleting and beta-glucanase supplementation on the ileal and fecal digestibility of a barley-based diet in the pig. Journal of Animal Science, vol. 67, no. 5, p. 1293-1298. https://doi.org/10.2527/jas1989.6751293x
Hendriks, W. H., van Baal, J., Bosch, G. 2012. Ileal and faecal protein digestibility measurement in humans and other non-ruminants - a comparative species view. British Journal of Nutrition, vol. 108, (Suppl S2), p. 247-257. https://doi.org/10.1017/S0007114512002395
Jansman, A. J. M., Smink, W., Leeuwen, P., Rademacher, M. 2002. Evaluation through literature data of the amount and amino acid composition of basal endogenous crude protein at the terminal ileum of pigs. Animal Feed Science and Technology, vol. 98, no. 1-2, p. 49-60. https://doi.org/10.1016/S0377-8401(02)00015-9
Jondreville, C., van den Broecke, J., Gatel, F., Grosjean, F., van Cauwenberghe, S., Sève, B. 2001. Ileal digestibility of amino acids and estimates of endogenous amino acid losses in pigs fed wheat, triticale, rye, barley, maize and sorghum. Animal Research, vol. 50, no. 2, p. 119-134. https://doi.org/10.1051/animres:2001120
Mathai, J. K., Liu, Y., Stein, H. H. 2017. Values for digestible indispensable amino acid scores (DIAAS) for some dairy and plant proteins may better describe protein quality than values calculated using the concept for protein digestibility-corrected amino acid scores (PDCAAS). Britisch Journal of Nutrition, vol. 117, no. 4, p. 490-499. https://doi.org/10.1017/S0007114517000125
Moughan, P. J. 2003. Amino acid availability - aspects of chemical analysis and bioassay methodology. Nutrition Research Reviews, vol. 16, no. 2, p. 127-141. https://doi.org/10.1079/NRR200365
Moughan, P. J., Donkoh, A. 1991. Amino acid digestibility in non-ruminants: a review. Recent Advances in Animal Nutrition - Australia, p. 172-184.
Rowan, A. M, Moughan, P. J., Wilson, M. N., Maher, K., Tasman-Jones, C. 1994. Comparison of the ileal and faecal digestibility of dietary amino acids in adult humans and evaluation of the pig as a model animal for digestion studies in man. British Journal of Nutrition, vol. 71, no. 1. p. 29-42. https://doi.org/10.1079/BJN19940108
Schaafsma, G. 2012. Advantages and limitations of the protein digestibility-corrected amino acid score (PDCAAS) as a method for evaluating protein quality in human diets. British Journal of Nutrition, vol. 108, no. 2, 333-336. https://doi.org/10.1017/S0007114512002541
Stein, H. H., Seve, B., Fuller, M. F., Moughan, P. J., de Lange, C. F. M. 2007. Invited review: Amino acid bioavailability and digestibility in pig feed ingredients: terminology and application. Journal of Animal Science, vol 85, no. 1, p. 172-180. https://doi.org/10.2527/jas.2005-742
Whiting, F., Bezeau, L. M. 1957. The metabolic fecal nitrogen excretion of the pig as influenced by the amount of fibre in the ration and body weight. Canadian Journal of Animal Science, vol. 37, no. 1, p. 95-105. https://doi.org/10.4141/cjas57-014
Williams, C. H., David, D. J., Lismaa, O. 1962. The determination of chromic oxide in fecal samples by atomic absorption spectrophotometry. Journal Agricultural Science, vol. 59, no. 3, p. 381-385. https://doi.org/10.1017/S002185960001546X
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